Screening of new 2,4- and 2,6-dinitroaniline derivates for phytotoxicity and antimitotic activity

Ozheredov, S. P.; Yemets, А. І.; Brytsun, V. M.; Ozheredova, I. P.; Lozynsky, M. O.; Blume, Ya. B.

doi:10.3103/s0095452709050016

Cited by 11 publications

(6 citation statements)

References 22 publications

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“…Podwyszyńska et al (2017) showed that the efficiency of apple somatic polyploidization by different reagents was highly dependent on cultivar. Some researchers reported using 2.4-D as a chromosome doubling agent (Ozheredov et al 2009;Singh 1976). However, in this study, we applied 2,4-D for chromosome doubling in Hedychium species, but no positive result was obtained.…”

Section: Discussionmentioning

confidence: 58%

Testing two chromosome doubling agents for in vitro tetraploid induction on ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig

Mohammadmehdi

Shukurova

Watanabe

2021

In Vitro Cell.Dev.Biol.-Plant

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Polyploidization can be a way to produce new varieties in vegetatively propagated species where options on increasing genetic variability are limited compared with sexual reproduction. While there are hundreds of publications with in vitro methods in somatic doubling, it is cardinal to custom-test for a target species of interest on choosing specific reagents and optimizing conditions. This research was performed to provide a reference process for Zingiberaceae species of which the majority is reproduced with vegetative propagation. Ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig, were employed to optimize chromosome doubling for tetraploid production as they are typically used by vegetative propagation. However, they have the popularity as ornamentals globally due to their horticultural aspects. Tetraploid induction was optimized by in vitro somatic chromosome doubling on H. coronarium and H. gardnerianum through callogenesis. The explant segments of young leaf blades or leaf sheaths were treated with different concentrations of either with colchicine or with oryzalin. The regenerated shoots from callus cultures were transferred to basal MS medium for 2mo to confirm somatic stability. The ploidy was assessed by flow cytometry, measuring the size and density of stomata, counting chromosomes, and chloroplasts in guard cells. The highest percentage of tetraploid regenerated plants was observed with 1250 µM colchicine treatment for Hedychium gardnerianum when compared to the other treatments tested, while no tetraploid plants were obtained from the oryzalin treatment. In H. coronarium, four mixoploid regenerated plants treated with 15 µM oryzalin were confirmed and no shoots were obtained from the colchicine treatment. The results indicated that the in vitro polyploid induction is optimized with the two Hedychium species, which could reference other Zingiberaceae species.

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Section: Discussionmentioning

confidence: 58%

Testing two chromosome doubling agents for in vitro tetraploid induction on ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig

Mohammadmehdi

Shukurova

Watanabe

2021

In Vitro Cell.Dev.Biol.-Plant

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“…Previously, in order to determine the phytotoxicity level and affinity to plant tubulins of more than 100 newly synthesised derivatives of dinitroaniline, a series of experiments were conducted by our group (Ozheredov et al, 2009;Ozheredov et al, 2010). Testing with Allium showed that phytotoxic effect of typical dinitroaniline compounds (trifluralin) appeared even at concentration 1 µM.…”

Section: Discussionmentioning

confidence: 99%

Induction of Polyploidy in Giant Miscanthus (Miscanthus × Giganteus Greef Et Deu.)

Melnychuk

Ozheredov

Rakhmetov

et al. 2020

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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Results of obtaining miscanthus polyploid lines using both classic and new dinitroanilines to improve productivity of biomass as well as quality for biofuel production are represented. All of compounds applied in the study appeared to be able to induce poyploidy in Miscanthus × giganteus.It was found that new antimitotics have a lower phytotoxicity level compared to classic dinitroani-lines. Ploidy level of obtained lines was confirmed by cytological studies using light microscopy. These lines are acclimatised for growing in open soil conditions. Currently, morphometric parameters of selected lines are being studied and analysed.

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“…Thus, A. cepa was used to study the toxic properties of synthetic organic compounds, e.g. derivatives of 2,4-and 2,6-dinitroanilines [39], two new complexes of silver(I) with sulfachloropyridazine [36], N-nitrosodiethylamine [15], drugs [1], pesticides [18; 38]. The aim of this work was to generalize the methodological approaches of using A. cepa in the phytotesting of toxicants.…”

Section: Formulation Of the Problemmentioning

confidence: 99%

Onion (Allium Cepa L.) as a Test Plant

Tkachuk¹,

Zelena²

2023

BHT

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Phytotesting has long been used to determine the quality of seeds, agricultural soil fertility, in biomedical research, and recently in the field of environmental protection to assess the ecological quality of natural environment (water, soil) in phytotesting of toxicants. Various plant representatives are used as model test organisms, in particular, onion (Allium cepa L.) is considered as a standard test plant for the determination of toxicants. The purpose of the work is to generalize methodological approaches of using onion (Allium cepa L.) in phytotesting of toxicants. Methodology. During the research, the following methods were used: 1) general scientific methods (methods of theoretical research of available information); 2) analytical and generalized methods (for the analysis of scientific and literature sources on the given problem); 3) empirical (for accumulating facts); 4) methods of argumentation (to prove one’s own judgments). Scientific novelty - methodical approaches of using onion in phytotesting of toxicants are summarized, formulas for calculating phytotoxic indices (root length index, phytotoxic effect of solutions, toxicity index of solutions for each test function, average toxicity index of the tested solutions) are presented. Conclusions - phytotesting is a commonly used method of assessing the quality of natural environment (water, soil) which is based on the sensitivity of plants to external chemical influences and is reflected in growth and morphological characteristics. The standard test plant for the determination of toxicant influence is the onion (Allium cepa), which can be used both in the growth test and in the Allium-test. The most sensitive characteristics of onion are the mitotic activity of the apical meristem cells and the frequency of cells with chromosomal aberrations.

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Screening of new 2,4- and 2,6-dinitroaniline derivates for phytotoxicity and antimitotic activity

Cited by 11 publications

References 22 publications

Testing two chromosome doubling agents for in vitro tetraploid induction on ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig

Testing two chromosome doubling agents for in vitro tetraploid induction on ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig

Induction of Polyploidy in Giant Miscanthus (Miscanthus × Giganteus Greef Et Deu.)

Onion (Allium Cepa L.) as a Test Plant

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