Screening of nutritional and genetic anemias using elastic light scattering

Tong, Lieshu; Kauer, Josef; Chen, Xi; Chu, Kaiqin; Dou, Hu; Smith, Zachary J.

doi:10.1039/c8lc00377g

Cited by 3 publications

(1 citation statement)

References 50 publications

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“…In the current study, a 1-month iron supplementation program with a minimum escalation in hemoglobin contents of 2.00 g/dL was considered successful (42). When hemoglobin levels do not rise as expected and iron supplementation is continued, participants with anemia may be at an elevated risk of an oxidation reaction attributed to the prevalence of non-transferrin-bound iron that may act as free radicals in the body (43)(44)(45). Furthermore, there is also an enhanced risk of infection due to the pathogenicity of microorganisms in the presence of free iron (46).…”

Section: Discussionmentioning

confidence: 99%

Adherence Does Not Guarantee the Outcome of Iron Supplementation for Reproductive-Age Women With Anemia in West Papua Province, Indonesia: A Quasi-experimental Study

Rakanita

Sinuraya

Alfian

et al. 2023

International Journal of Women’s Health and Reproduction Scienc

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Objectives: Monitoring iron supplementation effectiveness in the affected populations is important in assuring its success. The research objective of this study is to evaluate the effectiveness of iron supplementation and its related factors in increasing the hemoglobin levels of women with anemia that reside in the area of Teluk Bintuni Regency in West Papua Province, Indonesia. Materials and Methods: A quasi-experimental study was performed to determine the changes in hemoglobin levels and the adherence between two supplementation groups (before and after supplementation). From the initial screening of hemoglobin levels of 875 reproductive-age women, 110 women with moderate and severe anemia were enrolled for a month-long iron supplementation therapy. This study was conducted from September 2018 until November 2019 at Teluk Bintuni Regency, West Papua, Indonesia. The changes in hemoglobin levels were measured after 30 days of iron supplementation. The associated factors, including participants’ characteristics, chronic energy deficiency (CED) levels, adherence to supplementation programs, and knowledge of anemia, were also assessed. The adherence level to the supplementation was measured using the Medication Adherence Rating Scale (MARS) questionnaires and the pill counting method. Results: The mean hemoglobin level significantly increased from 9.12±1.70 before supplementation to 10.15±1.65 after 30 days (P<0.001). Interestingly, results from the MARS questionnaires and pill counting method suggested that only 76% and 66% of participants adhered to the supplementation program, respectively. Further univariate analysis showed that adherence, ethnicity, and type of supplementation were factors that may influence the success of the iron supplementation therapy. Conclusions: Based on this study findings, it can be concluded that anemia is related to various factors, and its implementation should be carefully monitored, not solely depending on individual adherence.

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Section: Discussionmentioning

confidence: 99%

Adherence Does Not Guarantee the Outcome of Iron Supplementation for Reproductive-Age Women With Anemia in West Papua Province, Indonesia: A Quasi-experimental Study

Rakanita

Sinuraya

Alfian

et al. 2023

International Journal of Women’s Health and Reproduction Scienc

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Nanometer precise red blood cell sizing using a cost-effective quantitative dark field imaging system

Chen

Luo

et al. 2020

Biomed. Opt. Express

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Because of the bulk, complexity, calibration requirements, and need for operator training, most current flow-based blood counting devices are not appropriate for field use. Standard imaging methods could be much more compact, inexpensive, and with minimal calibration requirements. However, due to the diffraction limit, imaging lacks the nanometer precision required to measure red blood cell volumes. To address this challenge, we utilize Mie scattering, which can measure nanometer-scale morphological information from cells, in a dark-field imaging geometry. The approach consists of a custom-built dark-field scattering microscope with symmetrically oblique illumination at a precisely defined angle to record wide-field images of diluted and sphered blood samples. Scattering intensities of each cell under three wavelengths are obtained by segmenting images via digital image processing. These scattering intensities are then used to determine size and hemoglobin information via Mie theory and machine learning. Validation on 90 clinical blood samples confirmed the ability to obtain mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and red cell distribution width (RDW) with high accuracy. Simulations based on historical data suggest that an instrument with the accuracy achieved in this study could be used for widespread anemia screening.

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A method for scattering angle calibration in the rainbow region using a droplet stream

Li¹,

Huang²,

Fan³

et al. 2022

Opt. Express

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Accurate quantification of scattering angle versus detector pixel strongly determines the measurement accuracy of rainbow refractometry. This is an emerging measurement technique operating at backscatter angles and characterizing droplets or complex droplets in terms of size and refractive index. A novel method for calibration of the rainbow scattering angle using a monodisperse droplet stream is introduced and the achievable accuracy is estimated. The assumption of a linear pixel-to-angle relation is derived, and a calibration procedure is proposed based on global fit of calibration data to the theoretically known rainbow signal. The accuracy of this method was examined by simulations and experiments, where the uncertainties of a priori parameters of droplets were also considered and validated using shadowgraphy as a ground truth. The results confirm the feasibility of this method with a maximum absolute error of 0.032°and 3.9E-5°/pixel respectively for the intercept and slope of the linear relationship. These values translate into maximum uncertainties in diameter and refractive index of approx. 0.67% and 2.8 × 10−4.

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Screening of nutritional and genetic anemias using elastic light scattering

Abstract: Elastic light scattering and machine learning accurately discriminates between healthy children, those with iron deficiency, and those with thalassemia minor.

Cited by 3 publications

References 50 publications

Adherence Does Not Guarantee the Outcome of Iron Supplementation for Reproductive-Age Women With Anemia in West Papua Province, Indonesia: A Quasi-experimental Study

Adherence Does Not Guarantee the Outcome of Iron Supplementation for Reproductive-Age Women With Anemia in West Papua Province, Indonesia: A Quasi-experimental Study

Nanometer precise red blood cell sizing using a cost-effective quantitative dark field imaging system

A method for scattering angle calibration in the rainbow region using a droplet stream

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