Screening of pea germplasm for resistance to powdery mildew

Rana, Jai Chand; Banyal, D. K.; Sharma, Kavita; Sharma, Manish Kumar; Gupta, S. K.; Yadav, Satish Kumar

doi:10.1007/s10681-012-0798-6

Cited by 29 publications

(23 citation statements)

References 29 publications

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“…Ten days after inoculation, disease severity was rated based on a scale of 0–4 as described above. Plants with scores of 0–2 and 3–4 were classified as resistant and susceptible, respectively [25,26,27,31,58]. Those plants identified as E. pisi -resistant were tested again to confirm their resistance.…”

Section: Methodsmentioning

confidence: 99%

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Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms

Sun

Deng

Duan

et al. 2019

IJMS

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Powdery mildew caused by Erysiphe pisi DC. severely affects pea crops worldwide. The use of resistant cultivars containing the er1 gene is the most effective way to control this disease. The objectives of this study were to reveal er1 alleles contained in 55 E. pisi-resistant pea germplasms and to develop the functional markers of novel alleles. Sequences of 10 homologous PsMLO1 cDNA clones from each germplasm accession were used to determine their er1 alleles. The frame shift mutations and various alternative splicing patterns were observed during transcription of the er1 gene. Two novel er1 alleles, er1-8 and er1-9, were discovered in the germplasm accessions G0004839 and G0004400, respectively, and four known er1 alleles were identified in 53 other accessions. One mutation in G0004839 was characterized by a 3-bp (GTG) deletion of the wild-type PsMLO1 cDNA, resulting in a missing valine at position 447 of the PsMLO1 protein sequence. Another mutation in G0004400 was caused by a 1-bp (T) deletion of the wild-type PsMLO1 cDNA sequence, resulting in a serine to leucine change of the PsMLO1 protein sequence. The er1-8 and er1-9 alleles were verified using resistance inheritance analysis and genetic mapping with respectively derived F2 and F2:3 populations. Finally, co-dominant functional markers specific to er1-8 and er1-9 were developed and validated in populations and pea germplasms. These results improve our understanding of E. pisi resistance in pea germplasms worldwide and provide powerful tools for marker-assisted selection in pea breeding.

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Section: Methodsmentioning

confidence: 99%

“…The F 2:3 families with scores of 0–2 and 3–4 were classified as homozygous resistant and homozygous susceptible, respectively. Families with scores of 0–2 and 3–4 were considered segregated to E. pisi resistance [27,31,58]. The families identified as homozygous resistant or resistance segregated were subjected to repeated testing.…”

Section: Methodsmentioning

confidence: 99%

Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms

Sun

Deng

Duan

et al. 2019

IJMS

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“…Till date, various research work has been carried out for the management of powdery mildew in pea plant [9] [10] [11]. Knowing the importance of this vegetable crop by exploring the literature we therefore, continued this research by deciphering the diversity at er loci in pathogen resistant pea lines [12] which was screened under invitro conditions [13]. Pathogen causing powdery mildew was collected from Northwest Himalayas in which the maximum number of isolates were collected from Lahul area of trans-Himalayas [14] [15].…”

Section: Discussionmentioning

confidence: 99%

Deciphering Diversity at er Loci for Diversification of Powdery Mildew Resistance in Pea

Banyal

Dixit

Chaudhary

et al. 2022

Preprint

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Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the world’s population by improving their agronomic traits using various biotechnological tools. Pea- an important cash crop, rich in nutrients, but frequently infected with powdery mildew (fungal disease) that destroy the whole crop which causes the economy loss for growers. We therefore, targeted this research to find the pathogen resistant pea lines and further decipher the diversity at er locus among resistant pea lines. Screening for resistant pea lines was done with Erysiphe pisi isolates (Genebank submission: KX45 5922.1) under net house and green house conditions. Molecular studies revealed that Erysiphe resistant (er1) gene was present in 40 lines out of selected 50 pea lines and the mutational character was conferred up to 36 genotypes with 11 haplotype groups. The haplotype (gene) diversity (Hd) was found to be 0.5571±0.099 SD and the nucleotide diversity (Pi) was 0.0160±0.0042 SD Majority of resistant lines (67%) occurred in Hap-1, other remaining haplotypes (Hap 2-10) having 33% resistant lines, each showing characteristic nucleotide substitutions with respect to reference PsMLO1 gene; genotypes from these divergent haplotypes can be used in pea resistance breeding to avoid genetic homogeneity and genetic vulnerability.

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“…Longwan 1. Ten days after inoculation, disease severity was scored using a scale of 0–4, according to the infected foliage area, macroscopic and microscopic observation of mycelia growth and sporulation [ 29 , 39 , 40 , 43 ] ( S1 Fig ). 0 = No colony or mycelium growth on inoculated leave; 1 = few colony with a film of mycelium growth and mycelium with very little sporulation; 2 = a few countable colonies with evident mycelium growth and mycelium with slight sporulation; 3 = uncountable colonies with abundant growth mycelium and mycelium with moderate to heavy sporulation; 4 = infected leaves fully covered by abundant growth mycelium with heavy to very heavy sporulation.…”

Section: Methodsmentioning

confidence: 99%

“…The disease severity was determined using a scale of 0–4 as stated above ( S1 Fig ), 10 days after inoculation. Plants with scores of 0–2 and 3–4 were classified as resistant and susceptible, respectively [ 29 , 43 ]. The plants considered resistant to E .…”

Section: Methodsmentioning

confidence: 99%

Discovery of a Novel er1 Allele Conferring Powdery Mildew Resistance in Chinese Pea (Pisum sativum L.) Landraces

Sun

Wang

et al. 2016

PLoS ONE

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Pea powdery mildew, caused by Erysiphe pisi D.C., is an important disease worldwide. Deployment of resistant varieties is the main way to control this disease. This study aimed to screen Chinese pea (Pisum sativum L.) landraces resistant to E. pisi, and to characterize the resistance gene(s) at the er1 locus in the resistant landraces, and to develop functional marker(s) specific to the novel er1 allele. The 322 landraces showed different resistance levels. Among them, 12 (3.73%), 4 (1.24%) and 17 (5.28%) landraces showed immunity, high resistance and resistance to E. pisi, respectively. The other landraces appeared susceptible or highly susceptible to E. pisi. Most of the immune and highly resistant landraces were collected from Yunnan province. To characterize the resistance gene at the er1 locus, cDNA sequences of PsMLO1 gene were determined in 12 immune and four highly resistant accessions. The cDNAs of PsMLO1 from the immune landrace G0005576 produced three distinct transcripts, characterized by a 129-bp deletion, and 155-bp and 220-bp insertions, which were consistent with those of er1-2 allele. The PsMLO1 cDNAs in the other 15 resistant landraces produced identical transcripts, which had a new point mutation (T→C) at position 1121 of PsMLO1, indicating a novel er1 allele, designated as er1-6. This mutation caused a leucine to proline change in the amino acid sequence. Subsequently, the resistance allele er1-6 in landrace G0001778 was confirmed by resistance inheritance analysis and genetic mapping on the region of the er1 locus using populations derived from G0001778 × Bawan 6. Finally, a functional marker specific to er1-6, SNP1121, was developed using the high-resolution melting technique, which could be used in pea breeding via marker-assisted selection. The results described here provide valuable genetic information for Chinese pea landraces and a powerful tool for pea breeders.

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Screening of pea germplasm for resistance to powdery mildew

Cited by 29 publications

References 29 publications

Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms

Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms

Deciphering Diversity at er Loci for Diversification of Powdery Mildew Resistance in Pea

Discovery of a Novel er1 Allele Conferring Powdery Mildew Resistance in Chinese Pea (Pisum sativum L.) Landraces

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