Screening of peptide ligands that bind to the Fc region of IgG using peptide array and its application to affinity purification of antibody

Sugita, Tadaaki; Katayama, Makoto; Okochi, Mina; Kato, Ryuji; Ichihara, Takamitsu; Honda, Hiroyuki

doi:10.1016/j.bej.2013.06.017

Cited by 47 publications

(18 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sugita and coworkers have reported two high affinity octameric peptides towards the Fc-region of IgG [ 61 ]. In their report, peptides derived from the outer membrane amino acid sequence of the IgG Fcγ receptor, one of the well-known IgG Fc-binding proteins, were screened.…”

Section: Immunoglobulin Binding Peptides and Peptidomimeticsmentioning

confidence: 99%

Fc-Binding Ligands of Immunoglobulin G: An Overview of High Affinity Proteins and Peptides

2016

View full text Add to dashboard Cite

The rapidly increasing application of antibodies has inspired the development of several novel methods to isolate and target antibodies using smart biomaterials that mimic the binding of Fc-receptors to antibodies. The Fc-binding domain of antibodies is the primary binding site for e.g., effector proteins and secondary antibodies, whereas antigens bind to the Fab region. Protein A, G, and L, surface proteins expressed by pathogenic bacteria, are well known to bind immunoglobulin and have been widely exploited in antibody purification strategies. Several difficulties are encountered when bacterial proteins are used in antibody research and application. One of the major obstacles hampering the use of bacterial proteins is sample contamination with trace amounts of these proteins, which can invoke an immune response in the host. Many research groups actively develop synthetic ligands that are able to selectively and strongly bind to antibodies. Among the reported ligands, peptides that bind to the Fc-domain of antibodies are attractive tools in antibody research. Besides their use as high affinity ligands in antibody purification chromatography, Fc-binding peptides are applied e.g., to localize antibodies on nanomaterials and to increase the half-life of proteins in serum. In this review, recent developments of Fc-binding peptides are presented and their binding characteristics and diverse applications are discussed.

show abstract

Section: Immunoglobulin Binding Peptides and Peptidomimeticsmentioning

confidence: 99%

Fc-Binding Ligands of Immunoglobulin G: An Overview of High Affinity Proteins and Peptides

2016

View full text Add to dashboard Cite

show abstract

“…Peptide array is the one of the most useful tools for analyzing various protein-protein interactions (e.g., antibody 20 , receptor 21 , and cytokine 22 ). We have used a cellulose membrane-based peptide array to identify various peptides, including IgG Fc-binding peptide 23 , cell death-inducible peptide 24 , bile acid-binding peptide 25 , and cell-adhesive peptides 26 . Moreover, we have constructed a photo-cleavable peptide array for designing a solubilized peptide library, but not the immobilized library.…”

mentioning

confidence: 99%

Effects of the properties of short peptides conjugated with cell-penetrating peptides on their internalization into cells

Matsumoto

Okochi

Shimizu

et al. 2015

Sci Rep

Self Cite

View full text Add to dashboard Cite

Peptides, especially intracellular functional peptides that can play a particular role inside a cell, have attracted attention as promising materials to control cell fate. However, hydrophilic materials like peptides are difficult for cells to internalize. Therefore, the screening and design of intracellular functional peptides are more difficult than that of extracellular ones. An effective high-throughput screening system for intracellular functional peptides has not been reported. Here, we demonstrate a novel peptide array system for screening intracellular functional peptides, in which both cell-penetrating peptide (CPP) domain and photo-cleavable linkers are used. By using this screening system, we determined how the cellular uptake properties of CPP-conjugated peptides varied depending on the properties of the conjugated peptides. We found that the internalization ability of CPP-conjugated peptides varied greatly depending on the property of the conjugated peptides, and anionic peptides drastically decreased the uptake ability. We summarized our data in a scatter diagram that plots hydrophobicity versus isoelectric point (pI) of conjugated peptides. These results define a peptide library suitable for screening of intracellular functional peptides. Thus, our system, including the diagram, is a promising tool for searching biological active molecules such as peptide-based drugs.

show abstract

“…The additional advantage of using these peptides to immobilize antibodies rather than proteins, such as protein A or G, is their small size that provides better control in grafting the molecule on the surface and preventing steric hindrance. These synthesized peptides have been designed and used for antibody purification purposes, 28,29 and to fabricate biosensors chips. 30 To our knowledge, no studies have tested the efficiency of peptide-based antibody immobilization to capture circulating cells.…”

Section: Introductionmentioning

confidence: 99%

Surface grafting of Fc-binding peptides as a simple platform to immobilize and identify antibodies that selectively capture circulating endothelial progenitor cells

et al. 2020

View full text Add to dashboard Cite

show abstract

Screening of peptide ligands that bind to the Fc region of IgG using peptide array and its application to affinity purification of antibody

Cited by 47 publications

References 34 publications

Fc-Binding Ligands of Immunoglobulin G: An Overview of High Affinity Proteins and Peptides

Fc-Binding Ligands of Immunoglobulin G: An Overview of High Affinity Proteins and Peptides

Effects of the properties of short peptides conjugated with cell-penetrating peptides on their internalization into cells

Surface grafting of Fc-binding peptides as a simple platform to immobilize and identify antibodies that selectively capture circulating endothelial progenitor cells

Contact Info

Product

Resources

About