Screening of perhydrolases to optimize glucose oxidase-perhydrolase-in situ chemical oxidation cascade reaction system and its application in melanin decolorization

Jia, Wenjing; Li, Huan; Wang, Qian; Zheng, Kuo; Lin, Hong; Li, Xin; Huang, Jianzhong; Xu, Linting; Dong, Wanqian; Shu, Zhengyu

doi:10.1016/j.jbiotec.2021.01.013

Cited by 6 publications

(2 citation statements)

References 43 publications

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“…Melanin production in fungi is associated with increased survival in stress conditions, virulence, dissemination, and host immune response ( Eisenman and Casadevall 2012 ). Exophiala dermatitidis produce DHN-melanin from 1,8 dihydroxynaphthalene polymerization, the more common and better-characterized pathway in fungi, compared to L-DOPA, produced DOPA-melanin, or pyomelanin produced from the degradation of tyrosine ( Jia et al 2021 ). DHN synthesis initiates from endogenous acetyl CoA or melanyl CoA, which is converted to 1,3,6,8 tetrahydroxynaphthalene by polyketide synthase, and converted to DHN-melanin through a series of reductions and polymerizations.…”

Section: Discussionmentioning

confidence: 99%

In host evolution of Exophiala dermatitidis in cystic fibrosis lung micro-environment

Kurbessoian

Murante

Crocker

et al. 2023

G3: Genes, Genomes, Genetics

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Individuals with cystic fibrosis (CF) are susceptible to chronic lung infections that lead to inflammation and irreversible lung damage. While most respiratory infections that occur in CF are caused by bacteria, some are dominated by fungi such as the slow-growing black yeast Exophiala dermatitidis. Here, we analyze isolates of E. dermatitidis cultured from two samples, collected from a single subject two years apart. One isolate genome was sequenced using long-read Nanopore technology as an in-population reference to use in comparative single nucleotide polymorphism (SNP) and insertion-deletion (INDEL) variant analyses of twenty-three isolates. We then used population genomics and phylo-genomics to compare the isolates to each other as well as the reference genome strain E. dermatitidis NIH/UT8656. Within the CF lung population, three E. dermatitidis clades were detected, each with varying mutation rates. Overall, the isolates were highly similar suggesting that they were recently diverged. All isolates were MAT 1-1, which was consistent with their high relatedness and the absence of evidence for mating or recombination between isolates. Phylogenetic analysis grouped sets of isolates into clades that contained isolates from both early and late time points indicating there are multiple persistent lineages. Functional assessment of variants unique to each clade identified alleles in genes that encode transporters, cytochrome P450 oxidoreductases, iron acquisition, and DNA repair processes. Consistent with the genomic heterogeneity, isolates showed some stable phenotype heterogeneity in melanin production, subtle differences in antifungal minimum inhibitory concentrations, and growth on different substrates. The persistent population heterogeneity identified in lung-derived isolates is an important factor to consider in the study of chronic fungal infections, and the analysis of changes in fungal pathogens over time may provide important insights into the physiology of black yeasts and other slow-growing fungi in vivo.

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Section: Discussionmentioning

confidence: 99%

In host evolution of Exophiala dermatitidis in cystic fibrosis lung micro-environment

Kurbessoian

Murante

Crocker

et al. 2023

G3: Genes, Genomes, Genetics

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“…Oxidative enzymes that are able to degrade melanin, such as peroxidase (EC 1.11.1.7) and laccase (EC 1.10.3.2), have received great interest as a topical alternative to common tyrosinase inhibitors [ 14 , 15 ]. The use of peroxidase requires H 2 O 2 as a primary oxidant, which favors skin irritation [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ]. On the contrary, laccase is able to activate dioxygen as a primary oxidant without the necessity for peroxide intermediates [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Laccase Mediator Cocktail System as a Sustainable Skin Whitening Agent for Deep Eumelanin Decolorization

Gigli

Piccinino

Avitabile

et al. 2022

IJMS

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The overproduction of eumelanin leads to a panel of unaesthetic hyper-pigmented skin diseases, including melasma and age spots. The treatment of these diseases often requires the use of tyrosinase inhibitors, which act as skin whitening agents by inhibiting the synthesis of eumelanin, with harmful side effects. We report here that laccase from Trametes versicolor in association with a cocktail of natural phenol redox mediators efficiently degraded eumelanin from Sepia officinalis, offering an alternative procedure to traditional whitening agents. Redox mediators showed a synergistic effect with respect to their single-mediator counterpart, highlighting the beneficial role of the cocktail system. The pro-oxidant DHICA sub-units of eumelanin were degraded better than the DHI counterpart, as monitored by the formation of pyrrole-2,3,5-tricarboxylic acid (PTCA) and pyrrole-2,3-dicarboxylic acid (PDCA) degradation products. The most effective laccase-mediated cocktail system was successively applied in a two-component prototype of a topical whitening cream, showing high degradative efficacy against eumelanin.

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Mycobacterium smegmatis acyltransferase: The big new player in biocatalysis

Cannazza

Donzella

Pellis

et al. 2022

Biotechnology Advances

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Screening of perhydrolases to optimize glucose oxidase-perhydrolase-in situ chemical oxidation cascade reaction system and its application in melanin decolorization

Cited by 6 publications

References 43 publications

In host evolution of Exophiala dermatitidis in cystic fibrosis lung micro-environment

In host evolution of Exophiala dermatitidis in cystic fibrosis lung micro-environment

Laccase Mediator Cocktail System as a Sustainable Skin Whitening Agent for Deep Eumelanin Decolorization

Mycobacterium smegmatis acyltransferase: The big new player in biocatalysis

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