Screening of permeable compounds in Flos Lonicerae Japonicae with liposome using ultrafiltration and HPLC

Chen, Xiaoping; Xia, Yuqin; Lu, Ye; Liang, Jianying

doi:10.1016/j.jpba.2010.08.028

Cited by 28 publications

(13 citation statements)

References 12 publications

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“…According to MS spectra of the product ions in compounds 5, 6, 8, and 9, the [M-15-H] − ion observed corresponded to the neutral loss of CH 3 caused by the cleavage of methoxyl from the [M-H] − . Based on the differences existed in the molecular weight, these compounds were characterized as tectorigenin (5), biochanin A (6), 3 -methoxy daidzein (8), and irisolidone (9) [22,23]. Compound 7 exhibited [M-H] − ion at m/z 269 and it was characterized as genistein (7) by comparing , and irisolidone (97.54%).…”

Section: Identification Of Bound Ligandsmentioning

confidence: 99%

“…This method could retain suspended solids and compounds of high molecular weight, while the liquid and low molecular weight compounds are allowed to pass through membrane depending on the nominal molecular weight cut-off of membrane. Based on these features, active compounds bound to protein could be retained by membrane together with protein after the incubation of natural product extract and protein [8]. Through high performance liquid chromatography-mass spectrometry (HPLC-MS) analysis, the structure of active compounds could be identified.…”

Section: Introductionmentioning

confidence: 99%

“…Through high performance liquid chromatography-mass spectrometry (HPLC-MS) analysis, the structure of active compounds could be identified. Therefore, due to its time saving, easy operation, and high reliability, ultrafiltration was successfully applied in analysis of active compounds from complex mixtures binding with biomacromolecules like liposome, rat plasma protein, and enzymes [8,9].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of Bovine Serum Albumin Ligands fromPuerariae flosUsing Ultrafiltration Combined with HPLC-MS

Tang

Liu

2015

Journal of Chemistry

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Rapid screening techniques for identification of active compounds from natural products are important not only for clarification of the therapeutic material basis, but also for supplying suitable chemical markers for quality control. In the present study, ultrafiltration combined with high performance liquid chromatography-mass spectrometry (HPLC-MS) was developed and conducted to screen and identify bovine serum albumin (BSA) bound ligands from Puerariae flos. Fundamental parameters affecting the screening like incubation time, BSA concentration, pH, and temperature were studied and optimized. Under the optimum conditions, nine active compounds were identified by UV and MS data. The results indicated that this method was able to screen and identify BSA bound ligands form natural products without the need of preparative isolation techniques. Moreover, the method has more effective with easier operation procedures.

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Section: Identification Of Bound Ligandsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Bovine Serum Albumin Ligands fromPuerariae flosUsing Ultrafiltration Combined with HPLC-MS

Tang

Liu

2015

Journal of Chemistry

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“…Thus, CU became a useful technique for screening active compounds bound to biomacromolecules such as bovine serum albumin, 5 α-glucosidase, 6 quinone reductase-2, 7 deoxyribonucleic acid (DNA) and liposomes. 8,9 High performance liquid chromatography-mass spectrometry (HPLC-MS or LC-MS) has been widely applied for the simultaneous separation and identification of active compounds in complex mixtures. 10 The combination of CU and LC-MS (CU-LC-MS) became a powerful tool in analyzing active compounds due to its simple operation, high speed and low sample consumption.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Xanthine Oxidase Inhibitors fromPuerariae flosUsing Centrifugal Ultrafiltration Coupled with HPLC-MS

Liu¹,

Xiao²,

Ma³

et al. 2016

Journal of the Brazilian Chemical Society

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In this study, centrifugal ultrafiltration coupled with high performance liquid chromatographymass spectrometry was utilized to screen and identify xanthine oxidase inhibitors from Puerariae flos extract. The experimental conditions of centrifugal ultrafiltration including xanthine oxidase concentration, incubation time, pH and temperature were optimized. At the optimum condition (xanthine oxidase concentration: 30.0 μg mL -1 , incubation time: 20 min, pH 7.0 and temperature: 25 °C), four compounds were successfully screened from P. flos extract and identified as tectoridin, daidzin, ononin and biochanin A. The yields of tectoridin, daidzin, ononin and biochanin A were 0.231, 0.117, 0.303 and 0.089 g from 50.0 g crude P. flos samples. The inhibitory activities of these compounds were verified by xanthine oxidase inhibition assays. The experimental half maximal inhibitory concentration (IC 50 ) values of tectoridin, daidzin, ononin and biochanin A were 88.5, 85.1, 88.8 and 87.0 μmol L -1 , and the binding degree of them were 5. 70, 8.28, 6.31 and 37.83% at the optimum condition, respectively. The proposed method provided a rapid and effective way to screen and analyze active compounds from natural products. Keywords: HPLC-MS, inhibitor, Puerariae flos, ultrafiltration, xanthine oxidase IntroductionNatural products have been used as the most consistently successful resources of new drug discovery for a long time because of their great diversity of the chemical structures and better drug-like properties compared to the synthetic compounds.1 However, natural products resources like plant extracts were very complex and usually contained various kinds of components. Separation and purification of natural compounds were time-consuming and laborious processes.2 Hence, simple and effective methods aiming at directly screening natural product extracts would be greatly helpful for drug discovery. Centrifugal ultrafiltration (CU) utilized centrifugal force and a semi-permeable membrane to retain suspended solids and high molecular weight solutes, while liquid and low molecular weight solutes were allowed to pass through depending on the nominal molecular weight cut-off of the membrane. 4 Based on these features, active compounds could be retained by membrane together with enzyme due to the binding with enzyme. Thus, CU became a useful technique for screening active compounds bound to biomacromolecules such as bovine serum albumin, 5 α-glucosidase, 6 quinone reductase-2, 7 deoxyribonucleic acid (DNA) and liposomes. 8,9 High performance liquid chromatography-mass spectrometry (HPLC-MS or LC-MS) has been widely applied for the simultaneous separation and identification of active compounds in complex mixtures. 10The combination of CU and LC-MS (CU-LC-MS) became a powerful tool in analyzing active compounds due to its simple operation, high speed and low sample consumption. Compared with immobilized enzyme screening assay, complex synthesis procedures could be avoided in CU-LC-MS as well.11 Moreover, the efficient screeni...

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“…Currently, FL is used as the main raw materials for the production of some herb teas greatly needed in the Chinese market, such as Wanglaoji, Heqizheng and Jiaduobao. So far many studies have focused on the extracts of FL by alcohol solution (Chen et al, 2011;Zhang et al, 2013). It is reported that hyperoside, chlorogenic acid and caffeic acid are the main constituents of FL (Kakuda et al, 2000;Peng et al, 2000), which can be used to scavenge free radicals and have anti-inflammatory activity (Leung et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Variation of essential oil content and antioxidant activity of Lonicera species in different sites of China

Zhang

Yao

Duan

et al. 2015

Industrial Crops and Products

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Screening of permeable compounds in Flos Lonicerae Japonicae with liposome using ultrafiltration and HPLC

Cited by 28 publications

References 12 publications

Analysis of Bovine Serum Albumin Ligands fromPuerariae flosUsing Ultrafiltration Combined with HPLC-MS

Analysis of Bovine Serum Albumin Ligands fromPuerariae flosUsing Ultrafiltration Combined with HPLC-MS

Analysis of Xanthine Oxidase Inhibitors fromPuerariae flosUsing Centrifugal Ultrafiltration Coupled with HPLC-MS

Variation of essential oil content and antioxidant activity of Lonicera species in different sites of China

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