Screening of plants found in the State of Amazonas, Brazil for activity against Aedes aegypti larvae

Pohlit, Adrian Martin; Quinard, Etienne Louis Jacques; Nunomura, Sergio Massayoshi; Tadei, Wanderli Pedro; Hidalgo, Ari de Freitas; Pinto, Ana Cristina da Silva; Santos, Elba Vieira Mustafa dos; Morais, Sabrina Kelly Reis de; Saraiva, Rita; Ming, Lin Chau; Alecrim, Alexandre Mascarenhas; Ferraz, Alfeu de Barros; Pedroso, Andreza Cristiana da Silva; Diniz, Elisangela Vieira; Finney, Ellen Kathryn; Gomes, Erika de Oliveira; Dias, Hercules Bezerra; Souza, Katiúscia dos Santos de; Oliveira, Lívia Costa de; Don, Luciana de Castro; Queiroz, Maria Mireide Andrade; Henrique, Marycleuma Campos; Santos, Mírian dos; Júnior, Orivaldo Da Silva Lacerda; Pinto, Patrícia de Souza; Silva, Suniá Gomes; Graça, Yara Rodrigues

doi:10.1590/s0044-59672004000100012

Cited by 46 publications

(29 citation statements)

References 20 publications

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“…aegypti of extracts from leaves, stems, and roots of leguminous species prepared with organic solvents (Pohlit et al 2004, Luna et al 2005, Omena et al 2007). Nevertheless, little attention has been addressed to the potential of seeds as sources of secondary compounds with larvicidal properties (Jang et al 2002, Luna et al 2005.…”

Section: Resultsmentioning

confidence: 99%

Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L.

Farias

Cavalheiro

Viana

et al. 2010

An. Acad. Bras. Ciênc.

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This study assessed the toxicity of seed water extracts of 15 leguminous species upon Aedes aegypti larvae. A partial chemical and biochemical characterization of water extracts, as well as the assessment of their acute toxicity in mice, were performed. The extracts of Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum and Piptadenia moniliformis caused 100% of mortalit y after 1 to 3 h of exposure. They showed LC 50 and LC 90 values ranging from 0.43 ± 0.01 to 9.06 ± 0.12 mg/mL and from 0.71 ± 0.02 to 13.03 ± 0.15 mg/mL, respectively. Among the secondary metabolite constituents, the seed water extracts showed tannins, phenols, flavones, favonols, xanthones, saponins and alkaloids. The extracts also showed high soluble proteins content (0.98 to 7.71 mg/mL), lectin (32 to 256 HU/mL) and trypsin inhibitory activity (3.64 ± 0.43 to 26.19 ± 0.05 gIT/kg of flour) The electrophoretic profiles showed a great diversity of protein bands, many of which already described as insecticide proteins. The extracts showed low toxicity to mice (LD 50 > 0.15 ± 0.01 g/kg body weight), but despite these promising results, further studies are necessary to understand the toxicity of these extracts and their constituents from primary and secondary metabolism upon Ae. aegypti.

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Section: Resultsmentioning

confidence: 99%

Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L.

Farias

Cavalheiro

Viana

et al. 2010

An. Acad. Bras. Ciênc.

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“…One specific insecticidal phenylpropanoid is dimethoxy-4,5-methylenedioxy-allylbenzene (dillapiol) (Gottlieb et al, 1981). Extracts from Piper aduncum, known in Brazil as pimenta longa (long pepper), are larvicidal (Bernard et al, 1995;Pohlit et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Potential control of Aedes aegypti (Diptera: Culicidae) with Piper aduncum L. (Piperaceae) extracts demonstrated by chromosomal biomarkers and toxic effects on interphase nuclei

Wj²,

Jj³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. Dillapiol, a phenylpropanoid isolate from essential oils of leaves of Piper aduncum (Piperaceae), has insecticidal, fungicidal and antimicrobial activities. The insecticidal activity of dil- Genotoxic effect of dillapiol on dengue mosquitoes lapiol was tested in vivo on the larvae and pupae of Aedes aegypti, the mosquito vector of dengue. Specifically, the effect of dillapiol on the formation of micronuclei and chromosome aberrations was analyzed. Dillapiol treatments comprised two concentrations of 200 and 400 μg/mL, dissolved in well water, and a pure well water control used to rear four generations of mosquitoes. Micronuclei occurred in mitotic diploid and tetraploid chromosomes of larvae; nuclear abnormalities also occurred in interphase, metaphase, telophase, and single nucleus cells of pupae. Mortality, oviposition, chromosome breakage, and anaphase bridges were significantly greater in the extract treatments than in controls. The genotoxic effects of dillapiol described here suggest that this natural product may be a useful alternative for the control of A. aegypti.

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“…Em busca de novas alternativas para o controle do Aedes aegypti, várias pesquisas vêm sendo desenvolvidas no intuito de encontrar um inseticida eficaz com menor impacto ambiental [11][12][13][14][15][16][17][18][19] .…”

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Alterações ultraestruturais em larvas de Aedes aegypti submetidas ao diterpeno labdano, isolado de Copaifera reticulata (Leguminosae), e à uma fração rica em taninos de Magonia pubescens (Sapindaceae)

Valotto

Silva

Cavasin

et al. 2011

Rev. Soc. Bras. Med. Trop.

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INTRODUÇÃO: Dengue é um importante problema de saúde pública, em vários países, e tem como principal vetor o Aedes aegypti, mosquito mais adaptado às áreas urbanizadas. Apresenta-se, pela primeira vez, as alterações ultraestruturais em larvas de 3º estádio, desse mosquito, causadas pelos larvicidas naturais, um diterpeno labdano, extraído de Copaifera reticulata, e uma fração rica em taninos catéquicos, extraída de Magonia pubescens, evidenciando o mecanismo de ação dessas substâncias. MÉTODOS: Os experimentos foram realizados com larvas de 3º estádio em solução de 0,9ppm, do diterpeno (3-β-acetoxylabdan-8(17)-13-dien-15-óico) e de 3,7ppm, da fração majoritária de tanino catéquico de massa molecular 864Da. Obtiveram-se as substâncias através de fracionamentos cromatográficos sucessivos, identificadas por ressonância magnética nuclear de hidrogênio e espectrometria de massas. As larvas que atingiram estado letárgico foram coletadas e dissecadas e seus tubos digestórios fixados, desidratados, emblocados e polimerizados. Cortes ultrafinos foram feitos e contrastados com acetato de uranila 3% e citrato de chumbo, posteriormente, levados ao microscópio eletrônico. RESULTADOS: As principais alterações ultraestruturais provocadas pelos diterpeno e tanino sobre larvas de Aedes aegypti foram vacuolização citoplasmática, desorganização e degeneração celular, mudança estrutural dos microvilos e deslocamento das células da lâmina basal. CONCLUSÕES: O diterpeno e a fração rica em taninos catéquicos provocaram a morte das larvas de Aedes aegypti através da destruição celular no intestino médio.

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Screening of plants found in the State of Amazonas, Brazil for activity against Aedes aegypti larvae

Cited by 46 publications

References 20 publications

Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L.

Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L.

Potential control of Aedes aegypti (Diptera: Culicidae) with Piper aduncum L. (Piperaceae) extracts demonstrated by chromosomal biomarkers and toxic effects on interphase nuclei

Alterações ultraestruturais em larvas de Aedes aegypti submetidas ao diterpeno labdano, isolado de Copaifera reticulata (Leguminosae), e à uma fração rica em taninos de Magonia pubescens (Sapindaceae)

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