Screening Of Plastic Degrading Bacteria from Dumped Soil Area

Jumaah, Omar Saad

doi:10.9790/2402-1105029398

Cited by 30 publications

(14 citation statements)

References 7 publications

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“…[25,38], were only present in low abundances (less than 1% relative abundance). This is different from recent discoveries suggesting that the presence of plastics leads to an enrichment of microorganisms which possess plastic-degrading abilities [16,22]. An exciting example of such studies is the discovery that Ideonella sakaiensis obtained from plastic debris that can degrade polyethylenterephthalat (PET).…”

Section: Discussionmentioning

confidence: 85%

“…Besides ecological studies addressing the concept of a 'microbial plastisphere', the aim to discover microorganisms capable of degrading synthetic polymers has motivated a number of microbial studies in the last few years [15][16][17][18][19][20][21][22][23][24][25][26][27]. So far, microorganisms from more than 90 genera were found to possess plastic-degrading abilities [22]. Those findings created a scientific framework for future eco-remediation technologies.…”

Section: Introductionmentioning

confidence: 99%

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The Terrestrial Plastisphere: Diversity and Polymer-Colonizing Potential of Plastic-Associated Microbial Communities in Soil

et al. 2021

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The concept of a ‘plastisphere microbial community’ arose from research on aquatic plastic debris, while the effect of plastics on microbial communities in soils remains poorly understood. Therefore, we examined the inhabiting microbial communities of two plastic debris ecosystems with regard to their diversity and composition relative to plastic-free soils from the same area using 16S rRNA amplicon sequencing. Furthermore, we studied the plastic-colonizing potential of bacteria originating from both study sites as a measure of surface adhesion to UV-weathered polyethylene (PE) using high-magnification field emission scanning electron microscopy (FESEM). The high plastic content of the soils was associated with a reduced alpha diversity and a significantly different structure of the microbial communities. The presence of plastic debris in soils did not specifically enrich bacteria known to degrade plastic, as suggested by earlier studies, but rather shifted the microbial community towards highly abundant autotrophic bacteria potentially tolerant to hydrophobic environments and known to be important for biocrust formation. The bacterial inoculates from both sites formed dense biofilms on the surface and in micrometer-scale surface cracks of the UV-weathered PE chips after 100 days of in vitro incubation with visible threadlike EPS structures and cross-connections enabling surface adhesion. High-resolution FESEM imaging further indicates that the microbial colonization catalyzed some of the surface degradation of PE. In essence, this study suggests the concept of a ‘terrestrial plastisphere’ as a diverse consortium of microorganisms including autotrophs and other pioneering species paving the way for those members of the consortium that may eventually break down the plastic compounds.

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Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

The Terrestrial Plastisphere: Diversity and Polymer-Colonizing Potential of Plastic-Associated Microbial Communities in Soil

et al. 2021

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“…Over 90 microorganisms, including bacteria and fungi, have been reported to degrade petroleumplastics, (Jumaah 2017). Most studies of PE biodegradation have reported that bacteria, such as with separate standards and test methods (Harrison et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Microbial degradation of low-density polyethylene and synthesis of polyhydroxyalkanoate polymers

2019

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We have characterized the ability of eight bacterial strains to utilize powdered low-density polyethylene (LDPE) plastic (untreated and without any additives) as a sole carbon source. Cell mass production on LDPE-containing medium after 21 days of incubation varied between 0.083 ± 0.015 g/L cell dry mass (cdm) for Micrococcus luteus IRN20 and 0.39 ± 0.036 g/L for Cupriavidus necator H16. The percent decrease in LDPE mass ranged from 18.9% ± 0.72% for M. luteus IRN20 to 33.7% ± 1.2% for C. necator H16. Linear alkane hydrolysis products from LDPE degradation were detected in the culture media, and the carbon chain lengths of the hydrolysis products detected varied, depending on the species of bacteria. We also determined that C. necator H16 produced short-chain-length polyhydroxyalkanoate biopolymers, while Pseudomonas putida LS46 and Acinetobacter pittii IRN19 produced medium-chain-length biopolymers while growing on polyethylene powder. Cupriavidus necator H16 accumulated poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-V) polymers to 3.18% ± 0.4% of cdm. The monomer composition of the PHB-V was 94.9% ± 0.61% 3-hydroxybutyrate and 5.03% ± 0.56% 3-hydroxyvalerate. This is the first report that provides direct evidence for simultaneous bioconversion of LDPE plastic to biodegradable polyhydroxyalkanoate polymers.

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“…The data of altitude, longitude and latitude with GPS coordinates was given in the Table 1. Soil samples were collected from the marked areas (Plate 1) and analysed for their physicochemical properties (Jumaah, 2017).…”

Section: Samplingmentioning

confidence: 99%

Isolation and Screening of Plastic Degrading Bacteria from Dumping Sites of Solid Waste

Rana¹,

Rana²

2020

Int.J.Curr.Microbiol.App.Sci

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Screening Of Plastic Degrading Bacteria from Dumped Soil Area

Cited by 30 publications

References 7 publications

The Terrestrial Plastisphere: Diversity and Polymer-Colonizing Potential of Plastic-Associated Microbial Communities in Soil

The Terrestrial Plastisphere: Diversity and Polymer-Colonizing Potential of Plastic-Associated Microbial Communities in Soil

Microbial degradation of low-density polyethylene and synthesis of polyhydroxyalkanoate polymers

Isolation and Screening of Plastic Degrading Bacteria from Dumping Sites of Solid Waste

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