Screening of Polyethylene-Degrading Bacteria from Rhyzopertha Dominica and Evaluation of Its Key Enzymes Degrading Polyethylene

Zhang, Yao; Yuan, Lin; Gou, Hongmei; Feng, Xu; Zhang, Xian; Yang, Lijuan

doi:10.3390/polym14235127

Cited by 22 publications

(6 citation statements)

References 34 publications

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“…This might be due to the lignolytic enzymes produced by C. sphaerospermum. The results of the present study are in accord with the previous reports on polyethylene degradation by microbes 55 , 56 .…”

Section: Resultssupporting

confidence: 93%

Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere

Sathiyabama,

Boomija,

Sathiyamoorthy

et al. 2024

Sci Rep

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Plastic accumulation is a severe threat to the environment due to its resistivity to thermal, mechanical and biological processes. In recent years, microbial degradation of plastic waste disposal is of interest because of its eco-friendly nature. In this study, a total of 33 fungi were isolated from the plastisphere and out of which 28 fungal species showed halo zone of clearance in agarized LDPE media. The fungus showing highest zone of clearance was further used to evaluate its degradation potential. Based on morphological and molecular technique, the fungus was identified as Cladosporium sphaerospermum. The biodegradation of LDPE by C. sphaerospermum was evaluated by various methods. The exposure of LDPE with C. sphaerospermum resulted in weight loss (15.23%) in seven days, higher reduction rate (0.0224/day) and lower half-life (30.93 days). FTIR analysis showed changes in functional group and increased carbonyl index in LDPE treated with C. sphaerospermum. SEMimages evidenced the formation of pits, surface aberrations and grooves on the LDPE film treated with the fungus whereas the untreated control LDPE film showed no change. AFM analysis confirmed the surface changes and roughness in fungus treated LDPE film. This might be due to the extracellular lignolytic enzymes secreted by C. sphaerospermum grown on LDPE. The degradation of polyethylene by Short chain alkanes such as dodecane, hexasiloxane and silane were identified in the extract of fungus incubated with LDPE film through GC–MS analysis which might be due to the degradation of LDPE film by C. sphaerospermum. This was the first report on the LDPE degradation by C. sphaerospermum in very short duration which enables green scavenging of plastic wastes.

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Section: Resultssupporting

confidence: 93%

Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere

Sathiyabama,

Boomija,

Sathiyamoorthy

et al. 2024

Sci Rep

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“…The stretching vibration of free methyl and CC groups reflects the partial breaking of PE bonds. Researchers generally believe that fungal laccases play a certain role in the degradation of PE, but the underlying mechanism has not been studied and reported 34,35 . Our experiments showed that the particle size of PE affects the process of its degradation.…”

Section: Discussionmentioning

confidence: 62%

“…Researchers generally believe that fungal laccases play a certain role in the degradation of PE, but the underlying mechanism has not been studied and reported. 34,35 Our experiments showed that the particle size of PE affects the process of its degradation. In addition, laccase plays an important role in the early degradation of PE, which is mainly reflected in the oxidation of PE and the breaking of long chains.…”

Section: Discussionmentioning

confidence: 73%

Degradation of polyethylene particles by Trichoderma harzianum and recombinant laccase cloned from the strain

Ruan,

Hu,

Zhao

et al. 2023

J of Applied Polymer Sci

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Polyethylene (PE) is a convenient and inexpensive polymer that is widely used in daily life. However, PE products are recalcitrant to degradation in the environment, leading to increasing plastic pollution. This has inspired the search for effective microorganisms and enzymes that can biodegrade PE. In this study, a fungal strain that degrades PE was isolated from soil contaminated with plastic and identified as Trichoderma harzianum. The strain was cultured with PE as the sole carbon source for 30 days, and the weight loss of PE particles was 3.39 ± 0.3%. Scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectra showed holes and oxygenated functional groups on the surface of the degraded PE particles. Gas chromatography–mass spectrometry (GC–MS) detected products with different carbon atom numbers. In addition, a potential PE degrading enzyme was screened from T. harzianum and identified as laccase. After PE was incubated with the recombinant laccase for 96 h, SEM, FTIR, and GC–MS showed the degradation of PE particles. The results showed that T. harzianum had the ability to degrade PE, which probably was attributed to the laccase produced by this strain. This study contributes promising tools for the degradation of PE enzyme.

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“…PE is a high molecular weight polymer linked by a C-C single bond [240]. PE is one of the most inert plastics, and its resistant nature is due to its high molecular weight, complex three-dimensional structure and hydrophobic properties that make it difficult for microorganisms to access [239].…”

Section: Plasticizersmentioning

confidence: 99%

“…In the work of Zhang et al ( 2022), recombinant laccase from Acinetobacter baumannii Rd-H2 was obtained isolated from a grain and food storage pest [240]. The recombinant AbMCO enzyme led to the occurrence of basic structural changes on the surface of the PE film.…”

Section: Plasticizersmentioning

confidence: 99%

Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

Gałązka¹,

Jankiewicz²,

Szczepkowski³

2023

Preprint

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Industrialization, intensive farming, rapid population growth and urbanization are the source of a large number of pollutants entering the environment. The current concentration of xenobiotics released into the environment exceeds its natural ability to decompose them. Enzymatic degradation of pollutants seems to be an environmentally friendly process. Due to the wide spectrum of substrate specificity, from inorganic compounds to high molecular weight organic compounds such as PAH or dyes, as well as favorable biochemical properties, laccase has been used in the biological removal of xenobiotics from the environment. It is important to understand the degradation mechanisms of pollutants and to evaluate the final products in terms of their toxicity. The laccase oxidizes the substrates with the simultaneous reduction of molecular oxygen to water, which is the purest reaction co-substrate. That is why it is called a green biocatalyst. The trend is an increase in the production of enzymes related to the intensive development of industry, bioremediation or synthetic chemistry. This leads to the search for laccases with greater activity and stability under extreme conditions. The potential of laccases to degrade xenobiotics can be promoted by improving enzymatic catalytic characterization using protein engineering and other genetic engineering methods.

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Screening of Polyethylene-Degrading Bacteria from Rhyzopertha Dominica and Evaluation of Its Key Enzymes Degrading Polyethylene

Cited by 22 publications

References 34 publications

Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere

Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere

Degradation of polyethylene particles by Trichoderma harzianum and recombinant laccase cloned from the strain

Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

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