Screening of poultry-pig farms for methicillin-resistant Staphylococcus aureus: Sampling methodology and within herd prevalence in broiler flocks and pigs

Pletinckx, Larissa J; Verhegghe, Marijke; Dewulf, Jeroen; Crombé, Florence; Bleecker, Y. De; Rasschaert, Geertrui; Goddeeris, Bruno; Man, Ingrid De

doi:10.1016/j.meegid.2011.07.008

Cited by 38 publications

(30 citation statements)

References 20 publications

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“…This phenomenon was also observed by Pletinckx et al (10). They analyzed three different production cycles on three broiler farms within a time frame of 1 year.…”

Section: Discussionsupporting

confidence: 74%

“…They found 5.4% of these to be MRSA positive but did not report the sample matrix of origin of these MRSA strains (29). In a Belgian study, air and environmental samples (floor, wall, feed, or manure) from three different broiler farms tested negative when specifically examined for the occurrence of MRSA (10). However, one of the farms in that study was completely negative for MRSA, regardless of type of sample.…”

Section: Discussionmentioning

confidence: 93%

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Occurrence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus in Turkey and Broiler Barns and Contamination of Air and Soil Surfaces in Their Vicinity

Friese¹,

Schulz

Zimmermann³

et al. 2013

Appl Environ Microbiol

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The emission of microorganisms, especially resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA), from poultry farms is of public interest, and its occurrence and relevance are controversially discussed. So far, there are limited data on this issue. In this study, we investigated the occurrence of livestock-associated (LA)-MRSA inside and outside previously tested MRSA-positive poultry barns in Germany. In total, five turkey and two broiler fattening farms were investigated four and three times, respectively. In a longitudinal study during one fattening period, samples were collected from animals, the animals' environment inside the barn, including the air, and the barns' surroundings, such as ambient air and boot swabs of ground surfaces at different distances from the barn. Moreover, a cross-sectional study was carried out once inside the barns on five turkey and four broiler farms during the last third of the fatting period. In the cross-sectional study, LA-MRSA was detected in the air of most barns (7 of 9, 77.8%), as well as in many samples originating from animals, with detections levels of 50 to 54% in broiler and 62 to 77% in turkey farms. In the longitudinal study, LA-MRSA was found in the ambient air outside two turkey barns and on the ground surface on the downwind side of many (44.4%) turkey and broiler farms. The same spa types of isolates were observed inside and outside the barns. Transmission of MRSA within poultry farms, as well as emission via the airborne route, seems to be possible.

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“…This phenomenon was also observed by Pletinckx et al (10). They analyzed three different production cycles on three broiler farms within a time frame of 1 year.…”

Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 93%

Occurrence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus in Turkey and Broiler Barns and Contamination of Air and Soil Surfaces in Their Vicinity

Friese¹,

Schulz

Zimmermann³

et al. 2013

Appl Environ Microbiol

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“…The MRS isolation rate recorded in this study is higher than 34.6 and 41% MRS isolation rate reported by Mai-siayama et al (2014) and Waters et al (2011) from ruminants and processed chicken in Northeast Nigeria and America, respectively. Pletinckx et al (2010) reported 44.4 and 16.7% MRSA isolation rate from claoca and skin beneath the wings of broilers, respectively. The variation in the isolation rates in these studies may be related to differences in the rate of contamination of the handlers, farm environments and the animals.…”

Section: Discussionmentioning

confidence: 98%

Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, Nigeria

et al. 2015

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This study was conducted to isolate and detect methicillin-resistant staphylococci (MRS) in healthy broilers in Nsukka Southeast, Nigeria and determine the antibiogram of the isolates. Cloacal and skin swabs were collected from each of 101 randomly sampled broilers meant for slaughter. The samples were processed for isolation and identification of methicillin-resistant Staphylococcus species, following standard methods. Confirmation of methicillin-resistance by the isolates was done using penicillin binding protein 2a (PBP2a) kit. Phenotypic resistance of the isolates to antimicrobial agents was determined using disc diffusion method. Out of 202 samples processed, 200 (99.01%) yielded positive growth of staphylococci on oxacillin-supplemented oxacillin-resistance staphylococcal agar base (ORSAB). A total of 200 methicillin-resistant staphylococcal isolates were obtained. Of these, 91 (45.5%) were identified as methicillin-resistant coagulase-positive Staphylococcus (MRCoPS), while 109 (54.5%) were identified as methicillin-resistant coagulase-negative Staphylococcus species (MRCoNS). Out of the 91 MRCoPS, 53 (58.2%) were identified as methicillin-resistant Staphylococcus aureus (MRSA). Resistance of the isolates was 99.5% to erythromycin and chloramphenicol, 100% to oxacillin, 76.5% to gentamicin, 96.5% to clindamycin, 92.5% to ciprofloxacin, 99% to sulphamethoxazole/trimethoprim and tetracycline, and 98.5% to streptomycin and cefoxitin. All the isolates were multidrug resistant. This study has shown that healthy broilers reared and slaughtered in Nsukka Southeast, Nigeria harbour multidrugresistant MRS and thus serve as their reservoirs.

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“…Other studies demonstrated MRSA in raw chicken meat (Kitai et al, 2005;Lee, 2006;Dohoo et al, 2009) and broilers at slaughterhouses (Mulders et al, 2010). LA-MRSA isolates in poultry have previously been found in the Netherlands (Leenders et al, 2007;Geenen et al, 2012) and Belgium (Nemati et al, 2008;Persoons et al, 2009;Vanderhaeghen et al, 2010a;Pletincks et al, 2011). However, these studies were rather limited and different isolation methods were used.…”

Section: Introductionmentioning

confidence: 99%

Characterization of methicillin-resistantStaphylococcus aureusfrom healthy carrier chickens

et al. 2013

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Screening of poultry-pig farms for methicillin-resistant Staphylococcus aureus: Sampling methodology and within herd prevalence in broiler flocks and pigs

Cited by 38 publications

References 20 publications

Occurrence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus in Turkey and Broiler Barns and Contamination of Air and Soil Surfaces in Their Vicinity

Occurrence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus in Turkey and Broiler Barns and Contamination of Air and Soil Surfaces in Their Vicinity

Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, Nigeria

Characterization of methicillin-resistantStaphylococcus aureusfrom healthy carrier chickens

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