Screening of Probiotic Lactic Acid Bacteria Isolated from Fermented Pak-Sian for Use as a Starter Culture

Pumriw, Supaporn; Luang-In, Vijitra; Samappito, Wannee

doi:10.1007/s00284-021-02521-w

Cited by 12 publications

(8 citation statements)

References 47 publications

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“…According to the Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO) regulations (2002), probiotics must have a high tolerance to the gastrointestinal (GIT) conditions as well as high adherence in order to serve as a probiotic and maintain the balance of the intestinal microbiota ( Hardy et al, 2013 ; Monteagudo-Mera et al, 2019 ). Studies have shown that gastrointestinal conditions (pH, bile, digestive enzymes) and the acid and bile salt tolerance of bacteria have important impacts on the adhesion ability of probiotics ( Damaceno et al, 2021 ; Pumriw et al, 2021 ; Tarrah et al, 2021 ). To ensure that L. plantarum is able to survive after passage through the gastrointestinal tract, evaluating the effect of acid-base stress on this organism has important significance for studying its adhesion ability.…”

Section: Introductionmentioning

confidence: 99%

Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Zhao

Yang

et al. 2022

Front. Microbiol.

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The beneficial effects of the probiotic strain Lactiplantibacillus plantarum (formerly Lactobacillus plantarum) are based on its adherence and colonization ability in the gut. However, little is known about the migration and long-term gut colonization of the strain. This study evaluated the gut colonization modes of Lactiplantibacillus plantarum RS-09 to identify the strain with long-term gut colonization potential. We established CFDA/SE-labeled RS-09 to study the temporal and spatial distribution of RS-09 in the intestine as well as to analyze its persistence in different parts of the intestine by flow cytometry. This study has shown that the RS-09 strain maintains strong adhesion abilities under acid (pH 2.5) and base (pH 8.5) conditions. In addition, CFDA/SE can be used as an indicator for the labeling of L. plantarum RS-09 in the intestinal tract in vivo. We established a growth kinetics model of RS-09 to elucidate its persistence in the intestine. In vivo persistence experiments showed that the persistence rate of RS-09 was the highest in the cecum (69.5%) and the lowest in the duodenum (12.8%) at 96 h. After 20 days, RS-09 was predominantly localized in the cecum and colon steadily. These studies provide new insights into the long-term persistence of L. plantarum in the gastrointestinal tract. The CFDA/SE label system may be used to study the in vivo colonization dynamics of other probiotic strains.

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Section: Introductionmentioning

confidence: 99%

Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Zhao

Yang

et al. 2022

Front. Microbiol.

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“…The previous study reported the concentration of GABA which was produced by LAB with various amount. 24,15,3 The concentration of GABA related with several variable parameters such as starter inoculum, MSG concentration and glucose supplementation. 25 According the amount of GABA from this study, this suggested that the optimization for high yield production was needed to be investigated in the future.…”

Section: Discussionmentioning

confidence: 99%

“…The isolation of L. pentosus from Pak Sian Dong has been reported previously. 3 In general, L. pentosus is a gram positive, rod shape which can be detected in various environments including plants, animals and fermented foods. Several studies reveald that L. pentosus can be used as probiotics in various aspects such as antimicrobial activity, 27 cholesterol reduction 28 and immunomodulation.…”

Section: Discussionmentioning

confidence: 99%

“…For the community of microorganism in fermented spider plant, there are a group of lactic acid bacteria (LAB), such as Pediococcus pentosaceus, Lactiplantibacillus plantarum and Levilactobacillus brevis, and these bacteria were characterized for probiotic activities. 3 Probiotics are commonly defined as the live organisms that can provide beneficial effects to the hosts. 4 Various aspects of probiotics can be used such as prevention of infection, promotion of the health and treatment of the diseases.…”

Section: Introductionmentioning

confidence: 99%

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Gamma-aminobutyric Acid (GABA) Producing Probiotic Lactiplantibacillus Pentosus Isolated from Fermented Spider Plant (Pak Sian Dong) in Thailand

Amatachaya¹,

Siramolpiwat²,

Kraisorn³

et al. 2023

J. Pure Appl. Microbiol.

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Psychobiotics are defined as the organisms that can provide the mental health benefit. The possible mechanism of psychobiotics is manipulation of neurotransmitter production and neurotransmitter production by the microbes. The lactobacillus group has been reported for the potential of neurotransmitter production, especially g-aminobutyric acid (GABA) which is an important inhibitory neurotransmitter. Therefore, GABA can be used for relaxation and applied in various psychiatric disorders. The aim of this study was determination of lactic acid bacterial isolates from Pak Sian Dong in Thailand for GABA producing ability. The results found that there were 3 isolates, SF66, SF80 and SF82, which revealed the ability to produce glutamic acid decarboxylase (GAD) enzyme. The GABA were detected by thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) in the bacterial culture containing 3% monosodium glutamate. The survival in gastrointestinal synthetic condition found that only SF66 isolate showed the authentic percentage of survival then this isolate was selected. From the identification, the isolate was identified as Lactiplantibacillus pentosus and was designated as L. pentosus SF66 which exhibited with the potential for further investigation and development to be psychobiotics.

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“…A lysogenic broth (LB) medium was used to grow E. coli F5 (BNCC125787) and S. Dublin (BNCC186358). The anti-pathogenic activity of the LAB isolates against E. coli F5 and S. Dublin was evaluated using the Oxford cup method [ 25 ]. Briefly, petri dishes (10 cm), containing 5 mL of LB agar and sterilized Oxford cups on top, were overlaid with 10 mL of soft LB agar seeded with 10 7 CFU/mL pathogenic bacteria at 45 °C.…”

Section: Methodsmentioning

confidence: 99%

Lactiplantibacillus plantarum A1, C1 and C10 Are Potential Probiotics Isolated from Pineapple Residual Silage

et al. 2022

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The production and consumption of pineapple creates large quantities of residues. Ensiling these residues might help to minimize the waste burden and meet the intensive feed demand for ruminants. Proper lactic acid bacteria (LAB) are not only responsible for pineapple residual silage fermentation, but might also deliver probiotics. The aim of this study was to isolate LAB strains with probiotic functions, and to enhance intestinal antioxidant capacity from naturally fermented pineapple residues. A total of 47 LAB isolates with gram-positive, catalase-negative, nonhemolytic properties were used for probiotic screening. Lactiplantibacillus plantarum (L. plantarum) A1, C1 and C10 were susceptible to rifampicin, gentamicin and erythromycin, did not contain virulence factor-coding genes and showed good tolerance to acid (pH 3.0), 0.5% bile salt and simulated gastric and intestinal fluid. Their hydrophobicity indices were 71.92%, 45.50% and 66.90%, respectively. All of them were able to adhere to bovine jejunum epithelial cells (BJECs) and to antagonize Escherichia coli F5 and Salmonella Dublin. These three LAB strains tolerated hydrogen peroxide and significantly decreased (p < 0.05) reactive oxygen species levels in BJECs. In addition, L. plantarum C1 and C10 significantly increased (p < 0.05) the total antioxidant capacity in BJECs in the presence of 200 μmol/L hydrogen peroxide condition. L. plantarum A1, C1 and C10 are potential probiotics isolated from pineapple residual silage. This study aims to promote pineapple residue’s utilization in the feed industry.

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Screening of Probiotic Lactic Acid Bacteria Isolated from Fermented Pak-Sian for Use as a Starter Culture

Cited by 12 publications

References 47 publications

Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Gamma-aminobutyric Acid (GABA) Producing Probiotic Lactiplantibacillus Pentosus Isolated from Fermented Spider Plant (Pak Sian Dong) in Thailand

Lactiplantibacillus plantarum A1, C1 and C10 Are Potential Probiotics Isolated from Pineapple Residual Silage

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