Screening of promising chemotherapeutic candidates from plants extracts

Kinjo, Junei; Nakano, Daisuke; Fujioka, Toshihiro; Okabe, Hikaru

doi:10.1007/s11418-016-0992-2

Cited by 29 publications

(24 citation statements)

References 37 publications

(40 reference statements)

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“…Recently, it was shown that calotropin (11) inhibits Wnt signaling by increasing casein kinase 1α in colon cancer cells 19) and 11 induces cell cycle arrest and apoptosis in cisplatinresistant lung cancer cells. 20) In conclusion, as part of our investigations of Asclepiadaceae plants, extracts of A. curassavica showed potent inhibitory effects against MT-1 and MT-2 cell lines. We isolated 17 cardenolides from the extract and examined their structureactivity relationships.…”

Section: Resultsmentioning

confidence: 76%

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Screening of Promising Chemotherapeutic Candidates from Plants against Human Adult T-Cell Leukemia/Lymphoma (VI): Cardenolides from <i>Asclepias curassavica</i>

Nakano

Ishitsuka

Takashima

et al. 2020

Biological & Pharmaceutical Bulletin

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In the course of our screening program for novel chemotherapeutic candidates from plants against adult T-cell leukemia/lymphoma, the extracts of Asclepias curassavica L. showed potent activity against MT-1 and MT-2 cells. Therefore, we attempted to isolate their active components. We identified a new cardenolide, 19-dihydrocalactinic acid methyl ester (1), along with 16 known cardenolides (2-17). Their structures were determined on the basis of spectroscopic data. Almost all of the isolated cardenolides inhibited the growth of both tumor cell lines. All the doubly linked cardenolides (11-17) except for 14 showed more potent activity than the other cardenolides. A comparison of the activities of 11, 14 and 16 revealed that the presence of hydroxy or acetoxy functional groups at C-16 led to a decrease in the activity. The 50% effective concentration (EC 50) value of calotropin (11) against MT-2 cells was comparable to the potency of the clinical antineoplastic drug doxorubicin. The cytotoxic effect of 11 toward normal mononuclear cells obtained from the peripheral blood (PB-MNCs) was observed at a concentration 6 to 12 times higher than that used to induce growth inhibition against MT-1 and MT-2 cells. The proportions of annexin V-positive cells after 72 h of treatment with 11 were increased, indicating that it significantly induced apoptosis in MT-1 and MT-2 cells in a concentration-dependent manner. Cell cycle experiments demonstrated that 11 arrested MT-1 and MT-2 cells at the G2/M phase. Therefore, compound 11 may be a promising candidate for the treatment of adult T-cell leukemia/lymphoma.

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Section: Resultsmentioning

confidence: 76%

“…Therefore, cardenolides are promising candidates for the treatment of ATL, similar to withanolides. 21)…”

Section: Resultsmentioning

confidence: 99%

Screening of Promising Chemotherapeutic Candidates from Plants against Human Adult T-Cell Leukemia/Lymphoma (VI): Cardenolides from <i>Asclepias curassavica</i>

Nakano

Ishitsuka

Takashima

et al. 2020

Biological & Pharmaceutical Bulletin

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“…The higher lipophilicity of polyacetylene compounds facilitates plasma membrane penetration but often lowers their bioavailability in vivo (36). A comparison between different plant-derived polyacetylenes by Kinjo et al (46) suggested an inverse relationship of the presence of bulky side chains (hydroxy, methoxy, amino or other groups) with activity and effectiveness. The authors also found that C. intybus polyacetylenes were more effective against MK-1 cells than HeLa and B16F10 cells, and their antiproliferative effects were more profound than other chemoprotective characteristics (46).…”

Section: Antitumor Activity Of the Phytochemicalsmentioning

confidence: 99%

“…A comparison between different plant-derived polyacetylenes by Kinjo et al (46) suggested an inverse relationship of the presence of bulky side chains (hydroxy, methoxy, amino or other groups) with activity and effectiveness. The authors also found that C. intybus polyacetylenes were more effective against MK-1 cells than HeLa and B16F10 cells, and their antiproliferative effects were more profound than other chemoprotective characteristics (46). In estrogen-responsive breast cancer cells (ZR-75-1), both putrescine and spermidine were internalized via the same or similar transporter and both V max was rapidly upregulated by estrogens and insulin (47).…”

Section: Antitumor Activity Of the Phytochemicalsmentioning

confidence: 99%

Cytotoxicity of Cichorium intybus L. metabolites (Review)

et al. 2019

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Cichorium intybus L. (Chicory) is a widely distributed, edible, perennial, herbaceous member of the Asteraceae family. Besides its use in modern Chinese herbal medicine, its ethnomedicinal use is evident in the text from ancient Greece, Egypt and China. It is also used as a food and coffee substitute, which is mainly responsible for its extensive domestication. In recent decades, cytotoxic studies of C. intybus extracts have shown its antitumor potential. These studies also identified metabolite constituents including guaianolides, 6-methoxyflavone, eudesmanolides, germacranolides, polyacetylene, sterol, anthocyanin, delphinidin, 3,4-dihydroxyphenethyl and other novel compounds. Many of these phytometabolites have shown positive cytotoxic activities in vitro, and antitumor action in vivo and in clinical trials, demonstrating the potential of C. intybus metabolites as antitumor drugs. Structural activity relationship studies have further confirmed these bioactivities. In this review, we focused on the phytochemicals of C. intybus with reported cytotoxicity and potential antitumor properties. We also discuss their specificity towards tumor cells, structural activity relationship, the involved signaling pathways and molecular mechanism, with the expectation of the future development of efficient and targeted antitumor therapeutic strategies.

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“…In addition, many cancers still exhibit modest clinical responses to the usual protocols or develop chemotherapy resistance [4]. Thus, in order to achieve more effective and safer molecules, pharmacological studies with substances isolated from plants have been intensified, as well as, synthetic derivatives from these natural compounds [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

In vitro Antiproliferative Potential of Celtis iguanaea against Ovarian (OVCAR-3) and Colon (HT-29) Tumor Cell

Zanchet

Miorando

Gomes

et al. 2019

EJMP

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Natural products have been reported as a main source of anticancer molecules. The species Celtis iguanaea (Jacq.) Sarg., (Cannabaceae) is widely distributed in Brazil where it is known as “esporão-de-galo or taleira”. The leaves are popularly used as anti-inflammatory, in the treatment of body pain and urinary infections. However, the antiproliferative potential against human cancer cells remain to be elucidated. In this study, extracts and different fractions from the leaves of C. iguanaea were tested in vitro, against a panel tumor cell lines. The hydroalcoholic extract was inactive, while dichloromethane extract showed promisor antiproliferative effects. In turn, the dichloromethane fraction showed potent cytostatic activity against ovarian cell line (OVCAR-3, GI50 = 4.65 mg/ml). However, the stronger antiproliferative effects with high selectivity were observed for the hexane fraction that exhibited activity against ovarian (GI50 = 3.99 mg/ml) and colon (HT-29, GI50 = 3.16 mg/ml). The antiproliferative effects observed are probably related to the presence of 3,7,11,15-tetramethyl-2-hexadecen-1-ol and gamma-sitosterol present in the hexane fraction and detected by GC/MS. This is the first report of antiproliferative activity of C. iguanaea and the results suggested that the molecules of fraction hexane are promising chemotherapeutic compounds, especially against tumor cell of ovarian and colon.

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Screening of promising chemotherapeutic candidates from plants extracts

Cited by 29 publications

References 37 publications

Screening of Promising Chemotherapeutic Candidates from Plants against Human Adult T-Cell Leukemia/Lymphoma (VI): Cardenolides from <i>Asclepias curassavica</i>

Screening of Promising Chemotherapeutic Candidates from Plants against Human Adult T-Cell Leukemia/Lymphoma (VI): Cardenolides from <i>Asclepias curassavica</i>

Cytotoxicity of Cichorium intybus L. metabolites (Review)

In vitro Antiproliferative Potential of Celtis iguanaea against Ovarian (OVCAR-3) and Colon (HT-29) Tumor Cell

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