Screening of protease-producing Serratia marcescens FS-3 and its application to deproteinization of crab shell waste for chitin extraction

Jo, Gyung-Hyun; Jung, Woo‐Jin; Kuk, Ju-Hee; Oh, Kyung-Taek; Kim, Youn Joong; Park, R.D.

doi:10.1016/j.carbpol.2008.04.019

Cited by 88 publications

(40 citation statements)

References 9 publications

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“…However, the use of these chemicals may cause a partial deacetylation of the chitin and hydrolysis of the polymer resulting in final inconsistent physiological properties [7]. To overcome the hazards from chemical treatments, alternative methods on use of microorganisms [8][9][10] or proteolytic enzymes [11] for the deproteinization of crustacean wastes have been reported.…”

Section: Introductionmentioning

confidence: 99%

An Oxidant- and Solvent-Stable Protease Produced by Bacillus cereus SV1: Application in the Deproteinization of Shrimp Wastes and as a Laundry Detergent Additive

Manni

Jellouli

Ghorbel-Bellaaj

et al. 2009

Appl Biochem Biotechnol

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The current increase in amount of shrimp wastes produced by the shrimp industry has led to the need in finding new methods for shrimp wastes disposal. In this study, an extracellular organic solvent- and oxidant-stable metalloprotease was produced by Bacillus cereus SV1. Maximum protease activity (5,900 U/mL) was obtained when the strain was grown in medium containing 40 g/L shrimp wastes powder as a sole carbon source. The optimum pH, optimum temperature, pH stability, and thermal stability of the crude enzyme preparation were pH 8.0, 60 degrees C, pH 6-9.5, and <55 degrees C, respectively. The crude protease was extremely stable toward several organic solvents. No loss of activity was observed even after 60 days of incubation at 30 degrees C in the presence of 50% (v/v) dimethyl sulfoxide and ethyl ether; the enzyme retained more than 70% of its original activity in the presence of ethanol and N,N-dimethylformamide. The protease showed high stability toward anionic (SDS) and non-ionic (Tween 80, Tween 20, and Triton X-100) surfactants. Interestingly, the activity of the enzyme was significantly enhanced by oxidizing agents. In addition, the enzyme showed excellent compatibility with some commercial liquid detergents. The protease of B. cereus SV1, produced under the optimal culture conditions, was tested for shrimp waste deproteinization in the preparation of chitin. The protein removal with a ratio E/S of 20 was about 88%. The novelties of the SV1 protease include its high stability to organic solvents and surfactants. These unique properties make it an ideal choice for application in detergent formulations and enzymatic peptide synthesis. In addition, the enzyme may find potential applications in the deproteinization of shrimp wastes to produce chitin.

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Section: Introductionmentioning

confidence: 99%

An Oxidant- and Solvent-Stable Protease Produced by Bacillus cereus SV1: Application in the Deproteinization of Shrimp Wastes and as a Laundry Detergent Additive

Manni

Jellouli

Ghorbel-Bellaaj

et al. 2009

Appl Biochem Biotechnol

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“…However, the use of these chemicals may destroy the chitin. To overcome the hazards from chemical treatments, alternative methods on use of microorganisms (Ghorbel-Bellaaj et al 2011a;Jo et al 2008) or proteolytic enzymes (Ghorbel-Bellaaj et al 2011b;Manni et al 2010) for the deproteinization of crustacean wastes have been reported.…”

Section: Introductionmentioning

confidence: 99%

Zebra blenny (Salaria basilisca) viscera as a source of solvent-stable proteases: characteristics, potential application in the deproteinization of shrimp wastes and evaluation in liquid laundry commercial detergents

et al. 2012

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The present study describes the characterization of crude protease extract from zebra blenny (Salaria basilisca) and its evaluation in liquid detergent and shrimp waste deproteinization. At least five caseinolytic proteases clear bands were observed in zymogram. The crude alkaline protease showed optimum activity at pH 8.0 and 60°C, and it was highly stable over a wide range of pH from 6.0 to 11.0. Proteolytic enzymes showed extreme stability towards non-ionic surfactants (5 % Tween 80 and 5 % Triton X-100) and oxidizing agents (1 % sodium perborate), and relative stability towards anionic surfactant (1 % Sodium dodecyl sulfate (SDS)). They also showed high stability and compatibility with various laundry liquid detergents from Tunisian market. Furthermore, the crude enzyme was stable towards several organic solvents and retained more than 50 % of its original activity after 30 days of incubation at 30°C in the presence of 50 % (v/v) dimethylsulfoxide (DMSO). Further, proteases from zebra blenny viscera were found to be effective in the deproteinization of shrimp wastes. The protein removal after 3 h at 40°C with an enzyme/substrate ratio (E/S) of 5 U/mg protein was about 77 %.

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“…For the deproteination of crustacean shells, proteases from bacteria are used. The treatment consists of a fermentation of the crustacean by diferent species of bacteria such as Pseudomonas aeruginosa K-187, Serratia marcescens FS-3, and Bacillus subtilis [24]. Commercial proteases have previously been used to produce chitosan [25].…”

Section: Enzymatic Methodsmentioning

confidence: 99%

Chitosan, Chitosan Derivatives and their Biomedical Applications

Muñoz¹,

Corrales²

2017

Biological Activities and Application of Marine Polysaccharides

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Chitosan is one of the most studied polysaccharides nowadays. Because of its biocompatibility, biodegradability and abundance in nature, it has had a wide number of applications. In this chapter, an overview of chitosan including its physicochemical properties and characterization methods is presented. Subsequently, the main chitosan chemical modiications via the hydroxyl and amino groups are discussed. These chemical modiications improve chitosan physical properties and expand its range of applications especially in the biomedical ield which will also be studied.

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Screening of protease-producing Serratia marcescens FS-3 and its application to deproteinization of crab shell waste for chitin extraction

Cited by 88 publications

References 9 publications

An Oxidant- and Solvent-Stable Protease Produced by Bacillus cereus SV1: Application in the Deproteinization of Shrimp Wastes and as a Laundry Detergent Additive

An Oxidant- and Solvent-Stable Protease Produced by Bacillus cereus SV1: Application in the Deproteinization of Shrimp Wastes and as a Laundry Detergent Additive

Zebra blenny (Salaria basilisca) viscera as a source of solvent-stable proteases: characteristics, potential application in the deproteinization of shrimp wastes and evaluation in liquid laundry commercial detergents

Chitosan, Chitosan Derivatives and their Biomedical Applications

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