Screening of resistant Indonesian black rice cultivars against bacterial leaf blight

Sutrisno, Sutrisno; Susanto, Febri Adi; Wijayanti, Putri; Retnoningrum, Mita Dewi; Nuringtyas, Tri Rini; Joko, Tri; Purwestri, Yekti Asih

doi:10.1007/s10681-018-2279-z

Cited by 21 publications

(23 citation statements)

References 29 publications

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“…A new pathogen race destroys the resistance of a plant variety. Plant varieties with vertical resistance (resistance is determined by a single gene or a few genes and only effective against some strains of pathogen) need replacement of resistance every few years (3, 5, or 10 years), and this is depending on the pathogen genetic plasticity, a particular gene, or a genetic combination, as well as environmental conditions on the development of the disease (Laeshita and Arwiyanto, 2017;Sutrisno et al 2018). Deberdt et al (2014) also reported that tomato cultivar H-7996 is known to be resistant to R. solanacearum but changing to be susceptible after being inoculated with R. solanacearum strains IIB, which is an emerging ecotype.…”

Section: Analysis Of Ralstonia Solanacearum and Measurement Of Bacterial Wilt Disease Intensitymentioning

confidence: 99%

Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum

et al. 2020

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Abstract. Navitasari L, Joko T, Murti RH, Arwiyanto T. 2020. Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum. Biodiversitas 21: 4888-4895. Bacterial wilt disease caused by Ralstonia solanacearum is a devastating soil-borne vascular disease of tomato leading to a 100% yield loss. One of the alternatives to suppress the infestation of R. solanacearum infestation is the application of grafting techniques, which has been studied and successfully practiced by tomato growers. However, the infestation mode of R. solanacearum and the rhizobacterial community structure in grafted tomato plants are poorly reported. In this study, the rhizobacterial community structure in grafted tomato plants infected by R. solanacearum was investigated. The experiment was conducted on tomato germplasms with the implementation of tube grafting using resistant rootstocks (Amelia from Indonesia, H.7996 from Asian Vegetable Research Development Center/AVRDC) and susceptible scion (Servo from Indonesia). The rhizobacterial community structure was analyzed by metagenomic study under 16S rRNA genes sequencing with a distinct region (16SV3-V4) that was amplified using a specific primer (16SV4: 515F-806R) 5’-GTGCCAGCMGCCGCGGTAA and 5’GGACTACHVHHHTWTCTAAT. The results indicated that the grafted tomato plants and resistant rootstocks that were infected by R. solanacearum showed significantly lower intensity of bacterial wilt disease compared to the susceptible scion. The rhizobacterial community structure in the grafted tomato plants infected by R. solanacearum was indicated by predominant phyla of Proteobacteria, Firmicutes, and Actinobacteria with dominant genera of Pseudomonas and Bacillus. Besides, significant difference was also indicated by species of Geitlerinema sp. in the grafted tomatoes infected by R. solanacearum.

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Section: Analysis Of Ralstonia Solanacearum and Measurement Of Bacterial Wilt Disease Intensitymentioning

confidence: 99%

Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum

et al. 2020

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“…The development of BLB during observation in this study could be analyzed using the Area Under Disease Progress Curve (AUDPC) obtained from the disease intensity. AUDPC values were calculated according to Sutrisno et al (2018) as follows:…”

Section: The Resistance Of Rice Against Bacterial Leaf Blightmentioning

confidence: 99%

The Resistance of Local Pigmented Rice Varieties against Bacterial Leaf Blight

Joko

Kristamtini²,

Sumarno

et al. 2019

JPTI

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Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important disease in rice plants worldwide, including in Indonesia. The use of resistant variety against BLB is the best effort in disease control because it is considered as the most effective and environmentally friendly. This study aimed to evaluate the resistance of local varieties, red rice (cv. Sembada Merah) and black rice (cv. Sembada Hitam), against Xoo compared to white rice variety (Ciherang), commonly cultivated by the farmers. The study was conducted in a greenhouse and arranged in Factorial of Completely Randomized Design (CRD) with 5 replications and 2 treatment factors: variety (Sembada Hitam, Sembada Merah, and Ciherang) and inoculation (with and without inoculated by Xoo bacterial suspension). Xoo inoculation was performed by clipping the leaves that close to the reproductive phase when the heading begins to be produced. The disease intensity and Area Under Disease Progress Curve (AUDPC) over two weeks were used to determine the level of resistance of the tested plant varieties. The results showed that black rice and red rice had better levels of resistance against BLB compared to white rice. At two weeks after inoculation, Sembada Hitam had the lowest AUDPC value followed by Sembada Merah, while the Ciherang had the highest AUDPC value and was categorized as a susceptible variety to Xoo.

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“…The sample was prepared by mixing 5 µL of DNA with 2 µL of 1% gel red (Biotium). After electrophoresis, the gel was visualized under UV (300 nm) illumination (Mahfut et al 2016;Sutrisno et al 2018).…”

Section: Polymerase Chain Reaction and Primer Optimizationmentioning

confidence: 99%

A novel subspecies-specific primer targeting the gyrase B gene for the detection of Pectobacterium carotovorum subsp. brasiliense

2019

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Abstract. Joko T, Soffan A, Muhammad Saifur Rohman MS. 2019. A novel subspecies-specific primer targeting the gyrase B gene for the detection of Pectobacterium carotovorum subsp. brasiliense. Biodiversitas 20: 3042-3048. Pectobacterium carotovorum subsp. brasiliense is one of the major causative bacterial pathogens of the soft rot disease in various crops. It has a high virulence and a wide range of hosts in the tropics and the subtropics. Most often, conventional methods are not able to accurately distinguish P. carotovorum subsp. brasiliense from other subspecies. Thus, this study aimed to design a specific gyrase B gene (gyrB) -based primers for the detection and identification of soft rot pathogen. The specific primers design was based on the alignment using gyrB gene sequence data from P. carotovorum subsp. brasiliense and other data from the GenBank. The primers comprised of F-gyr-Pcb (5’-CAC AGG CAC CGC TGG CTG TT-3’) and R-gyr-Pcb (5’-CGT CGT TCC ACT GCA ATG CCA-3’) with an amplicon of 336 base pairs. The specificity of the primers pair was verified both in silico and in polymerase chain reaction (PCR) assays, where the primers could only detect P. carotovorum subsp. brasiliense. Primers’ sensitivity was determined by qualitative PCR with a detection limit of less than 0.5 ng/µL of genomic DNA. Hence, the proposed detection tool can be beneficial to advance further studies on the ecology and epidemiology of soft rot diseases.

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Screening of resistant Indonesian black rice cultivars against bacterial leaf blight

Cited by 21 publications

References 29 publications

Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum

Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum

The Resistance of Local Pigmented Rice Varieties against Bacterial Leaf Blight

A novel subspecies-specific primer targeting the gyrase B gene for the detection of Pectobacterium carotovorum subsp. brasiliense

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