Screening of<scp>C</scp>hinese cabbage mutants produced by<sup>60</sup><scp>C</scp>o γ‐ray mutagenesis of isolated microspore cultures

Huang, Shengnan; Liu, Zhiyong; Li, Danyang; Yao, Runpeng; Meng, Qin; Feng, Hui

doi:10.1111/pbr.12166

Cited by 30 publications

(19 citation statements)

References 30 publications

(21 reference statements)

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“…In this study, we characterized the drm exhibiting a slow-growing, chlorophyll-deficient phenotype, which was screened by Huang et al ( 2014 ) and derived from the Chinese cabbage DH line “FT” by a combination of isolated microspore culture and radiation treatment ( 60 Co γ-rays). Compared with the wild-type “FT” line, the chlorophyll contents were significantly reduced in drm , and the chloroplast structure was abnormal.…”

Section: Discussionmentioning

confidence: 99%

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Physiological Characterization and Comparative Transcriptome Analysis of a Slow-Growing Reduced-Thylakoid Mutant of Chinese Cabbage (Brassica campestris ssp. pekinensis)

Huang

Liu

et al. 2016

Front. Plant Sci.

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Mutants are ideal for studying physiological processes. The leaves of Chinese cabbage are a major place for photosynthesis, and the mutation of these leaves may directly affect the rate of plant growth and development, thus influencing the formation of its leafy head. We characterized a slow-growing mutant, which was designated as drm. The drm exhibited slow growth and development at the seedling and heading stages, leading to the production of a tiny, leafy head, and chlorophyll-deficient leaves, especially in seedlings. Genetic analysis indicated that the phenotype of drm was controlled by a single recessive nuclear gene. Compared with the wild-type “FT” line, the drm's chlorophyll content was significantly reduced and its chloroplast structure was abnormal. Moreover, its photosynthetic efficiency and chlorophyll fluorescence parameters were significantly decreased. The changes in leaf color, combined with these altered physiological characters, may influence the growth and development of plant, ultimately resulting in the slow-growing phenotype. To further understand the molecular regulation mechanisms of phenotypic differences between “FT” and drm, comparative transcriptome analyses were performed using RNA-Seq; a total of 338 differentially expressed genes (DEGs) were detected between “FT” and drm. According to GO and KEGG pathway analysis, a number of DEGs involved in chlorophyll degradation and photosynthesis were identified, such as chlorophyllase and ribulose-1,5-bisphosphate carboxylase/oxygenase. In addition, the expression patterns of 12 DEGs, including three chlorophyll degradation- and photosynthesis-related genes and nine randomly-selected genes, were confirmed by qRT-PCR. Numerous single nucleotide polymorphisms were also identified, providing a valuable resource for research and molecular marker-assistant breeding in Chinese cabbage. These results contribute to our understanding of the molecular regulation mechanisms underlying growth and development and lay the foundation for future genetic and functional genomics in Chinese cabbage.

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Section: Discussionmentioning

confidence: 99%

“…The drm was screened by Huang et al ( 2014 ) and derived from Chinese cabbage DH line “FT” by a combination of isolated microspore culture and radiation treatment ( 60 Co γ-rays). This mutant was diploid as determined by flow cytometry and exhibited stable inheritance after multiple generations.…”

Section: Methodsmentioning

confidence: 99%

Physiological Characterization and Comparative Transcriptome Analysis of a Slow-Growing Reduced-Thylakoid Mutant of Chinese Cabbage (Brassica campestris ssp. pekinensis)

Huang

Liu

et al. 2016

Front. Plant Sci.

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“…An etiolated mutant ( pem ) was obtained using the combination of 60 Co‐γ radiation and isolated microspore culture in Chinese cabbage (Huang et al ). The phenotype of the pem mutant was etiolated throughout the entire growth cycle of the plant.…”

Section: Introductionmentioning

confidence: 99%

Mutation in EMB1923 gene promoter is associated with chlorophyll deficiency in Chinese cabbage (Brassica campestris ssp. pekinensis)

Huang

et al. 2019

Physiologia Plantarum

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Leaf color mutants are widespread in higher plants and can be used as markers in crop breeding or as important material in understanding the regulatory mechanisms of chlorophyll biosynthesis and chloroplast development. A stably inherited plant etiolated mutation (pem) was obtained from its wild‐type ‘FT’ (a doubled haploid line of the Chinese cabbage variety ‘Fukuda 50’) by combining 60Co‐γ radiation and isolated microspore culture in Chinese cabbage. Compared to the wild‐type ‘FT’, the chlorophyll content in the pem mutant was decreased, the photosynthetic capacity was reduced and the chloroplast development was retarded. These physiological changes may lead to a reduction in growth and yield in the pem mutant line. Genetic analysis showed that the mutant phenotype was controlled by the single recessive nuclear pem gene. The pem gene was mapped to a 25.88 kb region on the A03 chromosome. Cloning and sequencing results showed that there was only one DNA sequence variation in this region, which was a 30 bp deletion on the promoter of Bra024218. Its homologous gene encodes EMBRYO DEFECTIVE 1923 (EMB1923) in Arabidopsis thaliana. We therefore predicted that Bra024218 was the mutated gene associated with etiolated leaves in Chinese cabbage. The pem mutant is a useful line for researching chloroplast development and the mechanism of leaf color mutation in Chinese cabbage.

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“…This strain was characterized by yellow leaves and elongated hypocotyls [44]. The parent used for the segregating population development with pylm in this study was 'FT', a DH line derived from Chinese cabbage variety 'Fukuda 50' screened by Shenyang greenstar Chinese cabbage research institute (Shenyang, China), which exhibits folded green leaves [68]. The pylm was reciprocally crossed with 'FT' to produce the F 1 , F 2 , and BC 1 generations.…”

Section: Plant Materials and Mapping Population Developmentmentioning

confidence: 99%

Identification of two recessive etiolation genes (py1, py2) in pakchoi (Brassica rapa L. ssp. chinensis)

Zhang

et al. 2020

BMC Plant Biol

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Background: Leaf color is a major agronomic trait, which has a strong influence on crop yields. Isolating leaf color mutants can represent valuable materials for research in chlorophyll (Chl) biosynthesis and metabolism regulation. Results: In this study, we identified a stably inherited yellow leaf mutant derived from 'Huaguan' pakchoi variety via isolated microspore culture and designated as pylm. This mutant displayed yellow leaves after germination. Its etiolated phenotype was nonlethal and stable during the whole growth period. Its growth was weak and its hypocotyls were markedly elongated. Genetic analysis revealed that two recessive nuclear genes, named py1 and py2, are responsible for the etiolation phenotype. Bulked segregant RNA sequencing (BSR-Seq) showed that py1 and py2 were mapped on chromosomes A09 and A07, respectively. The genes were single Mendelian factors in F 3:4 populations based on a 3:1 phenotypic segregation ratio. The py1 was localized to a 258.3-kb interval on a 34-gene genome. The differentially expressed gene BraA09004189 was detected in the py1 mapping region and regulated heme catabolism. One single-nucleotide polymorphism (SNP) of BraA09004189 occurred in pylm. A candidate genespecific SNP marker in 1520 F 3:4 yellow-colored individuals co-segregated with py1. For py2, 1860 recessive homozygous F 3:4 individuals were investigated and localized py2 to a 4.4-kb interval. Of the five genes in this region, BraA07001774 was predicted as a candidate for py2. It encoded an embryo defective 1187 and a phosphotransferase related to chlorophyll deficiency and hypocotyl elongation. One SNP of BraA07001774 occurred in pylm. It caused a single amino acid mutation from Asp to Asn. According to quantitative real-time polymerase chain reaction (qRT-PCR), BraA07001774 was downregulated in pylm. Conclusions: Our study identified a Chl deficiency mutant pylm in pakchoi. Two recessive nuclear genes named py1 and py2 had a significant effect on etiolation. Candidate genes regulating etiolation were identified as BraA09004189 and BraA07001774, respectively. These findings will elucidate chlorophyll metabolism and the molecular mechanisms of the gene interactions controlling pakchoi etiolation.

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Screening ofChinese cabbage mutants produced by⁶⁰Co γ‐ray mutagenesis of isolated microspore cultures

Cited by 30 publications

References 30 publications

Physiological Characterization and Comparative Transcriptome Analysis of a Slow-Growing Reduced-Thylakoid Mutant of Chinese Cabbage (Brassica campestris ssp. pekinensis)

Physiological Characterization and Comparative Transcriptome Analysis of a Slow-Growing Reduced-Thylakoid Mutant of Chinese Cabbage (Brassica campestris ssp. pekinensis)

Mutation in EMB1923 gene promoter is associated with chlorophyll deficiency in Chinese cabbage (Brassica campestris ssp. pekinensis)

Identification of two recessive etiolation genes (py1, py2) in pakchoi (Brassica rapa L. ssp. chinensis)

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Screening ofChinese cabbage mutants produced by60Co γ‐ray mutagenesis of isolated microspore cultures

Cited by 30 publications

References 30 publications

Physiological Characterization and Comparative Transcriptome Analysis of a Slow-Growing Reduced-Thylakoid Mutant of Chinese Cabbage (Brassica campestris ssp. pekinensis)

Physiological Characterization and Comparative Transcriptome Analysis of a Slow-Growing Reduced-Thylakoid Mutant of Chinese Cabbage (Brassica campestris ssp. pekinensis)

Mutation in EMB1923 gene promoter is associated with chlorophyll deficiency in Chinese cabbage (Brassica campestris ssp. pekinensis)

Identification of two recessive etiolation genes (py1, py2) in pakchoi (Brassica rapa L. ssp. chinensis)

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Screening ofChinese cabbage mutants produced by⁶⁰Co γ‐ray mutagenesis of isolated microspore cultures