Screening of sepsis using leukocyte cell population data from the Coulter automatic blood cell analyzer DxH800

Abstract

Many of the leukocyte CPD have been identified as useful parameters of sepsis. Hopefully, these parameters can ultimately be incorporated into a decision rule for the screening of sepsis samples and to discriminate fungemia from bacteremia.

Fraction of immature granulocytes reflects severity but not mortality in sepsis

“…Unlike ADViA studies, the clinical studies of iG% were a small number, and showed heterogeneous enrollment and results. The mean value of iG% in a normal population by the Sysmex system has a reported distribution from 0.19-0.22% [21,22] and 95% cut-off values for this normal population ranged from 0.4-0.5% [7,21,23]. in sepsis, iG% cut-off values have a reported distribution of 0.35-0.5% [24,25] and elevation of iG% to more than 2.0-3.0% reported to be specific for the detection of sepsis [18,26,27].…”

“…For this purpose, various inflammatory markers have been proposed as predictors of patient outcome, with C-reactive protein (CRP), lactate and procalcitonin used universally [4,5]. disseminated intravascular coagulation (DiC) and 28-day mortality [6][7][8][9]. immature granuloctyes was comprised of mainly promyelocytes, myelocytes, and metamyelocytes, but not band form neutrophils [10].…”

Biological Variations of Leukocyte Numerical and Morphologic Parameters Determined by UniCel DxH 800 Hematology Analyzer

“…We also evaluated the biological variations of leukocyte morphologic parameters or CPD, mainly because of an increasing number of publications [3][4][5][6][7][8][9][10][11][12][13] in recent years demonstrating the potential clinical utility of CPD in diagnosing many pathologic conditions, particularly in acute bacterial infection or sepsis. [3][4][5][6][7][8][9][10] We showed that the CPD have much smaller overall CV I and CV G compared to numerical parameters, suggesting that these morphologic parameters are less variable around the homeostatic set point intraindividually and interindividually. In addition, the index of individuality for all morphologic parameters was low.…”

“…For example, the neutrophil CPD, such as mean neutrophil volume and neutrophil volume distribution width, are significantly increased not only in septic patients with high WBC, but also in those with normal or low WBC. [3][4][5][6][7] The mean neutrophil volume and/or neutrophil volume distribution width, which reflect respectively neutrophil size and size variations, show superior sensitivity and specificity for predicting sepsis compared to WBC, the percentage of neutrophils, band counts, C-reactive protein, or procalcitonin, proving to be promising indicators for the diagnosis of acute bacterial infection. 8,9 In addition, the mean neutrophil volume and neutrophil volume distribution width are significantly increased in postsurgical patients with bacterial infection compared with noninfected patients, although WBC was increased in both groups.…”

Diagnosis and evaluation of severity of sepsis via the use of biomarkers and profiles of 13 cytokines: a multiplex analysis

“…Among these biomarkers, procalcitonin (PCT) and C-reactive protein (CRP) have been evaluated for use in the diagnosis of sepsis, and were included in the diagnostic criteria for sepsis by the Surviving Sepsis Campaign [6]. Hematologic parameters obtained by automated hematologic analyzer, including white blood cell (WBC) and immature granulocyte (IG) counts, were also evaluated as surrogate markers for sepsis [4,7,8]. As proinflammatory cytokines are released during the acute phase of sepsis, cytokines have been studied as candidate biomarkers for the diagnosis of sepsis.…”