Screening of species-specific lactic acid bacteria for veal calves multi-strain probiotic adjuncts

Ripamonti, B.; Agazzi, Alessandro; Bersani, C.; Dea, P. De; Pecorini, C.; Pirani, S.; Rebucci, R.; Savoini, G.; Stella, S.; Stenico, Alberta; Tirloni, Erica; Domeneghini, C.

doi:10.1016/j.anaerobe.2011.05.001

Cited by 68 publications

(41 citation statements)

References 48 publications

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“…Increased efficacy has been noted in DFMs containing multiple compared with single organisms, possibly due to synergistic mechanisms of action (33), and a DFM including Lactobacillus acidophilus and Propionibacterium freudenreichii has been widely adopted for improvement of growth performance in larger feedlots in the United States (7). Because few reports have identified DFMs that offer concurrent control of pathogens and improved growth performance in feedlot cattle, the primary objective of the current study was to determine the appropriate dosage of a novel DFM containing a mixture of Lactobacillus casei, Lactobacillus lactis, and Paenibacillus polymyxa for reduc tion of Escherichia coli 0157:H7 in feces of feedlot cattle over a complete feeding cycle.…”

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confidence: 97%

“…DFMs are thought to function by a number of mechanisms, including production of inhibitory compounds such as acids or bacteriocins (33), competitive exclusion (7), improvement of rumen fermentation parameters (3), the blocking of quorum sensing (25), improvement of host mucosal immunity (16,34), or by other as yet undefined mechanisms (36). Because the microbial populations of the bovine gastrointestinal tract are exceedingly complex and many interrelationships among organisms are poorly characterized, the efficacy of DFMs has varied (2,39,40).…”

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confidence: 99%

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A Mixture of Lactobacillus casei, Lactobacillus lactis, and Paenibacillus polymyxa Reduces Escherichia coli O157:H7 in Finishing Feedlot Cattle

Stanford

Bach

Baah

et al. 2014

Journal of Food Protection

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A direct-fed microbial (DFM) containing Paenibacillus polymyxa, Lactobacillus casei, and Lactobacillus lactis was fed to cattle (n = 120) to determine impacts on shedding and survival of Escherichia coli O157:H7 in feces. Cattle were individually penned and fed diets containing 0 (control), 4 × 10(7) CFU (DFM-4), 8 × 10(7) CFU (DFM-8), or 1.2 × 10(8) CFU (DFM-12) lactobacilli per kg of dietary dry matter over 84-day fall-winter growing and 140-day spring-summer finishing periods. Fecal grab samples were collected from cattle at 28-day intervals, E. coli O157:H7 was detected by immunomagnetic separation, and isolates were compared by pulsed-field gel electrophoresis. During the growing period, feces negative for E. coli O157 from each dietary treatment were inoculated with 10(5) CFU/g nalidixic acid-resistant E. coli O157:H7 and were incubated at 4 and 22(u) C for 11 weeks. Fecal pH and fecal dry matter were measured on days 0, 1, 3, and 7 and weekly thereafter, with E. coli O157:H7 enumerated through dilution plating. Treatment with DFMs did not affect survival of E. coli O157:H7 in feces or fecal pH (P > 0.05). Only one steer was positive for E. coli O157:H7 during the growing period, but during the finishing period, DFM-8 and DFM-12 reduced the prevalence of E. coli O157:H7 in feces (P < 0.05). Feeding DFMs also reduced the frequency of individual steers shedding E. coli O157:H7 during finishing (P < 0.05), with control steers shedding E. coli O157:H7 up to four times, whereas DFM-12 steers shed E. coli O157:H7 a maximum of twice. Treatment with DFMs influenced pulsed-field gel electrophoresis profiles; steers that were fed DFM-8 and DFM-12 shed more diverse subtypes of E. coli O157:H7 than did control or DFM-4 steers. Because a companion study found linear improvement in performance with increasing dosage of DFMs in the first 28 days of the growing period, targeted use of DFM-12 during this time and for the final 1 or 2 weeks prior to slaughter may optimize performance and reduce E. coli O157:H7 while minimizing feed costs.

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confidence: 97%

mentioning

confidence: 99%

A Mixture of Lactobacillus casei, Lactobacillus lactis, and Paenibacillus polymyxa Reduces Escherichia coli O157:H7 in Finishing Feedlot Cattle

Stanford

Bach

Baah

et al. 2014

Journal of Food Protection

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“…An agar spot test according to Ripamonti et al [2011] was applied to fi nd the ability of selected LAB strains to inhibit a group of foodborne pathogens such as Escherichia coli, Salmonella enterica, Enterococcus hirea, Staphylococcus aureus and Pseudomonas aeruginosa. The mentioned strains originated from the Agricultural Biotechnology Research Institute of Iran (Karaj, Iran).…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Characterization of Probiotic Abilities of Lactobacilli Isolated from Iranian Koozeh Traditional Cheese

Tavakoli

Hamidi‐Esfahani

Hejazi

et al. 2017

Pol. J. Food Nutr. Sci.

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Eight lactic isolates including Lactobacillus plantarum (MT.ZH193, MT.ZH293, MT.ZH393 and MT.ZH593), L. casei (MT.ZH493), L. pentosus (MT.ZH693), and L. fermentum (MT.ZH893 and MT.ZH993) were identifi ed from an Iranian traditional cheese "Koozeh Paneer" using the morphological, phenotypical, biochemical and molecular characterization and then their probiotic characteristics were compared. Results showed that the lactic isolates of L. plantarum (MT.ZH293) and L. fermentum (MT.ZH893 and MT.ZH993) were resistant to all the used bile salts up to concentrations of 0.3 to 2.0%. All the strains showed low sensitivity to the presence of conjugated bile salts. L. plantarum MT.ZH293 exhibited the highest enzymatic activity of β-galactosidase and survival rate in a simulated stomach duodenum passage. L. casei MT.ZH493 generated the highest amount of hydrogen peroxide, followed by L. fermentum MT.ZH993 and L. plantarum MT.ZH593. Although the most selected LAB isolates had a moderate cell surface hydrophobicity, L. plantarum MT. ZH593 expressed the highest cell surface hydrophobicity. L. fermentum MT.ZH893 had strong resistance to all the antibiotics tested such as amoxicillin, ceftriaxon, chloramphenicol, erythromycin, gentamycin, streptomycin, tetracycline and vancomycin.

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“…The isolates were propagated overnight in MRS broth, cells were harvested by centrifugation and 10 9 -10 10 CFU mL −1 were suspended in artificial gastric fluid (NaCl, 0.72 g•L ; pepsin, 0.3 g•L −1 ) adjusted to pH 2.0, 2.5 and 3.0 and incubated for 0, 2 and 4 h. The intestinal juice resistance was studied by exposing the isolates (10 9 -10 10 CFU mL −1 ) to artificial intestinal fluid (0.1% w/v pancreatin and 0.3% w/v bile salts, pH 8.0) for 0, 2 and 4 h [20] [21]. Samples from all trials plated in MRS agar to count colony forming units.…”

Section: Resistance To Simulated Gastric Fluid and Intestinal Fluidmentioning

confidence: 99%

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

Dubey¹,

Jeevaratnam²

2015

AiM

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The in vitro screening of lactobacilli isolates from uttapam batter, fermented supplementing with Piper betle L. leaves, was performed in order to select potent isolates for probiotic use. Their resistance to simulated gastric and intestinal juices as well as their adhesion to epithelial intestinal HCT-15 and vaginal HeLa cell lines were assessed and also evaluated for their immunomodulatory ability in ex vivo condition. Isolates were able to adhere as well as reduce pathogen adhesion to monolayer cell lines' surfaces and reduced production of pro-inflammatory cytokine TNF-α in LPS treated PBMCs, while enhanced that of anti-inflammatory cytokine IL-10. The isolates exhibited properties of auto-aggregation, co-aggregation, hydrophobicity, bile salt hydrolase activity and strong antimicrobial activity against pathogenic bacteria. Thus, the different lactobacilli isolates displayed potent probiotic and immunomodulatory properties among which AJ7 and AJ82 had a great potential and may have applications in fermented foods as immunomodulatory probiotic additives.

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Screening of species-specific lactic acid bacteria for veal calves multi-strain probiotic adjuncts

Cited by 68 publications

References 48 publications

A Mixture of Lactobacillus casei, Lactobacillus lactis, and Paenibacillus polymyxa Reduces Escherichia coli O157:H7 in Finishing Feedlot Cattle

A Mixture of Lactobacillus casei, Lactobacillus lactis, and Paenibacillus polymyxa Reduces Escherichia coli O157:H7 in Finishing Feedlot Cattle

Characterization of Probiotic Abilities of Lactobacilli Isolated from Iranian Koozeh Traditional Cheese

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

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Screening of species-specific lactic acid bacteria for veal calves multi-strain probiotic adjuncts

Cited by 68 publications

References 48 publications

A Mixture of Lactobacillus casei, Lactobacillus lactis, and Paenibacillus polymyxa Reduces Escherichia coli O157:H7 in Finishing Feedlot Cattle

A Mixture of Lactobacillus casei, Lactobacillus lactis, and Paenibacillus polymyxa Reduces Escherichia coli O157:H7 in Finishing Feedlot Cattle

Characterization of Probiotic Abilities of Lactobacilli Isolated from Iranian Koozeh Traditional Cheese

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with &lt;i&gt;Piper betle&lt;/i&gt; L. Leaves

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Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves