Screening of specific binding peptides using phage-display techniques and their biosensing applications

Xu, Ping; Ghosh, Subhadeep; Gul, Anam Rana; Bhamore, Jigna R.; Park, Jong Pil; Park, Tae Jung

doi:10.1016/j.trac.2021.116229

Cited by 48 publications

(23 citation statements)

References 142 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Exogenous peptide was fused into the phage coat protein and displayed on the surface to form combinatorial phage. Phage display establishes physical linkage between peptide/protein and DNA sequence, which allows for rapid separation based on binding affinity with a specific target molecule and facilitates characterization of displayed peptide/proteins after selecting phages with desired binding properties 17 , 19 . Greg Winter and his colleagues applied this technology to therapeutic protein engineering, especially in the discovery and preparation of antibodies.…”

Section: Overview Of Phage Displaymentioning

confidence: 99%

“…Among them, high-throughput screening (HTS) techniques such as phage, mRNA/DNA, yeast, ribosomal, and cell display technologies facilitate the rapid identification of new peptide lead, with phage display being the best understood and most commonly used. Phage display has proved to be predominant platforms for discovery of suitable peptide ligand, including disease-specific or organ-specific peptides 14 , cancer cell/tumor targeting peptides 15 , immune checkpoint antagonists 16 , and biosensors 17 , etc .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phage display derived peptides for Alzheimer's disease therapy and diagnosis

Zhang¹,

Zhang²,

Gao³

et al. 2022

Theranostics

View full text Add to dashboard Cite

Alzheimer's disease (AD) is an incurable and fatal progressive neurodegenerative disorder associated with memory and cognition impairment. AD is one of the top medical care concerns across the world with a projected economic burden of $2 trillion by 2030. To date, however, there remains no effective disease-modifying therapy available. It is more important than ever to reveal novel therapeutic approaches. Peptide-based biotherapeutics has been a great potential strategy attributed to their distinct and superior biochemical characteristics, such as reproducible chemical synthesis and modification, rapid cell and tissue permeability, and fast blood clearance. Phage display, one of today's most powerful platforms, allows selection and identification of suitable peptide drug candidates with high affinities and specificity toward target, demonstrating the potential to overcome challenges and limitations in AD diagnosis/treatment. We aim to provide the first comprehensive review to summarize the status in this research direction. The biological overview of phage display is described, including basic biology of the phage vectors and construction principle of phage library, biopanning procedure, mirror image phage display, and various binding affinity evaluation approaches. Further, the applications of phage display in AD therapy, targeted drug delivery, and early detection are presented. Finally, we discuss the current challenges and offer a future outlook for further advancing the potential application of phage display on AD and other neurodegenerative diseases.

show abstract

Section: Overview Of Phage Displaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phage display derived peptides for Alzheimer's disease therapy and diagnosis

Zhang¹,

Zhang²,

Gao³

et al. 2022

Theranostics

View full text Add to dashboard Cite

show abstract

“…For the selectivity aspect of the collector, biological systems are well known for their molecular recognition capabilities; but this is typically considered with respect to organic ligands. More recently, researchers have demonstrated that phage display techniques can be used to select phage clones that express peptides with a specific binding affinity to a variety of target inorganic materials. ,,,,− ,− In most of the prior studies in this area, the inorganic target is typically a well-defined material, synthesized to high purity, and with homogeneous surface properties. In this study, the inorganic target consisted of ground francolite particles provided by the mining industry, which are inherently heterogeneous in composition and surface properties.…”

Section: Resultsmentioning

confidence: 99%

Biotechnology Approach to Mineral Separation via Phage Flotation Collectors

Liao

Yeh

El‐Shall

et al. 2022

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

A long-standing challenge in the mining industry is the separation of mineral particles that have similar surface characteristics for which surfactant-based flotation collectors cannot discriminate. In Florida phosphate mining, this problem occurs in the separation of dolomite [CaMg(CO3)2] contaminants from the desired francolite mineral {a fluorapatite [Ca5(PO4)3(F,OH)]}. In this study, phage display techniques were used to select phage clones with specific binding affinity to francolite, which were then tested in a benchtop bubbler flotation apparatus for their ability to selectively float francolite particles from mixtures containing dolomite. Contact angles measured with the captive bubble technique were used to examine changes in the surface character of the mineral particles upon adsorption of the phage, which showed that the most selective phage led to an increase in the contact angle from 16 to 50°. Although this is below the level considered hydrophobic, the correlation between contact angles and increased flotation recovery suggests that the phage coat proteins are behaving as efficient bioamphiphiles for the attachment of the particles to air bubbles, demonstrating a new and environmentally friendly type of biocollector system. The chemical and physical characteristics of the phage “tail” peptides were evaluated to offer an explanation for the specificity of phage binding. We conclude with a discussion of the potential benefits of this biotechnology approach, even for commodity industries such as mining or other particle separation systems, when costs and sustainability are considered.

show abstract

“…In comparison with other biological recognition elements such as antibodies or aptamers, phages are cheaper, very specific, and easy to modify and to handle even if under severe experimental conditions [ 167 , 168 , 169 , 170 ].…”

Section: Electrochemical Biosensors For Food Allergen Detectionmentioning

confidence: 99%

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Curulli

2022

Biosensors

View full text Add to dashboard Cite

Food allergy has been indicated as the most frequent adverse reaction to food ingredients over the past few years. Since the only way to avoid the occurrence of allergic phenomena is to eliminate allergenic foods, it is essential to have complete and accurate information on the components of foodstuff. In this framework, it is mandatory and crucial to provide fast, cost-effective, affordable, and reliable analysis methods for the screening of specific allergen content in food products. This review reports the research advancements concerning food allergen detection, involving electrochemical biosensors. It focuses on the sensing strategies evidencing different types of recognition elements such as antibodies, nucleic acids, and cells, among others, the nanomaterial role, the several electrochemical techniques involved and last, but not least, the ad hoc electrodic surface modification approaches. Moreover, a selection of the most recent electrochemical sensors for allergen detection are reported and critically analyzed in terms of the sensors’ analytical performances. Finally, advantages, limitations, and potentialities for practical applications of electrochemical biosensors for allergens are discussed.

show abstract

Screening of specific binding peptides using phage-display techniques and their biosensing applications

Cited by 48 publications

References 142 publications

Phage display derived peptides for Alzheimer's disease therapy and diagnosis

Phage display derived peptides for Alzheimer's disease therapy and diagnosis

Biotechnology Approach to Mineral Separation via Phage Flotation Collectors

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Contact Info

Product

Resources

About