Screening of specific diagnostic peptides of swine hepatitis E virus

Zhao, Kai; Liu, Qiwen; Yu, Ran; Li, Zhen; Jian-yue, LI; Hong, Zhu; Wu, Ximei; Tan, Furong; Wang, Jinbin; Tang, Xueming

doi:10.1186/1743-422x-6-186

Cited by 15 publications

(16 citation statements)

References 26 publications

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“…The peptide segments identified from the set of 98 sequences of the SARS-CoV-2 S glycoprotein appear to hold reasonable potential to act as immunogens. Peptide-based diagnostics and vaccines have been proposed against virus outbreaks earlier 29-33 . The availability of a 3D structure (6VSB) of the SARS-CoV-2 S glycoprotein provided an opportunity to inspect the predicted peptides.…”

Section: Resultsmentioning

confidence: 99%

Understanding the B and T cells epitopes of spike protein of severe respiratory syndrome coronavirus-2: A computational way to predict the immunogens

Vashi

Jagrit

Kumar

2020

Preprint

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24The 2019 novel severe respiratory syndrome coronavirus-2 (SARS-CoV-2) outbreak 25 has caused a large number of deaths with thousands of confirmed cases worldwide. The present 26 study followed computational approaches to identify B-and T-cell epitopes for spike 27 glycoprotein of SARS-CoV-2 by its interactions with the human leukocyte antigen alleles. We 28 identified twenty-four peptide stretches on the SARS-CoV-2 spike protein that are well 29 conserved among the reported strains. The S protein structure further validated the presence of 30 predicted peptides on the surface. Out of which twenty are surface exposed and predicted to 31 have reasonable epitope binding efficiency. The work could be useful for understanding the 32 immunodominant regions in the surface protein of SARS-CoV-2 and could potentially help in 33 designing some peptide-based diagnostics. 34 35 36 37 38 39 40 41 42 43 44 45 46 47Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a recent 51 pandemic and declared as a public health emergency by World Health Organization (WHO) 1 . 52The disease rapidly spread across the globe and caused havoc to humanity 2 . By the end of 53 March, SARS-CoV-2 had spread to 200 countries and infected over 4,50,000 people 3 . The 54 WHO is continuously monitoring and updating the health-related plans to curtail the disease 55 spread. The absence of specific treatment and vaccine worsen the situation and threat the world. 56 International Committee on Taxonomy of Viruses (ICTV), classified SARS-CoV-2 57 under family coronaviridae of order nidovirales. The genomic sequence of SARS-CoV-2 58 isolated from the bronchoalveolar lavage fluid of a patient from the Wuhan, China showed a 59 length of 29,903 nucleotides (GenBank accession number NC_045512) 4 . The SARS-CoV-2 60 contains a positive single-stranded RNA with 5ˊ and 3ˊ UTR. The genome codes for ORF1a, 61 ORF1b, Spike (S), ORF3a, ORF3b, Envelope (E), Membrane (M), ORF6, ORF7a, ORF7b, 62ORF8, ORF9b, ORF14, Nucleocapsid (N), and ORF10 from 5ˊ to 3ˊ 4,5 . 63The S glycoprotein forms homotrimers and represents a potential target for therapeutic 64 and vaccine design as it mediates viral entry into host cells 6,7 . S glycoprotein comprises of two 65 functional subunits. Whereas the S1 subunit is responsible for binding to the host cell receptor, 66 the S2 subunit is responsible for the fusion of the viral with the cell membrane. Usually, in 67 CoVs, S is cleaved at the boundary between the S1 and S2 subunits, which remain non-68 covalently bound in the prefusion conformation, to activate the protein for membrane fusion 69 via extensive irreversible conformational changes [8][9][10] . Setting apart from other SARS-CoVs, it 70 is found that S glycoprotein of SARS-CoV-2 harbors a furin cleavage site at the boundary 71 between the S1/S2 subunits 11 . By now, it is evident that SARS-CoV-2 S uses angiotensin-72 converting enzyme 2 (ACE2) receptor-mediated entry into cells. It is found that the receptor-73 was not certified by peer review) is th...

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Section: Resultsmentioning

confidence: 99%

Understanding the B and T cells epitopes of spike protein of severe respiratory syndrome coronavirus-2: A computational way to predict the immunogens

Vashi

Jagrit

Kumar

2020

Preprint

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“…Currently, different synthetic peptides and recombinant antigens of ORF2 and 3 of genotypes 1, 2, and 3 are used in serological diagnostics of human and animal HEV infections (Osterman et al 2012;Riddell et al 2000;Pezzoni et al 2014;Wang et al 2017). This could, to some extent, be explained by the fact that major antigenic epitopes of HEV are located more in the ORF2 (Wang et al 2001) and ORF3 (Zhao et al 2009) regions than in the ORF1 coding region (Osterman et al 2012). Further studies characterizing the immunogenic properties of the ORF2-and ORF3-based recombinant virus proteins showed that ORF2 polyprotein, and especially its Cterminus part (O2C or p166 protein), is characterized by the highest immunogenicity among all tested virus antigens so far (Liang et al 2010;Osterman et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Application of ELISA recomWell HEV IgG (Human) for Detection of Virus-Specific Antibodies in Sera of Slaughtered Rabbits

Bigoraj

Rzeżutka

2018

Food Anal. Methods

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HEV infections in animals are mainly diagnosed based on the presence of anti-HEV antibodies in sera using enzyme-linked immunosorbent assays (ELISA). In fact, commercial HEV ELISA kits are only available for selected animal species excluding rabbits. The demonstration of cross-reactivity of rabbit anti-HEV antibodies with human HEV strains makes possible diagnostic use of (human) ELISA kits for detection of specific anti-HEVantibodies in rabbit sera. The aim of the study was the application of ELISA recomWell HEV IgG (human) for identification of HEV seropositive rabbits at the time of slaughter. Native recombinant protein A was used as broadly reactive conjugate characterized by cross-species reactivity with IgG antibodies of human and other animal species. Several dilutions of protein A were employed and subsequently tested against the panel of control sera (positive, negative, and borderline) to determine its optimal concentration. Statistical analysis of OD values and their corresponding antibody titers obtained for each group of tested sera and protein A showed the most consistent performances at 1:40,000 dilution when compared to anti-human IgG (control) conjugate. Finally, the correct performance of the optimized test was verified using rabbit serum samples containing (or not) specific anti-HEV antibodies. As demonstrated here, HEV IgG (human) ELISA employing ORF2 antigens of human gt1 and gt3 HEV strains after modification and subsequent optimization can be used for the detection of anti-HEV antibodies in rabbit sera.

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“…ORF3 encodes a 113–114 residue multifunctional protein required for activating extracellularly regulated kinase [ 25 ], releasing virus, facilitating infection [ 26 ] and increasing expression of glycolytic enzymes [ 27 ]. Earlier investigations strongly suggest that a number of ORF3 peptides have good antigenicity and are highly sensitive [ 28 ], supporting the utility of ORF3 as a potential candidate for detecting specific antibodies against HEV in serum samples.…”

Section: Introductionmentioning

confidence: 92%

A Proline-Rich Domain in the Genotype 4 Hepatitis E Virus ORF3 C-Terminus Is Crucial for Downstream V105DLP108 Immunoactivity

Wang

Liang

et al. 2015

PLoS ONE

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The hepatitis E virus (HEV) is responsible for serious viral hepatitis worldwide. Animals are considered a reservoir of HEV, particularly pigs. While HEV infection in pigs and dogs is always asymptomatic, the virus causes high death rates in patients with pre-existing chronic liver disease and pregnant women in developing countries. HEV open reading frame 2 (ORF2) has been used as a diagnostic target to detect specific antibodies against HEV in serum samples. Recent research has additionally supported the potential utility of the ORF3 protein as a target in serum anti-HEV detection. However, the epitope distribution of ORF3 protein remains ambiguous. In the current study, we showed that continuous amino acid motif, VDLP, at the C-terminus of genotype 4 HEV ORF3 is a core sequence of the ORF3 protein epitope. Moreover, cooperative interaction with upstream elements is essential for its immunoactivity. Three proline residues (P99, P102 and P103) in the upstream proline-rich domain exerted significant effects on the immunocompetence of VDLP. ELISA results revealed that SAPPLPPVVDLP and SAPPLPPVVDLPQLGL peptides containing the identified VDLP epitope display weaker reactions with anti-HEV serum than the commercial ELISA kit. Our collective findings provide valuable information on the epitope distribution characteristics of HEV ORF3 and improve our understanding of the influence of the proline-rich domain on the immunoactivity of downstream amino acids in the C-terminal region.

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Screening of specific diagnostic peptides of swine hepatitis E virus

Cited by 15 publications

References 26 publications

Understanding the B and T cells epitopes of spike protein of severe respiratory syndrome coronavirus-2: A computational way to predict the immunogens

Understanding the B and T cells epitopes of spike protein of severe respiratory syndrome coronavirus-2: A computational way to predict the immunogens

Application of ELISA recomWell HEV IgG (Human) for Detection of Virus-Specific Antibodies in Sera of Slaughtered Rabbits

A Proline-Rich Domain in the Genotype 4 Hepatitis E Virus ORF3 C-Terminus Is Crucial for Downstream V105DLP108 Immunoactivity

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