Screening of Spirulina strains for high copper adsorption capacity through Fourier transform infrared spectroscopy

Liu, Jinghua; Zhu, Chen; Li, Zhengpeng; Zhou, Haoyuan

doi:10.3389/fmicb.2022.952597

Cited by 3 publications

(2 citation statements)

References 28 publications

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“…However, peaks at 2040 and 3109 cm –1 were noticed in the cultivated biomass in the absence of Cl – . These peaks may be assigned to CHCH– of carotenoid and alkynes having structure CC (triple bond). The main components determined were carbohydrates, lipids, and pigments under both cultivation conditions.…”

Section: Resultsmentioning

confidence: 99%

Unlocking the Potential: Algal Biomass Cultivation and Growth Kinetics Using Tanning Process Water

Gagrai

2024

ACS Sustainable Resour. Manage.

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A basic kinetic model has been applied to microalgae to predict the growth parameters in Zarrouk Media composition (ZMC) and precipitated chrome tanning effluent (PCTE). The model was fitted with the experimental data of Spirulina cultivation to estimate growth parameters: nutrient adsorption constant (K a) (h–1), nutrient desorption constant (K d) (h–1), rate of respiration (rR c)(h–1), efficiency of bio-synthesis (β) (g g–1), respiration rate (h–1)(rR c), rate of maximum photosynthesis (p max) (h–1), coefficient for light-absorption (α) (m2 g–1), photon efficiency (g.μmol–1 photons–1) (φm), etc. The model suggests a higher nutrient adsorption rate in ZMC (0.75 h–1) as compared to PCTE (1.40 × 10–2 h–1). The rate of respiration of Spirulina decreased due to cultivation in PCTE from 5.36 × 10–3 to 1.91 × 10–3 h–1. The biosynthetic efficiency of Spirulina decreased from 8.72 to 2.002 due to cultivation in PCTE media. The maximum photosynthetic rate h–1 was slightly higher in ZMC as compared to PCTE media. The model parameter values were lower for Spirulina in PCTE than those in ZMC. Spirulina’s cell density was lower in PCTE compared to ZMC, as the doubling time was increased from 9.97 h–1 to 31.47 h–1. Moreover, the optimum pH for growth was also shifted from 9.5 to 10.5. The higher dose of PCTE (Cl– > 2.96 × 103 mg L–1) restricted cell growth. Adding Cr(III) in ZMC has a higher impact on cell growth than Cr(VI). The model parameters with Cr(III) and Cr(VI) also showed a decrease in values except rRc and β have higher values for Cr(VI) as compared to Cr(III) added ZMC due to the non-interaction of Cr(VI) with algae. The model-predicted cell growth rates closely align with experimental results, with deviations within an ±7% margin. The addition of heavy metals to ZMC disrupts nutrient interactions and transport mechanisms during Spirulina cell growth.

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Section: Resultsmentioning

confidence: 99%

Unlocking the Potential: Algal Biomass Cultivation and Growth Kinetics Using Tanning Process Water

Gagrai

2024

ACS Sustainable Resour. Manage.

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“…Additionally, FTIR spectroscopy can be performed to identify microalgae components (e.g., lipids, proteins, carbohydrates, and carotenoids) and further explore the mechanism of adsorption and degradation of environmental pollutants, owing to its distinctive advantages of simplicity, high efficiency, and non-destruction ( Zhang et al, 2020 ; Liu et al, 2022 ). For instance, Pelusi et al (2016) discovered a characteristic absorption peak due to the stretching vibration of -C=C group of alkenones at 962.5 cm −1 in Emiliania huxleyi .…”

Section: Discussionmentioning

confidence: 99%

Toxicity, physiological response, and biosorption mechanism of Dunaliella salina to copper, lead, and cadmium

Gao,

Ling,

Tian

et al. 2024

Front. Microbiol.

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BackgroundHeavy metal pollution has become a global problem, which urgently needed to be solved owing to its severe threat to water ecosystems and human health. Thus, the exploration and development of a simple, cost-effective and environmental-friendly technique to remove metal elements from contaminated water is of great importance. Algae are a kind of photosynthetic autotroph and exhibit excellent bioadsorption capacities, making them suitable for wastewater treatment.MethodsThe effects of heavy metals (copper, lead and cadmium) on the growth, biomolecules accumulation, metabolic responses and antioxidant response of Dunaliella salina were investigated. Moreover, the Box-Behnken design (BBD) in response surface methodology (RSM) was used to optimize the biosorption capacity, and FT-IR was performed to explore the biosorption mechanism of D. salina on multiple heavy metals.ResultsThe growth of D. salina cells was significantly inhibited and the contents of intracellular photosynthetic pigments, polysaccharides and proteins were obviously reduced under different concentrations of Cu2+, Pb2+ and Cd2+, and the EC50 values were 18.14 mg/L, 160.37 mg/L and 3.32 mg/L at 72 h, respectively. Besides, the activities of antioxidant enzyme SOD and CAT in D. salina first increased, and then descended with increasing concentration of three metal ions, while MDA contents elevated continuously. Moreover, D. salina exhibited an excellent removal efficacy on three heavy metals. BBD assay revealed that the maximal removal rates for Cu2+, Pb2+, and Cd2+ were 88.9%, 87.2% and 72.9%, respectively under optimal adsorption conditions of pH 5-6, temperature 20-30°C, and adsorption time 6 h. Both surface biosorption and intracellular bioaccumulation mechanisms are involved in metal ions removal of D. salina. FT-IR spectrum exhibited the main functional groups including carboxyl (-COOH), hydroxyl (-OH), amino (-NH2), phosphate (-P=O) and sulfate (-S=O) are closely associated with the biosorption or removal of heavy metalsions.DiscussionAttributing to the brilliant biosorption capacity, Dunaliella salina may be developed to be an excellent adsorbent for heavy metals.

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Biosorption and bioreduction of aqueous chromium (VI) by different Spirulina strains

Meng

Liu

et al. 2023

FEMS Microbiology Letters

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Spirulina has emerged as a promising microorganism for the treatment of industrial heavy metal ions in wastewater due to their simplicity of cultivation and harvesting, rich functional binding groups and high bioreductive activity during the uptake process. While the capacities of biosorption and bioreduction for heavy metal ions differs significantly among various algal strains. Therefore, the physiological characteristics were investigated to identify the different Spirulina strains and the chromium (VI) adsorption capacities of the algal strains were also evaluated. In this study, it was found that algal strains YCX2643 and CLQ1848 performed higher removal efficiency (86.5% and 83.7%) than other four Spirulina strains (59.4%, 56.3%, 65.6% and 66.5%, respectively). Moreover, the mechanisms of chromium (VI) ions binding and biotransformation in the Spirulina cell were scrutinize by FT-IR spectroscopy and scanning electron microscopy(SEM), and it indicated that the varieties of cellular components involved in high binding affinity may cause the higher biosorption and bioreduction of aqueous chromium (VI) in algal strains YCX2643 and CLQ1848, which could be used as promising biosorbents in the removing heavy metal pollutants from wastewaters.

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Screening of Spirulina strains for high copper adsorption capacity through Fourier transform infrared spectroscopy

Cited by 3 publications

References 28 publications

Unlocking the Potential: Algal Biomass Cultivation and Growth Kinetics Using Tanning Process Water

Unlocking the Potential: Algal Biomass Cultivation and Growth Kinetics Using Tanning Process Water

Toxicity, physiological response, and biosorption mechanism of Dunaliella salina to copper, lead, and cadmium

Biosorption and bioreduction of aqueous chromium (VI) by different Spirulina strains

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