Screening of tetrodotoxin in puffers using gas chromatography–mass spectrometry

Man, Che Nin; Noor, Norjuliana Mohd; Harn, Gam Lay; Lajis, Razak; Mohamad, Samsur

doi:10.1016/j.chroma.2010.09.064

Cited by 31 publications

(15 citation statements)

References 29 publications

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“…Surface plasma resonance [136], electrophysiological assays [97,98,191,232]; IR [96]; NMR [53,59,96,125,173,241,242]; GC-MS [7,8,85,137,139,169,170,171]; LC-FLD and LC-MS (Table 4, Table 5, Table 6, Table 7, Table 8 and Table 9) have been developed and used for the determination of TTX by many researchers. In addition NMR, GC-MS, LC-FLD and LC-MS provide the benefit of finding not only TTX but related analogues along with co-occurring toxins in samples thus allowing a more comprehensive risk assessment of produce intended for human consumption.…”

Section: Historical Perspective On Analytical Methods Used For Ttmentioning

confidence: 99%

“…Voglibose has a similar structure that of TTX and also had similar chromatographic and MS properties, so it is a good IS for TTX analysis. Man et al ., 2010 [137] used salicylic acid as the internal standard. …”

Section: Historical Perspective On Analytical Methods Used For Ttmentioning

confidence: 99%

“…Its structure was elucidated by R. B. Woodward in 1964 [133]. Authentic standards for TTX are available from various commercial sources (Table 2); Wako Chemicals (Purity >90%) [134], Latoxan (Valence, France) [43], Sigma Aldrich (St. Louis, MO, USA), 99% HPLC [104,135], Sankyo Co., Ltd, Tokyo, Japan [136], Calbiochem, EMD Millipore, USA, [51], Ascent Scientific [137] and Axxora, Grunberg, Germany [138]. …”

Section: Structures and Standards For Ttx And Its Analoguesmentioning

confidence: 99%

See 2 more Smart Citations

Tetrodotoxin: Chemistry, Toxicity, Source, Distribution and Detection

Bane

Lehane

Dikshit

et al. 2014

Toxins

325

286

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Tetrodotoxin (TTX) is a naturally occurring toxin that has been responsible for human intoxications and fatalities. Its usual route of toxicity is via the ingestion of contaminated puffer fish which are a culinary delicacy, especially in Japan. TTX was believed to be confined to regions of South East Asia, but recent studies have demonstrated that the toxin has spread to regions in the Pacific and the Mediterranean. There is no known antidote to TTX which is a powerful sodium channel inhibitor. This review aims to collect pertinent information available to date on TTX and its analogues with a special emphasis on the structure, aetiology, distribution, effects and the analytical methods employed for its detection.

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Section: Historical Perspective On Analytical Methods Used For Ttmentioning

confidence: 99%

Section: Historical Perspective On Analytical Methods Used For Ttmentioning

confidence: 99%

Section: Structures and Standards For Ttx And Its Analoguesmentioning

confidence: 99%

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Tetrodotoxin: Chemistry, Toxicity, Source, Distribution and Detection

Bane

Lehane

Dikshit

et al. 2014

Toxins

325

286

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“…neurotoxin (Berry and Hassan, 1973;Chulanetra et al, 2011;Man et al, 2010;Ngy et al, 2008). Although, in Japan and Korea, poisonous puffer fish is considered a national delicacy, in other countries including Thailand, the use of puffer fish in food products is entirely prohibited (Nader et al, 2012).…”

Section: Identification Of Puffer Fish Of the Genus Lagocephalus 217mentioning

confidence: 97%

Identification of Puffer Fish of the GenusLagocephalus: L. lunaris, L. spadiceusandL. inermis, Using Multiplex PCR

2014

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A multiplex PCR assay was developed to identify widespread poison-containing puffer fishes of the genus Lagocephalus: L. lunaris, L. spadiceus and L. inermis, using a novel KUGEN_ILSspec primer set which is specific for 12S ribosomal RNA (12S rRNA) and Cytochrome b (Cyt b) genes. KUGEN_ILSspec set is composed of fish positive-amplified primers that produced a 289-bp fragment while L. lunaris-, L. spadiceus-and L. inermis-specific primers produced 123-, 196-and 493-bp fragments, respectively. The sensitivity of this primer set was found to be high as it was capable of amplifying targeted DNA at concentrations as low as 1 ng/µL. Moreover, the primer set was shown to amplify intact DNA and species-specific fragments from heat-processed food. Consequently, multiplex PCR technique in combination with KUGEN_ILSspec primer set could offer an effective measure for detecting poisonous puffer fish contamination in both fresh and processed fish products, which will greatly aid in public health control and law enforcing endeavors.

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“…), almost the same as those from the TMS-C9 base derived from authentic TTX (Figure 12). Screening of tetrodotoxin in pufferfish using GC-MS was reported [41]. Sensitive analysis of TTX in human plasma by solid-phase extraction and GC-MS was reported [42].…”

Section: Mass Spectrometry 41 Gas-chromatography-mass Spectrometrymentioning

confidence: 99%