Screening of the active Ingredients in Huanglian Jiedu decoction through amide bond-Immobilized magnetic nanoparticle-assisted cell membrane chromatography

Liao, Fengyun; Dan, He; Vong, Chi Teng; Wang, Lisheng; Chen, Zhangmei; Zhang, Tiejun; Luo, Hua; Wang, Ying

doi:10.3389/fphar.2022.1087404

Cited by 3 publications

(1 citation statement)

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“…2024, 25, 1153 2 of 14 years, there has been a discernible rise in the development of novel CMSP materials with the specific objective of screening active constituents in traditional Chinese medicine. Particularly, cell membrane chromatography has made substantial progress in evaluating the efficacy of TCM by improving protein carrier and enhancing the binding of proteins with carriers [8][9][10]. Consequently, there has been a significant surge in research endeavors focused on the interaction between silica gel carriers and proteins, leading to the emergence of various innovative techniques that facilitate this process.…”

Section: Introductionmentioning

confidence: 99%

Establishment of Polydopamine-Modified HK-2 Cell Membrane Chromatography and Screening of Active Components from Plantago asiatica L.

Gao,

Liu,

Song

et al. 2024

IJMS

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Cell membrane chromatography (CMC) has been widely recognized as a highly efficient technique for in vitro screening of active compounds. Nevertheless, conventional CMC approaches suffer from a restricted repertoire of cell membrane proteins, making them susceptible to oversaturation. Moreover, the binding mechanism between silica gel and proteins primarily relies on intermolecular hydrogen bonding, which is inherently unstable and somewhat hampers the advancement of CMC. Consequently, this investigation aimed to establish a novel CMC column that could augment protein loading, enhance detection throughput, and bolster binding affinity through the introduction of covalent bonding with proteins. This study utilizes polydopamine (PDA)-coated silica gel, which is formed through the self-polymerization of dopamine (DA), as the carrier for the CMC column filler. The objective is to construct the HK-2/SiO2-PDA/CMC model to screen potential therapeutic drugs for gout. To compare the quantity and characteristics of Human Kidney-2 (HK-2) cell membrane proteins immobilized on SiO2-PDA and silica gel, the proteins were immobilized on both surfaces. The results indicate that SiO2-PDA has a notably greater affinity for membrane proteins compared to silica gel, resulting in a significant improvement in detection efficiency. Furthermore, a screening method utilizing HK-2/SiO2-PDA/CMC was utilized to identify seven potential anti-gout compounds derived from Plantago asiatica L. (PAL). The effectiveness of these compounds was further validated using an in vitro cell model of uric acid (UA) reabsorption. In conclusion, this study successfully developed and implemented a novel CMC filler, which has practical implications in the field.

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Section: Introductionmentioning

confidence: 99%