Screening of the Antioxidant Activity of Crude Extracts in 86 Algae Species From El Jadida Coast (Morocco)

Abstract

Objective: This work aimed to screen the antioxidant activity of marine macroalgae from the Moroccan Atlantic coast (region of El Jadida).
 Methods: Evaluation of the antioxidant activity of different collected species, lyophilized and extracted with a solvent mixture chloroform/methanol (2/1; v/v) was conducted according to two techniques, first by thin layer chromatography (tlc) then by spectrophotometry, using a free radical 2,2-diphenyl-1-picrylhydrazyl (dpph). The sampling on a distance of 110 km allowed to harvest 86 algal species (16 brown algae, 47 red algae, 14 green algae and 9 algae being identified).
 Results: The analysis by thin layer chromatography reveals an antioxidant activity in nearly half of harvested algal species (52.32 %). This activity varies depending on the concentration of the extract and in function of incubation time in the presence of dpph. The monitoring of the kinetics of degradation of dpph by spectrophotometer in the presence of extracts which were active by tlc allowed to confirm the results and select the most active algal species based on the percentage of remaining dpph in the medium after 120 min of reaction: Fucus spiralis (17.02 %), Cyctoseira ericoides (12.16 %) (Phaeophyceae), and Gracilaria multipartita (36%), Halopitys incurvus (5%) (Rhodophyceae).
 Conclusion: The results show that the methodology adopted in this work is reliable and can be used for rapid screening of antioxidant property in plants and the species: Fucus spiralis, Cyctoseira ericoides, Gracilaria multipartita, and Halopitys incurvus can be a promising source of natural compounds endowed with high antioxidant potential.

First Report of Halopithys incurva (Rhodomelaceae, Rhodophyta) from the Marchica Lagoon of Nador (North-Est Morocco, Mediterranean)

“…On the Moroccan Mediterranean coast, Halopithys incurva has been recorded in Saaidia, Mellila, Tetouan, Ksar Srgair (Tanger) [51], and localities in the Atlantic Ocean, such as Tangier, Rabat, Mohammedia, Casablanca, Essaouira, Agadir [51] and Sidi Bouzid to El Jadida [55]. According to Boudouresque [56], H. incurvus often forms very dense populations that exclude most large algae.…”

“…Halopithys incurva plays an important role in several fields, such as chemistry. It is richer in chemical compounds, namely, arsenic, manganese and brominated compounds [13,14], is rich in halogenated E3S Web of Conferences 527, 01002 (2024) https://doi.org/10.1051/e3sconf/202452701002 JOE4 secondary metabolites [14,15] and, in particular, is rich in polyphenols with high antioxidant power [15]. Halopithys incurva acted as a good inhibitor of carbon steel corrosion in acid [16].…”

Optimization of Seaweed Harvesting for Maximum Antimicrobial Activity: Impact of Seasonal Variation, Temperature, and Salinity

“…In recent years, several studies have been conducted to investigate the antimicrobial activity of marine algae from the Moroccan coast. However, only a few species have been examined in depth (Aamiri et al, 2023;Bahammou et al, 2021;Boujaber et al, 2016;Chibi et al, 2019;Ibtissam et al, 2009).…”

Insight into corrosion inhibition mechanism of carbon steel by an algal extract as an eco‐friendly corrosion inhibitor in 0.5 M H2SO4: Experimental and molecular dynamics study

“…It is already known for its antibacterial, antiviral, antiparasitic, and antioxidant effects. [14,[15][16][17] The chemical composition of this algae is not very detailed in the literature, and the number of realized works is rather limited. In terms of secondary metabolite, brominated phenolic compounds remain the most abundant.…”