Screening of the in vitro amoebicidal activities of Pastinaca armenea (Fisch. & C.A.Mey.) and Inula oculus-christi (L.) on Acanthamoeba castellanii cysts and trophozoites

Abstract

The aim of this study is to evaluate the in vitro amoebicidal activity of the aqueous extracts of Pastinaca armenea and Inula oculus-christi from Turkey. In the presence of aqueous extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoeba castellanii trophozoites and cysts were decreased during the experimental process. Both extracts showed a time- and dose-dependent amoebicidal action on the trophozoites and cysts. Among the extracts tested, I. oculus showed the strongest amoebicidal effect on the trophozoites and cysts. In the presence of 32.00 mg/ml extract solution in the media, no viable trophozoites were determined from the time of 24 h. Moreover, in the presence of 16.00 mg/ml I. oculus-christi extract, no viable trophozoites were detected in 72 h of the experiment. Effectiveness of I. oculus-christi extract was found moderate against the cysts. In the presence of 32.00 mg/ml extract, only 25.3% of the total cysts were killed effectively. In the case of P. armenea, 40.3% and 23.0% of the trophozoites and cysts were killed in the presence of 32.00 mg/ml extract at the end of the experimental process (72 h), respectively.

Amoebicidal activity of the rhizomes and aerial parts of Allium sivasicum on Entamoeba histolytica

“…These include antibiotics as well as antifungal, antiviral, antiprotozoal, antipsychotic, and anticancer agents (Mattana et al 2004;Osato et al 1991;Schuster and Visvesvara 1998;Walochnik et al 2002). Amebicidal activities of some plant species have previously been reported by our research team (Tepe et al 2011Goze et al 2009;Akin Polat et al 2007a, b, 2008Malatyali et al 2012a, b;Degerli et al 2012).…”

“…In this context, amebicidal activities of some plant species have been reported by our research team (Tepe et al 2011Goze et al 2009;Akin Polat et al 2007a, b, 2008Malatyali et al 2012a, b;Degerli et al 2012). Drugs of natural origin have already been used to treat other parasitic diseases (Arrieta et al 2001;Kayser et al 2003;Said Fernández et al 2005).…”

In vitro amoebicidal activities of Satureja cuneifolia and Melissa officinalis on Acanthamoeba castellanii cysts and trophozoites

“…These include antibiotics as well as antifungal, antiviral, antiprotozoal, antipsychotic, and anticancer agents (Mattana et al 2004;Osato et al 1991;Schuster and Visvesvara 1998;Walochnik et al 2002). On the other hand, amoebicidal activities of some plant species have previously been reported by our research team (Tepe et al 2011a, b;Goze et al 2009;Akin Polat et al 2007a, b, 2008Degerli et al 2011;Malatyali et al 2011).…”

Anatomical and micro-morphological analysis of the fruit and vegetative organs of Inula oculus-christi L. in the Pannonian part of Serbia

“…It was recorded at the site of Rimski Šanac, which is threatened by anthropogenic activities. I. oculuschristi is well documented to have cytotoxic effects (Mossaddegh et al, 2006), amoebicidal effects (Degerli et al, 2011), and antioxidant, antimicrobial and DNA damage protection properties (Berk et al, 2011). However, anatomical and micro-morphological features of I. oculuschristi have not been documented so far.…”