Screening of Toxoplasma Gondii Infection among Childbearing Age Females and Assessment of Nurses' Role in Prevention and Control of Toxoplasmosis

Saleh, Ahmed; Ali, Hisham Abd El-Raouf; AHMED, SALWA; Hosny, Samah Mostafa; Morsy, Tosson A

doi:10.12816/0006471

Cited by 25 publications

(18 citation statements)

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“…In relation to nurses' attitude toward toxoplasmosis during pregnancy, the findings of the present study illustrated that more than half of nurses had negative attitude toward toxoplasmosis. This result disagreed with Saleh et al, (2014) who studied that screening of toxoplasma gondii infection among childbearing age females and assessment of nurses' role in prevention and control of toxoplasmosis and mentioned that almost half of the nurses had positive attitude toward toxoplasmosis.…”

Section: Discussioncontrasting

confidence: 67%

“…While, the finding of the present study revealed that more than two thirds of nurses didn't expect that infection control measures are important for preventing transmission of toxoplasmosis. This result disagreed with Saleh et al, (2014) who studied that screening of toxoplasma gondii infection among childbearing age females and assessment of nurses' role in prevention and control of toxoplasmosis and mentioned that almost half of the nurses had satisfactory levels of knowledge to toxoplasmosis infection control measures. Moreover, adherence to strict infection prevention measures was a must to eliminate exposure to toxoplasmosis infection.…”

Section: Discussioncontrasting

confidence: 59%

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Nurses´ Expectations toward Toxoplasmosis during Pregnancy

El-Rahaman

shabana

Omran

et al. 2017

Egyptian Journal of Health Care

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The protozoan parasite Toxoplasma gondii (T. gondii) causes infection in humans all around the world .Infection with T. gondii is often acquired by ingesting food or water contaminated with oocyst shed by cats or by eating raw or undercooked meat containing tissue cysts. AIM: Aim of the study was to investigate nurses' expectations toward toxoplasmosis during pregnancy. Setting: the study was conducted at obstetric department at Benha University and Benha Teaching hospital. Design: was descriptive. Sampling Type: Convenient sample consisted of 110 nurses. Tools of Data Collection: three tools were used for collecting data; Structured interview questionnaire, likert scale and expectation tool. Results: more than two thirds of the studied nurses had incorrect knowledge toward toxoplasmosis during pregnancy, nearly half of the studied nurses had not expected and more than half of the studied nurses had negative attitude toward toxoplasmosis during pregnancy. The present study findings illustrated that there was a positive statistically significant correlation between total expectations scores, total attitude and total knowledge. Conclusion& Recommendations: the majority of nurses had incorrect knowledge and negative attitude toward toxoplasmosis during pregnancy. Continuous preservice, in service and on job training should be designed to improve nurses` knowledge, expectations and attitude.

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Section: Discussioncontrasting

confidence: 67%

Section: Discussioncontrasting

confidence: 59%

Nurses´ Expectations toward Toxoplasmosis during Pregnancy

El-Rahaman

shabana

Omran

et al. 2017

Egyptian Journal of Health Care

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“…Diagnosis of toxoplasmosis by detecting cysts in tissue is very difficult. So, the detection of Toxoplasma-antibody response by particularly by IHAT for man and animals was accepted as a dependable tool for diagnosis (Saleh et al, 2014). The present results went with the fact that T. gondii infection neither by direct microscopy nor by meat homogenization.…”

Section: Introductionsupporting

confidence: 74%

Parasites Transmitted to Human by Ingestion of Different Types of Meat, El-Minia City, El-Minia Governorate, Egypt

Abdel-Hafeez

Kamal

Abdelgelil

et al. 2015

Journal of the Egyptian Society of Parasitology

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“…Said et al (2011) in Cairo University Children's Hospital reported that of sixty cases, 16 (26.7%) were positive for toxoplasmosis by PCR, of which 15 (25%) had low avidity of IgG antibodies (positive). Saleh et al (2014) in Alexandria General Hospital among childbearing age females reported that 22.2% pregnant women and 20% of non-pregnant ones had IHAT antibodies against T. gondii. On the other hand, anti-Toxoplasma antibodies were reported the edible animals (Rifaat et al, 1977), camels (Hilali et al, 1998), rabbits (Harfoush and Tahoon, 2010) as well as equines (Haridy et al, 2009), donkeys' milk (Haridy et al, 2010), birds (Rifaat et al, 1969), as well as domestic and wild rodents (Morsy et al, 1987).…”

Section: Methodsmentioning

confidence: 99%

Modified Dot Elisa for Diagnosis of Zoonotic Toxoplasmosis in Man and Sheep with Clinical Suggestive Manifestations

El-Bahy¹,

Geneidy²,

Mahrous³

2017

JESP

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Fractionation of Toxoplasma gondii RH strain tachyzoites (TTAg) using SDS-PAGE and identification of its specific bands versus control sera using EITB revealed five group of specific fractions corresponding to molecular weight (MW) level of 32-34 kda, 46kda, 53 kda, 78 kda and 100 kda. Fractions of 32-34 kda were the most sensitive and specific one able to capturing very low level of anti-Toxoplasma Ab in the sera and did not cross react with anti-parasites antibodies (Ab) as hydatedosis, S. mansoni, HCV, F. gigantica and Cysticercus ovies as well as control noninfected sera. Thick transverse gel strip containing the fraction of 32-34kda were cut out, its contents were eluted and concentrated, Dotting of 2μl from this fraction on 4mm diameter nitrocellulose (NC) discs for development a modified dot-ELISA technique were evaluated in diagnosis of toxoplasmosis in group of suspected women and sheep. This technique succeeded in diagnosis of the specific anti-toxoplasma Ab in sera of seven suspected sheep, all of them aborted after this. Investigation of some haematological and serum chemical parameters demonstrated significant (p≤0.05) deviations in the values of WBCs, AST, Urea and Cholesterol, which considered to be important in supporting early diagnoses of infection in suspected cases. Adaptation of modified Dot-ELISA technique described in this work perform an easily field applied mass screening technique depends on using a minute amount of serum without special equipment. Dot-ELISA by this way gathering the benefits of accuracy of EITB and overcome the cross reaction phenomenon characteristic to ELISA. This improved capability of early diagnosis and control strategy of toxoplasmosis in man and animals.

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Screening of Toxoplasma Gondii Infection among Childbearing Age Females and Assessment of Nurses' Role in Prevention and Control of Toxoplasmosis

Cited by 25 publications

References 0 publications

Nurses´ Expectations toward Toxoplasmosis during Pregnancy

Nurses´ Expectations toward Toxoplasmosis during Pregnancy

Parasites Transmitted to Human by Ingestion of Different Types of Meat, El-Minia City, El-Minia Governorate, Egypt

Modified Dot Elisa for Diagnosis of Zoonotic Toxoplasmosis in Man and Sheep with Clinical Suggestive Manifestations

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