Screening of Urfa pepper landraces for resistance to Meloidogyne incognita

NAS, Yahya; Özalp, Tevfik; Devran, Zübeyir

doi:10.1007/s41348-022-00673-w

Cited by 4 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A total of 1000 J2s were inoculated into two holes (0.5 ml of water per hole) near the stem of each plant. The procedure was performed according to former studies (Öcal et al, 2018;Nas et al, 2023). After seven days, the plants were transferred to a growth chamber at 25°C ± 0.5 with a 16:8 hours photoperiod (light: dark) and 65 ± 5% relative humidity until the end of the experiment.…”

Section: Testing Plant Lines With Nematodementioning

confidence: 99%

Mapping of the gene in tomato conferring resistance to root-knot nematodes at high soil temperature

Devran,

Özalp,

Studholme

et al. 2023

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Root-knot nematodes (RKNs, Meloidogyne spp.) can cause severe yield losses in tomatoes. The Mi-1.2 gene in tomato confers resistance to the Meloidogyne species M. incognita, M. arenaria and M. javanica, which are prevalent in tomato growing areas. However, this resistance breaks down at high soil temperatures (>28°C). Therefore, it is imperative that new resistance sources are identified and incorporated into commercial breeding programmes. We identified a tomato line, MT12, that does not have Mi-1.2 but provides resistance to M. incognita at 32°C soil temperature. An F2 mapping population was generated by crossing the resistant line with a susceptible line, MT17; the segregation ratio showed that the resistance is conferred by a single dominant gene, designated RRKN1 (Resistance to Root-Knot Nematode 1). The RRKN1 gene was mapped using 111 Kompetitive Allele Specific PCR (KASP) markers and characterized. Linkage analysis showed that RRKN1 is located on chromosome 6 and flanking markers placed the locus within a 270 kb interval. These newly developed markers can help pyramiding R-genes and generating new tomato varieties resistant to RKNs at high soil temperatures.

show abstract

Section: Testing Plant Lines With Nematodementioning

confidence: 99%

Mapping of the gene in tomato conferring resistance to root-knot nematodes at high soil temperature

Devran,

Özalp,

Studholme

et al. 2023

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

SCAR markers linked to Phytophthora capsici and Meloidogyne incognita resistance in Piper sp.

Truong,

Ho,

Rasphone

et al. 2024

Plant Pathology

View full text Add to dashboard Cite

Black pepper (Piper nigrum) cultivation suffers from pathogenic Phytophthora capsici and Meloidogyne incognita, causing tremendous yield loss. To solve this problem, genetically sustainable disease‐resistant accessions need to be selected and bred. However, the lack of genomic DNA data for Piper sp. could be a major obstacle. In this study, we created R‐pools and S‐pools from resistant and susceptible accessions in bulked segregant analysis (BSA), respectively, selected DNA fragments associated with resistance or susceptibility to P. capsici and M. incognita from RAPD markers and then converted them into SCAR markers. This is the first publication showing the potential of molecular markers, specifically the SCAR marker, to select Piper sp. accessions resistant to both P. capsici and M. incognita. Based on BSA, four RAPD primers were selected: UBC#377, UBC#359, UBC#360 and UBC#408. Five resistance and susceptibility‐specific fragments were isolated, cloned and sequenced to synthesize 16 SCAR primers. We converted the UBC#377 marker into a 46‐377F2R1 marker and the UBC#360 marker into a 30‐360F1R2 marker. While the 46‐377F2R1 marker has the potential to identify P. capsici‐resistant accessions with a 350 bp resistance‐associated band, the 30‐360F1R2 marker showed the potential to find M. incognita‐resistant accessions with a 230 bp resistance‐linked band.

show abstract

Comparison of effectiveness of molecular markers linked to Me1 and N genes in pepper (Capsicum annuum L.) (Solanales: Solanaceae)

Uysal,

Devran

2024

TED

View full text Add to dashboard Cite

Pepper (Capsicum annuum L.) (Solanales: Solanaceae) is one of the most important agricultural products consumed in the world. Root-knot nematodes (RKNs (Meloidogyne spp.)) are major pests that occur dramatically damage on pepper. However, the management of RKNs has some difficulties and one of the most effective methods is using resistant cultivars in infested areas. In this study, the efficiency of molecular markers linked to Me1 and N genes was investigated. The study was conducted in laboratory and under controlled conditions at Akdeniz University Faculty of Agriculture, Department of Plant Protection Nematology Laboratory in 2022. Pepper genotypes belonging to two main varieties (Charleston pepper and Bell pepper) were tested against S6 isolate of Meloidogyne incognita (Kofoid & White, 1919) Chitwood 1949 (Tylenchida: Heteroderidae), and screened with molecular markers. As a result, molecular markers linked to two genes gave compatible results with pathologic tests. These markers can be successfully used for marker assisted selection in pepper genotypes.

show abstract

Screening of Urfa pepper landraces for resistance to Meloidogyne incognita

Cited by 4 publications

References 27 publications

Mapping of the gene in tomato conferring resistance to root-knot nematodes at high soil temperature

Mapping of the gene in tomato conferring resistance to root-knot nematodes at high soil temperature

SCAR markers linked to Phytophthora capsici and Meloidogyne incognita resistance in Piper sp.

Comparison of effectiveness of molecular markers linked to Me1 and N genes in pepper (Capsicum annuum L.) (Solanales: Solanaceae)

Contact Info

Product

Resources

About