Screening, Optimization and Extraction of Polyhydroxyalkanoates (PHAs) from Bacillus thuringienesis

Desouky, Said E.; El-Shiekh, H.; El-Abd, Mostafa A; Shehab, Amr M.

doi:10.9734/jabb/2014/12286

Cited by 16 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The agar plates were observed under UV light to identify fluorescence of each colony. Eight out of 11 colonies, which fluoresce under UV light, are positive colonies producing bioplastics [15]. However, only one isolate gave maximum fluorescence and was selected to produce PHB; this was bacterial strain TN-TH 01 shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of Poly-(R)-3-hydroxybutyrate Produced by Bacillus thuringiensis TH-01

Rizki

Sabiqoh

Ratnaningsih

et al. 2021

KEM

View full text Add to dashboard Cite

Poly-(R)-3-hydroxybutyrate (PHB) is a biopolymer that can be synthesized by several microorganisms such as bacteria, yeast, and fungi as secondary metabolites. PHB is produced by bacteria in a medium containing a limited amount of key nutrients such as nitrogen, phosphorus, and magnesium but rich in carbon sources. PHB is a biodegradable plastic that has many applications in the medical and industrial fields. This study aimed to isolate and characterize a biopolymer produced by a bacterium strain isolated from a termite nest in India that was identified by 16S rRNA method as Bacillus thuringiensis TH-01. The biopolymer was produced by growing the bacteria in a high medium overnight at 37 °C in a shaking incubator at 150 rpm, and the resulting biopolymer was extracted with a mixture of chloroform–NaOCl (1:1). The efficiency of biopolymer production was about 10.545% ± 26.125%. Fourier transform infrared analysis gave prominent absorption peaks at 3400 cm−1 (stretching of O–H), 2900 cm−1 (stretching of C–H), 1700 cm−1 (stretching of C=O), 1280 cm−1 (symmetric deformation of C–H), and 1050 cm−1 (stretching of C−O), confirming that the biopolymer is PHB. The thermal stability of PHB granules as was determined by thermogravimetry analysis (TGA) and differential scanning calorimetry (DSC) showed that the decomposition temperature and of the polymer were 271.6–310.0 °C and 7.48 J/g respectively, and its crystallinity was about 5.12%.

show abstract

Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of Poly-(R)-3-hydroxybutyrate Produced by Bacillus thuringiensis TH-01

Rizki

Sabiqoh

Ratnaningsih

et al. 2021

KEM

View full text Add to dashboard Cite

show abstract

“…Os PHAs são biopolímeros biodegradáveis que podem ser obtidos de bactérias e outros microrganismos (DESOUKY et al, 2014). Bactérias acumulam intracelularmente P(3HB) na forma de corpos lipofílicos como reserva energética quando expostas a meio com restrições de nutrientes (sobretudo nitrogênio), porém quando há grande disponibilidade de carbono (SUDESH et al, 2000).…”

Section: Introductionunclassified

Aproveitamento de torta do tabaco gerado na produção de biodiesel para a obtenção de bioplástico

Riça

Moraes

Souza

et al. 2016

RJP

View full text Add to dashboard Cite

O poli 3-hidroxibutirato – P(3HB) – é o principal polímero da classe dos poli hidroxialcanoatos (PHAs), polímeros biodegradáveis sintetizados por microrganismos a partir de diversos substratos. O objetivo desse trabalho foi a produção de P(3HB) a partir do resíduo da extração do óleo da semente de tabaco (Nicotiana tabacum L.) como substrato alternativo e de baixo custo para triagem de microrganismos produtores de P(3HB). O resíduo foi avaliado quanto a sua composição e beneficiado através de hidrólise. O hidrolisado foi utilizado em ensaio de fermentação com cepas bacterianas isoladas da Estação de Tratamento de Efluentes (ETE) da Universidade de Santa Cruz do Sul (UNISC). Para visualização de inclusões lipofílicas nas células, indicadoras de acúmulo de P(3HB), utilizou-se coloração de Sudan Black. A extração do polímero do interior das células foi realizada utilizando-se clorofórmio e hipoclorito de sódio e o produto extraído foi analisado em espectrofotômetro. De 25 isolados, a cepa E10 foi a que apresentou melhor resultado frente à coloração de Sudan Black. A análise do produto obtido sugere que a E10 produziu PHAs utilizando o resíduo de tabaco, demonstrando seu potencial como substrato de baixo custo para crescimento microbiano e obtenção de biopolímeros.

show abstract

Green synthesis of polyhydroxyalkanoate polymer by Bacillus iocasae

Ammar

El-Sheshtawy

El-Shatoury

et al. 2021

Polymer International

View full text Add to dashboard Cite

Polyhydroxyalkanoates (PHAs) are bio-polyesters synthesized by various microbial species as a potential replacement for conventional fossil-fuel-based plastic. The aim of the present work was to produce PHAs by a bacterial isolate recovered from activated sewage sludge. The bacterial isolate was identified using 16S rRNA as Bacillus iocasae MMAS4. A Plackett-Burman (PB) design was used to estimate the PHA percentage content using the bacterial strain per 100 mL modified Luria-Bertani broth medium. Factors evidencing P values of less than 0.05 were considered to have significant effects on the PHA percentage. The production of PHAs was improved by adding stress factors such as ethanol, H 2 O 2 and a 1:1 mixture of both into the culture medium. B. iocasae is considered a novel PHA producer yielding 1.5 g L −1 PHAs. The produced PHAs were extracted and characterized. The functional groups of the extracted PHA granules were characterized as P(3HB-co-3-HV) copolyester (poly-3-hydroxybutyrate-poly-3-hydroxyvalerate) by Fourier transform infrared spectroscopy and 1 H NMR analyses. The results indicated the possible use of B. iocasae for the production of PHAs on media containing molasses, an inexpensive substrate as an alternative to the conventionally chemically synthesized polymers.

show abstract

Screening, Optimization and Extraction of Polyhydroxyalkanoates (PHAs) from Bacillus thuringienesis

Cited by 16 publications

References 25 publications

Isolation and Characterization of Poly-(<i>R</i>)-3-hydroxybutyrate Produced by <i>Bacillus thuringiensis</i> TH-01

Isolation and Characterization of Poly-(<i>R</i>)-3-hydroxybutyrate Produced by <i>Bacillus thuringiensis</i> TH-01

Aproveitamento de torta do tabaco gerado na produção de biodiesel para a obtenção de bioplástico

Green synthesis of polyhydroxyalkanoate polymer by Bacillus iocasae

Contact Info

Product

Resources

About