Screening orchardgrass (<i><scp>D</scp>actylis glomerata </i><scp>L</scp>.) germplasm for high rust resistance and high summer survival rate in a subtropical climate

Yan, Haidong; Zeng, Bing; Zhang, Xinquan; Cheng, Liang; Miller, Stephen J.; Huang, Linkai

doi:10.1111/grs.12030

Cited by 5 publications

(6 citation statements)

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“…A total of 33 orchardgrass accessions, with high level of resistance of disease (HR) and high susceptibility of disease (HS), as evaluated by Yan et al [ 6 ], were chosen for the experiment, including 17 with HR and 16 with HS in 2011 or 2012 (Table 3 ). The germplasm of orchardgrass used in this study consisted of 33 accessions that originated from Asia, Africa, Europe, and Oceania.…”

Section: Methodsmentioning

confidence: 99%

“…Miller and Carlson [ 5 ] evaluated rust resistance in orchardgrass based on both phenotypic performance and a polycross progeny test through phenotypic selection. Yan et al [ 6 ] investigated 242 germplasm resources of orchardgrass for 2 years, finding that 13 accessions exhibited high resistance to rust disease. However, the phenotype of an organism can be influenced by both its genotype and its environment.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide association study of rust traits in orchardgrass using SLAF-seq technology

et al. 2017

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BackgroundWhile orchardgrass (Dactylis glomerata L.) is a well-known perennial forage species, rust diseases cause serious reductions in the yield and quality of orchardgrass; however, genetic mechanisms of rust resistance are not well understood in orchardgrass.ResultsIn this study, a genome-wide association study (GWAS) was performed using specific-locus amplified fragment sequencing (SLAF-seq) technology in orchardgrass. A total of 2,334,889 SLAF tags were generated to produce 2,309,777 SNPs. ADMIXTURE analysis revealed unstructured subpopulations for 33 accessions, indicating that this orchardgrass population could be used for association analysis. Linkage disequilibrium (LD) analysis revealed an average r2 of 0.4 across all SNP pairs, indicating a high extent of LD in these samples. Through GWAS, a total of 4,604 SNPs were found to be significantly (P < 0.01) associated with the rust trait. The bulk analysis discovered a number of 5,211 SNPs related to rust trait. Two candidate genes, including cytochrome P450, and prolamin were implicated in disease resistance through prediction of functional genes surrounding each high-quality SNP (P < 0.01) associated with rust traits based on GWAS analysis and bulk analysisConclusionsThe large number of SNPs associated with rust traits and these two candidate genes may provide the basis for further research on rust resistance mechanisms and marker-assisted selection (MAS) for rust-resistant lineages.Electronic supplementary materialThe online version of this article (doi:10.1186/s41065-017-0027-3) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-wide association study of rust traits in orchardgrass using SLAF-seq technology

et al. 2017

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“…However, each of these studies is more than 20 years old, and the majority of the sponsoring breeding programs no longer exist. The focus of more recent germplasm evaluation was Mediterranean, or winter active, accessions with little potential for most North American locations (Pecetti et al, 2009; Shaimi et al, 2009), efforts for Asian production in Japan and China (Sanada et al, 2007; Yan et al, 2013), and nonflowering orchardgrass (Casler et al, 2013; 2014). Renewed efforts in North America focus on germplasm evaluation for irrigated conditions of the Upper West and Intermountain regions (Bushman et al, 2012; Robins et al, 2012a; 2012b).…”

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Genetic Variation for Dry Matter Yield, Forage Quality, and Seed Traits Among the Half‐Sib Progeny of Nine Orchardgrass Germplasm Populations

et al. 2015

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A potential strategy to address the lack of success of North American orchardgrass (Dactylis glomerata L.) breeding programs to increase forage yield and other agronomic traits is the incorporation of novel sources of germplasm. In an attempt to identify novel orchardgrass germplasm sources with agronomic potential, the study described herein characterized 162 half‐sib families (HSFs) from six orchardgrass germplasm populations and three orchardgrass cultivars. Study conditions were Millville, UT, and Rexburg, ID, field sites with data collection from 2008 to 2010. The genotype × location interaction variance differed from zero for dry matter yield, crude protein, in vitro true digestibility (IVTD), neutral detergent fiber, and seed weight. Within location broad‐sense heritability differed for all traits at both locations (0.36 ±0.10 to 0.77 ±0.03) except IVTD at Rexburg. For each trait there were HSFs that possessed values similar to or better than the included check cultivars (CCs). Additionally, in several instances the mean phenotype of the HSFs from a specific family was better than the phenotype of the corresponding parental population and/or the mean phenotype of the CCs. Overall, for each trait there existed sufficient genetic variation to develop an elite orchardgrass breeding program for irrigated conditions.

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“…A total of 75 orchardgrass accessions, with resistance of disease (R), high level of resistance of disease (HR), susceptibility of disease (S), and high susceptibility of disease (HS), as evaluated by Yan et al . [8], were chosen as experimental materials, and 31 out of the 75 accessions were scored as having HR or R, while the others (44 out of 75) were scored as having S or HS in 2011 or 2012 (Table S5). The HR, R, S, and HS groups were assigned to 6, 5, 2, 1, respectively, to be the phenotypic value for next association analysis (Table S5).…”

Section: Methodsmentioning

confidence: 99%

“…Yan et al . [8] also screened 13 accessions with high disease resistance for a period of two years by evaluating the proportion of rust sorus on the leaves. All these studies depended on observing morphological characters; however, phenotypic traits are highly impacted by environmental conditions [9].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Association of EST-SSR and SCoT Markers with Rust Traits in Orchardgrass (Dactylis glomerata L.)

Yan

Zeng

et al. 2016

Molecules

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Orchardgrass (Dactylis glomerata L.), is a well-known perennial forage species; however, rust diseases have caused a noticeable reduction in the quality and production of orchardgrass. In this study, genetic diversity was assessed and the marker-trait associations for rust were examined using 18 EST-SSR and 21 SCoT markers in 75 orchardgrass accessions. A high level of genetic diversity was detected in orchardgrass with an average genetic diversity index of 0.369. For the EST-SSR and SCoT markers, 164 and 289 total bands were obtained, of which 148 (90.24%) and 272 (94.12%) were polymorphic, respectively. Results from an AMOVA analysis showed that more genetic variance existed within populations (87.57%) than among populations (12.43%). Using a parameter marker index, the efficiencies of the EST-SSR and SCoT markers were compared to show that SCoTs have higher marker efficiency (8.07) than EST-SSRs (4.82). The results of a UPGMA cluster analysis and a STRUCTURE analysis were both correlated with the geographic distribution of the orchardgrass accessions. Linkage disequilibrium analysis revealed an average r 2 of 0.1627 across all band pairs, indicating a high extent of linkage disequilibrium in the material. An association analysis between the rust trait and 410 bands from the EST-SSR and SCoT markers using TASSEL software revealed 20 band panels were associated with the rust trait in both 2011 and 2012. The 20 bands obtained from association analysis could be used in breeding programs for lineage selection to prevent great losses of orchardgrass caused by rust, and provide valuable information for further association mapping using this collection of orchardgrass.

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Screening orchardgrass (Dactylis glomerata L.) germplasm for high rust resistance and high summer survival rate in a subtropical climate

Cited by 5 publications

References 15 publications

Genome-wide association study of rust traits in orchardgrass using SLAF-seq technology

Genome-wide association study of rust traits in orchardgrass using SLAF-seq technology

Genetic Variation for Dry Matter Yield, Forage Quality, and Seed Traits Among the Half‐Sib Progeny of Nine Orchardgrass Germplasm Populations

Genetic Diversity and Association of EST-SSR and SCoT Markers with Rust Traits in Orchardgrass (Dactylis glomerata L.)

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