Screening Potential Antioxidant and Antibacterial Activities of Protein Hydrolysates Derived From Germinated Lablab Bean, Pigeon Pea and Kidney Bean

Ratnayani, Ketut; Panjaitan, Indriani Wisnu Susanto; Puspawati, Ni Made

doi:10.24843/jhsm.2017.v01.i01.p07

Cited by 4 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was followed by other formulations, which had varying levels of pigeon pea. Pigeon pea hydrolysates have been reported to demonstrate antioxidant activities 38 . The presence of saturated double bonds and benzene rings joined by a planar unsaturated C2 structure in the structure of bioactive compounds enhances this antioxidant effect 39 .…”

Section: Antioxidant Activity Of Mixtures Of Hydrolysed Germinated So...mentioning

confidence: 99%

Antioxidant and cytotoxic activities of protein hydrolysates from shrimp shell wastes, germinated soybean and pigeon pea flour blends: A mixture response surface methodology approach

Uro-Chukwu,

Okoli,

Okpala

et al. 2024

J. Drug Delivery Ther.

View full text Add to dashboard Cite

Cancer has high prevalence rate and mortality with conventional chemotherapy and other management protocols being both expensive and inaccessible especially in low/medium income countries (LMIC). Sourcing alternative cheaper and easily accessible treatment from blends of antioxidants sources can reduce the burden of cancer on patients. This work therefore seeks to produce a blend from the protein hydrolysates of shrimp shell waste, germinated soybean and germinated pigeon pea which not only has high antioxidant activity but also can inhibit cervical cancer cell proliferation. In vitro antioxidant and cytotoxic activities of the mixtures of germinated pigeon pea, germinated soybean, and shrimp shell waste hydrolysates were evaluated using the mixture response surface methodology (MRSM). Fourteen blends were obtained using the simplex centroid design. Total phenolic content (TPC), 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) and % cytotoxicity of the samples were analysed. Numerical optimization was conducted with the goal of simultaneously maximizing the DPPH scavenging activity and TPC while minimizing % cytotoxicity. The optimized blend consisted of 91.06 % pigeon pea, 8.94 % shrimp shell waste and 0 % soybean. The predicted responses obtained were 74.28 % DPPH scavenging activity, 39.6 GAE mg/dL TPC and 21 % cytotoxicity. The IC50 values for the optimized blend and a standard chemotherapeutic drug were 0.260 mmol/mL and 0.013 mmol / mL respectively. This in vitro study revealed that the pigeon pea - shrimp shell waste blend, as generated by MRSM, was comparable to a standard anticarcinogenic drug with respect to potency. Keywords: pigeon pea, soybean, hydrolysates, shrimp shell waste, cytotoxicity, antioxidants, DPPH

show abstract

Section: Antioxidant Activity Of Mixtures Of Hydrolysed Germinated So...mentioning

confidence: 99%

Antioxidant and cytotoxic activities of protein hydrolysates from shrimp shell wastes, germinated soybean and pigeon pea flour blends: A mixture response surface methodology approach

Uro-Chukwu,

Okoli,

Okpala

et al. 2024

J. Drug Delivery Ther.

View full text Add to dashboard Cite

show abstract

“…The noodle with thymol was unacceptable for consumers with the a barrier against numerous antibiotic molecules and the enzymes of the periplasmic spaces. Ratnayani et al (2017) indicated that protein hydrolysates from beans had no antimicrobial effects on E. coli and S. aureus. This is because protein hydrolysates are mixtures of different types of amino acids and are of different sizes.…”

Section: Sensory Properties Of Cooked Noodlesmentioning

confidence: 99%

Antimicrobial activity of a crude peptide extract from lablab bean (Dolichos lablab) for semi-dried rice noodles shelf-life

Bai-Ngew

Chuensun

Wangtueai

et al. 2021

qas

View full text Add to dashboard Cite

This study provides an application of new, natural source of crude peptide extract from Lablab bean (CPL). Use of additive such as benzoate in the rice noodle industry is a common practice and has several bene?cial effects on quality and shelf-life. However, the shelf-life of semi-dried rice noodles can be extended by crude peptide extract with acceptable quality when compared to other additives. This research aimed to extract CPL and determine its effect on the growth of microorganisms. The use of the CPL to extend the shelf-life of semi-dried rice noo-dles was compared with other natural extracts (chitosan and thymol) and benzoic acid. The CPL samples were extracted using 5% pepsin and incubated for up to 24 h. CPL at 200 mg/mL could be used as the growth inhibitor for Bacillus cereus but not for Staphylococcus aureus and Escherichia coli. It was found that noodles with CPL had the highest cooking loss (4.69) and lowest tensile strength (22.6 g.force). Overall-liking scores showed slightly liked (6.0 out of 9) and 68% of the consumers accepted the CPL-treated noodles. Shelf-life testing showed that CPL could extend the shelf-life of the noodles for 3 days than the control (1 day). Using 200 mg/mL of CPL could extend the shelf-life more than 3 times when compared to the control noodle (no additive). The CPL was nearly as effective as benzoic acid that could be kept for 3 days. Hedonic score in overall-liking showed a slightly like (6.2) for the noodles with CPL. This study suggests the application of adding CPL could be used as new natural additive and seems to be promising to apply in many food products such as pasta or sausages.

show abstract

Learning motivation, learning achievement, and attitude towards the use of cloud service

Kurniawan,

Fitriani,

Loeneto

2023

AIP Conference Proceedings

View full text Add to dashboard Cite

Screening Potential Antioxidant and Antibacterial Activities of Protein Hydrolysates Derived From Germinated Lablab Bean, Pigeon Pea and Kidney Bean

Cited by 4 publications

References 5 publications

Antioxidant and cytotoxic activities of protein hydrolysates from shrimp shell wastes, germinated soybean and pigeon pea flour blends: A mixture response surface methodology approach

Antioxidant and cytotoxic activities of protein hydrolysates from shrimp shell wastes, germinated soybean and pigeon pea flour blends: A mixture response surface methodology approach

Antimicrobial activity of a crude peptide extract from lablab bean (Dolichos lablab) for semi-dried rice noodles shelf-life

Learning motivation, learning achievement, and attitude towards the use of cloud service

Contact Info

Product

Resources

About