Screening Potential of Solid-Phase Extraction Room Temperature Phosphorimetry for the Analysis of Polychlorinated Dibenzofurans in Water Samples

Abstract

Solid-phase extraction room-temperature phosphorimetry is evaluated for screening polychlorinated dibenzofurans in water samples. The organic pollutants are extracted from water samples on octadecyl bonded silica membranes. Water extraction is rapidly performed on a 13 mm disk by using a 100 mL syringe and a stainless steel filter holder. Room-temperature phosphorimetry is directly performed on the extraction membrane, which results in analysis times of approximately 10 min per sample. Limits of detection at the sub-parts-per-billion (ng‚mL -1 ) are obtained with 100 mL of water sample. The selectivity of the technique is appropriate for total polychlorinated dibenzofurans analysis in the presence of concomitants such as pesticides, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, and polychlorinated dibenzo-p-dioxins. At the present stage, this methodology can obviate costly chromatographic analysis of uncontaminated water samples. Since it is a nondestructive technique, it could be used in tandem with chromatographic analysis for specific compound identification of positive samples.

Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

“…After drying for 5 min, the tabs were examined via front surface room-temperature fluorescence spectroscopy to reach limits of detection (LODs) at the parts-per-billion (ng·mL − 1 ) level [5,13]. Later reports extended the use of SPE membranes to the room temperature phosphorimetry (RTP) analysis of PAHs [6,[14][15][16][17], polychlorinated biphenyls [15,17] and polychlorinated dibenzofurans [17]. Bulky glassware and vacuum pumps, common to classic SPE lab procedures, were replaced with a syringe kit well-suited for manual extraction under field conditions.…”

Detection of 1-hydroxypyrene in urine by direct fluorometric analysis on a solid sorbing phase. Validation and application of the method to biological monitoring of PAH-exposed persons

“…The present work represents a new development and application of analytical methods based on the selective adsorption of hydrophobic pollutants on a solid sorbent associated with spectroscopic detection directly applied on the solid substrate [19,20,21,22,23,24,25,26,27,28,29]. The evaluation of the potential of this approach for the analysis of 1-OHPy in human urine demonstrates that the method we propose is very sensitive and can be applied to the biological monitoring of people differently exposed or not to PAH.…”

“…Previous works [19,20,21,22,23,24,25,26,27,28,29] have shown that, using appropriate solid sorbent for extraction/concentration, it is possible to avoid the elution required in the solid phase and chromatographic steps by performing in situ spectroscopic detection of benzenic compounds [20], PAH [21,22,23,24,25,26,27] or polychlorobiphenyls [28,29] in aqueous solution directly on the solid phase. Recently, this approach has been proposed for the detection of 1-OHPy in human urine [24].…”

Phosphorescence Properties of p-Aminobenzoic Acid Immobilized on a Nylon Membrane

“…On the other hand, the biexponential decays obtained in the more complex systems are likely to result from their heterogeneous composition, which expose PABA molecules to different environments. 36 Interferences. With the aim of determining possible interferences with PABA phosphorescence by molecules bearing NH groups, compounds usually found in the same pharmaceuticals that contain PABA (with or without native phosphorescence) were investigated, namely nicotinamide, folic acid, pyridoxine (vitamin B 6 ), and cyanocobalamin (vitamin B 12 ).…”