Screening study for antibacterial activity from Marine and freshwater microalgae

Corona, Enrique González; Fernandez-Acero, J; Bartual, Ana

doi:10.22376/ijpbs.2017.8.1.p189-194

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…At 37°C, the plates were incubated for 24 h. Around the filter disks, antibacterial extracts produced distinct, transparent, and circular zones of inhibition. This positive activity was calculated by measuring the growth inhibition zone (mm) surrounding the disks after 24 h (Corona et al., 2017 ). A disk loaded with only ethanol was prepared as a negative control.…”

Section: Methodsmentioning

confidence: 99%

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Microwave‐assisted extraction optimization of sesquiterpene lactones from Inula helenium roots: A sustainable approach to reduce energy consumption and carbon footprint

Özcan,

Dikmen,

Özcan

et al. 2023

Food Science & Nutrition

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Inula helenium roots are consumed as natural flavor components and raw or cooked as food, and their extracts are rich in sesquiterpene lactones such as alantolactone (AL) and isoalantolactone (IAL), which have recently attracted great attention due to their pharmacological properties. The industrial utilization of these compounds requires the development of green, efficient, cost‐effective, and sustainable extraction protocols. Therefore, this study focused on the optimization of microwave‐assisted extraction (MAE) process variables using Face‐Centered Central Composite Design (FC‐CCD). Then, maceration was applied as a conventional technique, and these techniques were compared in terms of extraction efficiency, morphological changes, antimicrobial activities, carbon emissions, and energy consumption. As a result, optimal MAE conditions, i.e., EtOH: water ratio (X1) = 100:0, liquid/sample ratio (X2) = 30:1 mL/g, microwave power (X3) = 300 W, and irradiation time (X4) = 5 min, were obtained with AL and IAL yields of 54.99 ± 0.11 (mg/g) and 48.40 ± 0.19 (mg/g), respectively. The extract obtained by MAE had similar or better activity than positive controls in most cases and formed the largest inhibition zones against E. coli (29.5 ± 0.71 mm) and A. niger (34.75 ± 1.06 mm). Morphological changes of I. helenium roots after extraction were observed by scanning electron microscopy. Additionally, MAE was 43.4 times faster than maceration, resulting in 228.6 times less energy consumption and carbon emissions. Based on these findings, it is recommended to use MAE as an industrial green technique for the extraction of sesquiterpene lactones with potential applications in nutraceuticals and food products in terms of sustainable economy and environmental protection.

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Section: Methodsmentioning

confidence: 99%

“…By using the disk diffusion method, the qualitative antibacterial zone (mm) surrounding the disks after 24 h (Corona et al, 2017).…”

Section: Antibacterial Activitymentioning

confidence: 99%

Microwave‐assisted extraction optimization of sesquiterpene lactones from Inula helenium roots: A sustainable approach to reduce energy consumption and carbon footprint

Özcan,

Dikmen,

Özcan

et al. 2023

Food Science & Nutrition

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The importance of harvesting time on the screening of Chlamydomonas spp. extracts for antibacterial activity

N.A.¹,

Lannér²,

Scott³

2020

Algologia

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Изучен ростовой цикл 5 штаммов Chlamydomonas Ehrenberg (Сhlorophyta), выделенных из разных водоемов в Онтарио (Канада). Ранее было показано, что они обладают ингибирующей активностью в отношении Staphylococcus aureus. Зеленые микроводоросли известны своей способностью продуцировать метаболиты с антибактериальной активностью, но данных о том, продуцируют ли они антибактериальные соединения на протяжении всех фаз роста и в какой момент ростового цикла они продуцируют их максимальное количество, пока очень мало. Для массового скрининга таких штаммов важно знать, в какой момент ростового цикла клетки начинают вырабатывать биоактивные соединения и когда они достигают максимальных уровней их производства. Аксенические культуры микроводорослей выращивали в инкубаторе. Скорость роста штаммов определяли методом спектрофотометрии при 550 нм каждые 7 дней. Отбор биомассы для экстрагирования также проводили каждые 7 сут, начиная с ранней экспоненциальной фазы и заканчивая на 49-е сут, когда культуры находились на ранней стационарной фазе роста. Результаты показали,

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Screening study for antibacterial activity from Marine and freshwater microalgae

Cited by 2 publications

References 21 publications

Microwave‐assisted extraction optimization of sesquiterpene lactones from Inula helenium roots: A sustainable approach to reduce energy consumption and carbon footprint

Microwave‐assisted extraction optimization of sesquiterpene lactones from Inula helenium roots: A sustainable approach to reduce energy consumption and carbon footprint

The importance of harvesting time on the screening of Chlamydomonas spp. extracts for antibacterial activity

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