Screening the immunotoxicity of different food preservative agents on the model organism <i>Galleria mellonella</i> L. (Lepidoptera: Pyralidae) larvae

Erbaş, Emine Duman; Gwokyalya, Rehemah; Altuntaş, Hülya; Kutrup, Bilal

doi:10.1080/01480545.2022.2091589

Cited by 3 publications

(2 citation statements)

References 68 publications

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“…G. mellonella is a robust model organism that has gained popularity in recent decades. This model organism has been used for a wide range of assays including virulence assays (bacterial and fungal), immunotoxicity testing (concerning the adaptive immune system), drug toxicity assays, testing the effects of ultraviolet radiation, probiotics, and bacteriophage treatment efficacy (Cunha et al ., 2023, Stempinski, Smith and Casadevall 2022, Erbas et al ., 2023, Demirtürk, Uçkan, and Mert, 2023, Sabockyte et al ., 2023, Filippov et al ., 2022, Maslova et al ., 2023). G. mellonella are affordable, have low ethical considerations associated with their use and have low maintenance and handling requirements which could facilitate faster early-stage pathogen dissemination and prevention strategy testing.…”

Section: Introductionmentioning

confidence: 99%

UsingGalleria mellonellato study pathogen dissemination and anti-infective surface coatings

Maslova,

Staber,

McCarthy

2024

Preprint

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Hospital associated infections and localised hospital outbreaks are a major challenge for infection control teams (ICTs) in hospitals around the world. Advances in artificial intelligence and infection modelling have enabled ICT teams to better predict and trace infection spread. However, it is notoriously difficult to replicate bacterial dissemination or validate prediction models in a laboratory setting due to a lack of effectivein vivomodels. In this work, we sought to developGalleria mellonellaas a model organism to replicate the dissemination of pathogens in a hospital intensive care unit (ICU). By combining this model organism with 3D printed models of a real hospital ICU, we are able to demonstrate that larvae do disseminate the multidrug resistant pathogenAcinetobacter baumanniiwithin this ICU model and that it is possible to use this model to identify infection hotspots. Importantly, this model can also be used for intervention strategy testing as we also show that bacterial dissemination can be significantly mitigated by the introduction of antimicrobial wall surface coatings. This model provides a robust platform for the testing of antimicrobial surface coatings as well as the study of genetic determinants with a role in pathogen dissemination.

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Section: Introductionmentioning

confidence: 99%

UsingGalleria mellonellato study pathogen dissemination and anti-infective surface coatings

Maslova,

Staber,

McCarthy

2024

Preprint

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“…Wax moth Galleria mellonella L. is one of the most commonly used models for toxicological investigations [21][22][23][24], including analysis of the antibacterial action of drugs [25] and in vivo assessment of pathogenicity of various microorganisms [26][27][28]. Wax moth larvae develop toxicosis in the midgut, which is accompanied by changes in the microbiota, digestion activity, and defense systems [11,12].…”

Section: Introductionmentioning

confidence: 99%

A Diet with Amikacin Changes the Bacteriobiome and the Physiological State of Galleria mellonella and Causes Its Resistance to Bacillus thuringiensis

Polenogova,

Klementeva,

Kabilov

et al. 2023

Insects

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Environmental pollution with antibiotics can cause antibiotic resistance in microorganisms, including the intestinal microbiota of various insects. The effects of low-dose aminoglycoside antibiotic (amikacin) on the resident gut microbiota of Galleria mellonella, its digestion, its physiological parameters, and the resistance of this species to bacteria Bacillus thuringiensis were investigated. Here, 16S rDNA analysis revealed that the number of non-dominant Enterococcus mundtii bacteria in the eighteenth generation of the wax moth treated with amikacin was increased 73 fold compared to E. faecalis, the dominant bacteria in the native line of the wax moth. These changes were accompanied by increased activity of acidic protease and glutathione-S-transferase in the midgut tissues of larvae. Ultra-thin section electron microscopy detected no changes in the structure of the midgut tissues. In addition, reduced pupa weight and resistance of larvae to B. thuringiensis were observed in the eighteenth generation of the wax moth reared on a diet with amikacin. We suggest that long-term cultivation of wax moth larvae on an artificial diet with an antibiotic leads to its adaptation due to changes in both the gut microbiota community and the physiological state of the insect organism.

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[Influence of inactivated species of the genus Bacillus Cohn, 1872 on the cellular immunity of Galleria mellonella (Linnaeus, 1758)]

Polenogova,

Zhangissina,

Artemchenko

et al. 2024

euroasentj

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Screening the immunotoxicity of different food preservative agents on the model organism Galleria mellonella L. (Lepidoptera: Pyralidae) larvae

Cited by 3 publications

References 68 publications

UsingGalleria mellonellato study pathogen dissemination and anti-infective surface coatings

UsingGalleria mellonellato study pathogen dissemination and anti-infective surface coatings

A Diet with Amikacin Changes the Bacteriobiome and the Physiological State of Galleria mellonella and Causes Its Resistance to Bacillus thuringiensis

[Influence of inactivated species of the genus Bacillus Cohn, 1872 on the cellular immunity of Galleria mellonella (Linnaeus, 1758)]

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