Screening the keratinolytic activity of dermatophytes in vitro

Wawrzkiewicz, K; Wolski, T.; L̷obarzewski, J.

doi:10.1007/bf00436684

Cited by 85 publications

(63 citation statements)

References 16 publications

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“…The keratin-degrading capability was investigated as described by Wawrzkiewicz et al 53 with some modifications using keratin powder (Sigma) instead of chicken feathers, which was considered as a keratin source. The agar plates of feather meal medium (0.01% Mg 2 SO 4 , 0.14% K 2 HPO 4 , 0.07% KH 2 PO 4 , 0.05% NaCl, 1.0% ground keratin, 1.5% Bacto agar) were inoculated with freshly prepared inocula of tested bacterial and fungal strains.…”

Section: Keratinase Activitymentioning

confidence: 99%

Synthesized zinc peroxide nanoparticles (ZnO<sub>2</sub>-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

Ali¹,

Morsy²,

El-Zawawy³

et al. 2017

IJN

121

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Increasing of multidrug resistance (MDR) remains an intractable challenge for burn patients. Innovative nanomaterials are also in high demand for the development of new antimicrobial biomaterials that inevitably have opened new therapeutic horizons in medical approaches and lead to many efforts for synthesizing new metal oxide nanoparticles (NPs) for better control of the MDR associated with the polymicrobial burn wounds. Recently, it seems that metal oxides can truly be considered as highly efficient inorganic agents with antimicrobial properties. In this study, zinc peroxide NPs (ZnO 2 -NPs) were synthesized using the co-precipitation method. Synthesized ZnO 2 -NPs were characterized by X-ray diffraction, Fourier transformed infrared, transmission electron microscopy, thermogravimetric analysis, differential scanning calorimetry, and ultraviolet-visible spectroscopy. The characterization techniques revealed synthesis of the pure phase of non-agglomerated ZnO 2 -NPs having sizes in the range of 15–25 nm with a transition temperature of 211°C. Antimicrobial activity of ZnO 2 -NPs was determined against MDR Pseudomonas aeruginosa (PA) and Aspergillus niger (AN) strains isolated from burn wound infections. Both strains, PA6 and AN4, were found to be more susceptible strains to ZnO 2 -NPs. In addition, a significant decrease in elastase and keratinase activities was recorded with increased concentrations of ZnO 2 -NPs until 200 µg/mL. ZnO 2 -NPs revealed a significant anti-inflammatory activity against PA6 and AN4 strains as demonstrated by membrane stabilization, albumin denaturation, and proteinase inhibition. Moreover, the results of in vivo histopathology assessment confirmed the potential role of ZnO 2 -NPs in the improvement of skin wound healing in the experimental animal models. Clearly, the synthesized ZnO 2 -NPs have demonstrated a competitive capability as antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory candidates, suggesting that the ZnO 2 -NPs are promising metal oxides that are potentially valued for biomedical applications.

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Section: Keratinase Activitymentioning

confidence: 99%

Synthesized zinc peroxide nanoparticles (ZnO<sub>2</sub>-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

Ali¹,

Morsy²,

El-Zawawy³

et al. 2017

IJN

121

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“…O meio para colagenase foi gelatina 1% (p/v) e ágar 2% (p/v). O crescimento em meio sólido contendo gelatina como fonte de carbono e nitrogênio foi indicativo da produção de colagenase pelos dermatófitos avaliados (Wawrzkiewicz et al 1991).…”

Section: Perfil Enzimáticounclassified

Isolamento e perfil enzimático de cães e gatos com dermatofitose atendidos em hospitais veterinários do Recife, Pernambuco

et al. 2018

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ABSTRACT.-Torres M.E.L.M., Herculano P.N., Lima M.L.F., Soares P.T., Siqueira A.B.S., Souza-Motta C.M., Porto A. Dermatophytes are fungi that can cause superficial infections of the skin, hair and nails in man and animals. The most frequent dermatophyte species isolated from dogs and cats are Microsporum gypseum, most notably Microsporum canis. The crucial role during the infection process is the production of extracellular enzymes essential for the invasion and establishment of the pathogen in the host tissue. The objective of this research was to isolate dermatophytes from dogs and cats and evaluate the enzymatic profile of the isolates obtained. Hair samples and epidermal scales were collected from dogs and cats in veterinary facilities in Recife-PE, and the isolates were identified based on macroscopic and microscopic characteristics. The qualitative analysis of the enzymes urease, protease, lipase, collagenase and phospholipase was evaluated from the isolated dermatophytes. During 10 months, a total of 106 animals, comprising of 99 dogs and seven cats with clinical signs, regardless of sex and race were evaluated. Only eight animals were confirmed with dermatophytosis, mostly dogs (n=7), being six affected by M. canis and one by M. gypseum, the race most affected was Yorkshire (n=3). However, only one cat was confirmed with M. canis. No sex-related predisposition was observed regarding the occurrence of dermatophytosis in dogs and cats evaluated. Isolated dermatophytes showed similar profiles for the enzymes urease, lipase, protease, phospholipase and collagenase, important characteristic for pathogenic infections. The diagnosis of this zoonosis based on microbiological confirmation and a better understanding of the underlying mechanisms is of great importance for the treatment and prevention of fungal diseases in animals.

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“…Twenty fungal isolates were screened by using solid medium method (Wawrzkiewiez et al, 1991). Chicken feather was cut into small fragments, washed extensively with water and detergent and dried in a ventilated oven at 40°C for 72 h to prepare feather powder.…”

Section: Screening Of Fungi For Keratinase Activitymentioning

confidence: 99%

Mycoflora associated with the goats hair and sheep wool in Taif, Saudi Arabia

Awad¹

2017

Afr. J. Microbiol. Res.

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The objective of this study was to evaluate the occurrence of mycoflora in 30 samples of healthy goat's hair and sheep wool collected from different localities in Taif, Saudi Arabia, and the ability of some fungal isolates for keratinase activities. Sixty four species belonging to 28 genera were collected from the two substrates. The wool of sheep was polluted with fungi than goat hairs, and contained high total counts and number of fungal genera and species. Nine species of true dermatophytes isolated belonged to Microsporum (3 species) and Trichophyton (6 species). Several keratinophilic species were isolated of which, Chrysosporium indicum, Chrysosporium keratinophilum and Chrysosporium tropicum were the most prevalent. The commonest saprophytes in order of frequency were members of the genera, Aspergillus, Penicillium, Alternaria and Cochliobolus. In addition, the other genera found included Acremonium, Chaetomum, Cladosporium, Cochliobolus, Fusarium, Mucor, Paecilomyces, Phoma, Rhizopus, Scopulariopsis, Stachybotrys, Trichoderma and others. Five species from 20 tested isolates (Aspergillus niger, C. keratinophilum, C. tropicum, Microsporum gypseum and Trichoderma viride) had high keratinase activity. The results of this study indicate that both goat hair and sheep wool provide a suitable habitat for dermatophytes and other keratinophilic fungi. Most of these fungi play an important role in the degradation of keratin substrates, so that they can help preserve the environment and reduce pollution.

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Screening the keratinolytic activity of dermatophytes in vitro

Cited by 85 publications

References 16 publications

Synthesized zinc peroxide nanoparticles (ZnO<sub>2</sub>-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

Synthesized zinc peroxide nanoparticles (ZnO<sub>2</sub>-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

Isolamento e perfil enzimático de cães e gatos com dermatofitose atendidos em hospitais veterinários do Recife, Pernambuco

Mycoflora associated with the goats hair and sheep wool in Taif, Saudi Arabia

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