Screening Urine Specimen Populations for Normality Using Different Dipsticks: Evaluation of Parameters Influencing Sensitivity and Specificity

Aim: To investigate the positive and negative predictive values for bacteriuria of a rapid chemical dipstick procedure for leukocytes and nitrite, and a microscopic examination for leukocytes and bacteria with a urine culture being the reference in patients with spinal cord lesion (SCL). Methods: A prospective study. Signi®cant bacteriuria was de®ned as 510 5 CFU/mL. The microscopic examination for leukocytes was divided in four dierent cut-o limits for positive results. Material: A total of 256 consecutive early morning urine samples were collected from 143 SCL patients admitted to our in-patient facility. Results: One hundred and twenty-eight urine cultures revealed signi®cant bacteriuria. Eightyseven were infected by only one microorganism, 41 samples contained signi®cant growth of more than one species. A total of 186 microorganisms in signi®cant growth were identi®ed. Conclusion: Three or more leukocytes should be considered as a positive result. The dipstick and microscopy tests are equally valuable, considering the single tests as well as the combinations evaluated.

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“…1,2 It is of importance to evaluate such tests in the population concerned, in this case SCL patients.…”

Section: Introductionmentioning

confidence: 99%

Screening for bacteriuria in patients with spinal cord lesion: Dipstick test, microscopic examination and urine culture

et al. 2000

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“…estimated by several authors (7)(8)(9)(10)(11)(12). They found a good correlation between the "semiquantitative" results of the tested devices and values obtained by quantitative reference methods.…”

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confidence: 99%

On the Performance and Reliability of Mechanized Urine Teststrip Measurement in Comparison with Visual Reading

Marx

Kropf²,

Gressner³

1989

Clinical Chemistry and Laboratory Medicine

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Prescreening of urine specimens by teststrips is a valuable procedure for reducing the work load of the urine analysis iaboratory: positive results for leukocytes, erythrocytes (haemoglobin), protein, and/or nitrite are widely used to select pathological specimens for subsequent microscopic examination. By standardization of the measurement conditions, mechanized teststrip reading is claimed to give more reproducible results than conventional techniques. To assess their ability to improve urine prescreening, especially with regard to the comparability of the results, the practical and analytical performance of three commercially available analysers (Rapimat® II/T from Behringwerke AG, Urotron® RL9 from Boehringer Mannheim GmbH, and Clinitek® 200 from Ames/Bayer Diagnostic) was compared with visual reading. Analytical criteria were assessed using routine urine samples, while reproducibility was tested by repeated analysis of three different commercial control urines (Kova®Trol from Madaus). A mean imprecision between 3% and 11.9% was found for the mechanized dipstick reading which was comparable to that found with visual examination (4.5% with Combur 9 -teststrips, Boehringer Mannheim GmbH). Due to the crude classification of the results, the different analysers äs well äs the visual technique gave quite different distributions for each of the semiquantitative parameters in the same urine samples. Even if statistical analysis was restricted to the frequency of positive results oniy, significant differences (% 2 -test, p < 0.001-0.05) between methods were obtained, but these differences could not be attributed to one method alone. Using microscopic Sediment analysis äs reference, pathological urines were detected with a comparable sensitivity/specificity: Urotron 0.85/0.84, Rapimat 0.91/0.67, Clinitek 0.82/0.81, and duplicate visual reading 0.88/0.67 and 0.91/0.93. Mechanized teststrip reading had no obvious advantage with respect to the time required. We conclude:(i) no improvernent in analytical performance or in speed of analysis could be claimed for mechanized methods in comparison with visual reading;(ii) mechanized teststrip reading might decrease the work load of the urine Iaboratory if integrated into a computerized laboratory System;(iii) mechanized teststrip reading will become analytically advantageous over visual reading if a more refined classification of the results is achieved. troduction^ mechanized teststrip reading have been developed Pre-screening of urine specimens by teststrips is a which are considered to have several advantages in valuable tool for reducing the work load in the urine relation to visual inspection. For example, they are analysis laboratory (l, 2). Positive results for leuko-claimed to give more reproducible results by standcytes, erythrocytes, protein and/or nitrite are widely ardized incubation and measurement conditions. Inused to select pathological samples for microscopic cluding multi-centre evaluation, the reliability of the examination (3 -6). In the last years various ...

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“…Análisis de Orina• Aspecto macroscópico (uroscopia): Aspecto (trasparencia, orinas oscuras y orinas turbias), Color (alimentos, medicamentos, diversas patologías urinarias y extraurinarias). Olor (característico, amoniacal, fecaloideo, pútrido)(90).…”

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Análisis historiográfico de la odontología en Guipúzcoa en el siglo XX

Erce¹

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Screening Urine Specimen Populations for Normality Using Different Dipsticks: Evaluation of Parameters Influencing Sensitivity and Specificity

Cited by 8 publications

References 22 publications

Screening for bacteriuria in patients with spinal cord lesion: Dipstick test, microscopic examination and urine culture

Screening for bacteriuria in patients with spinal cord lesion: Dipstick test, microscopic examination and urine culture

On the Performance and Reliability of Mechanized Urine Teststrip Measurement in Comparison with Visual Reading

Análisis historiográfico de la odontología en Guipúzcoa en el siglo XX

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