2014
DOI: 10.1128/mcb.01346-13
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Screening with a Novel Cell-Based Assay for TAZ Activators Identifies a Compound That Enhances Myogenesis in C2C12 Cells and Facilitates Muscle Repair in a Muscle Injury Model

Abstract: The transcriptional coactivator with a PDZ-binding motif (TAZ) cooperates with various transcriptional factors and plays various roles. Immortalized human mammalian epithelial MCF10A cells form spheres when TAZ is overexpressed and activated. We developed a cell-based assay using sphere formation by TAZ-expressing MCF10A cells as a readout to screen 18,458 chemical compounds for TAZ activators. Fifty compounds were obtained, and 47 were confirmed to activate the TAZ-dependent TEADresponsive reporter activity i… Show more

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Cited by 53 publications
(51 citation statements)
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“…Anti-FLAG, anti-GFP, anti-myosin heavy chain (MHC), antimyogenin, and anti-MyoD antibodies were described previously [6]. The following reagents were obtained from commercial sources; β-guanidinopropionic acid (GPΑ), (Sigma G6878); z-VAD-FMK (Peptide Institute Inc. 3188-v); peroxidase-conjugated antimouse secondary antibody (Cappel); NSV23766 (Santa Cruz sc-204823); and IGF1 (Sigma I8779).…”
Section: Reagents and Antibodiesmentioning
confidence: 99%
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“…Anti-FLAG, anti-GFP, anti-myosin heavy chain (MHC), antimyogenin, and anti-MyoD antibodies were described previously [6]. The following reagents were obtained from commercial sources; β-guanidinopropionic acid (GPΑ), (Sigma G6878); z-VAD-FMK (Peptide Institute Inc. 3188-v); peroxidase-conjugated antimouse secondary antibody (Cappel); NSV23766 (Santa Cruz sc-204823); and IGF1 (Sigma I8779).…”
Section: Reagents and Antibodiesmentioning
confidence: 99%
“…The following reagents were obtained from commercial sources; β-guanidinopropionic acid (GPΑ), (Sigma G6878); z-VAD-FMK (Peptide Institute Inc. 3188-v); peroxidase-conjugated antimouse secondary antibody (Cappel); NSV23766 (Santa Cruz sc-204823); and IGF1 (Sigma I8779). IBS008738 was described previously [6].…”
Section: Reagents and Antibodiesmentioning
confidence: 99%
See 3 more Smart Citations