Scutellarin protects against vascular endothelial dysfunction and prevents atherosclerosis via antioxidation

Mo, Jiao; Yang, Renhua; Li, Fan; Zhang, Xiaochao; He, Bo; Zhang, Yue; Chen, Peng; Zhi-qiang, Shen

doi:10.1016/j.phymed.2018.03.021

Cited by 67 publications

(37 citation statements)

References 30 publications

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“…In our experiments, we observed that the expression of SOD1, a key enzyme involved in the neutralization of superoxide anions ( Fukai and Ushio-Fukai, 2011 ), was significantly decreased in mesenteric vessels from HFD-fed mice, thus suggesting that the endothelial dysfunction elicited by HFD could depend also on the reduced ability of vascular tissues to scavenge superoxide anions. In agreement with our results, recent reports have documented a marked endothelial dysfunction associated with a significant vascular pro-oxidant condition in HFD animals ( Ketonen et al, 2010 ; Heinonen et al, 2014 ; Toral et al, 2015 ), as well as a decreased SOD1 scavenging activity ( Mo et al, 2018 ). Moreover, evidence from clinical studies, showing a correlation of obesity with compromised vascular function, is available also.…”

Section: Discussionsupporting

confidence: 93%

Luteolin Prevents Cardiometabolic Alterations and Vascular Dysfunction in Mice With HFD-Induced Obesity

et al. 2018

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Purpose: Luteolin exerts beneficial effects against obesity-associated comorbidities, although its influence on vascular dysfunction remains undetermined. We examined the effects of luteolin on endothelial dysfunction in a mouse model of diet-induced obesity.Methods: Standard diet (SD) or high-fat diet (HFD)-fed mice were treated daily with luteolin intragastrically. After 8 weeks, body and epididymal fat weight, as well as blood cholesterol, glucose, and triglycerides were evaluated. Endothelium-dependent relaxations of resistance mesenteric vessels was assessed by a concentration-response curve to acetylcholine, repeated upon Nw-nitro-L-arginine methylester (L-NAME) or ascorbic acid infusion to investigate the influence of nitric oxide (NO) availability and reactive oxygen species (ROS) on endothelial function, respectively. Intravascular ROS production and TNF levels were measured by dihydroethidium dye and ELISA, respectively. Endothelial NO synthase (eNOS) and superoxide dismutase 1 (SOD1), as well as microRNA-214-3p expression were examined by Western blot and RT-PCR assays, respectively.Results: HFD animals displayed elevated body weight, epididymal fat weight and metabolic indexes. Endothelium-dependent relaxation was resistant to L-NAME and enhanced by ascorbic acid, which restored also the inhibitory effect of L-NAME, suggesting a ROS-dependent reduction of NO availability in HFD vessels. Moreover, media-lumen ratio, intravascular superoxide anion and TNF levels were increased, while vascular eNOS, SOD1, and microRNA-214-3p expression were decreased. In HFD mice, luteolin counteracted the increase in body and epididymal fat weight, and metabolic alterations. Luteolin restored vascular endothelial NO availability, normalized the media-lumen ratio, decreased ROS and TNF levels, and normalized eNOS, SOD1 and microRNA-214-3p expression.Conclusion: Luteolin prevents systemic metabolic alterations and vascular dysfunction associated with obesity, likely through antioxidant and anti-inflammatory mechanisms.

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Section: Discussionsupporting

confidence: 93%

Luteolin Prevents Cardiometabolic Alterations and Vascular Dysfunction in Mice With HFD-Induced Obesity

et al. 2018

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“…Rats in the control group were fed a normal diet, whereas all other rats were fed a high-fat diet and vitamin D 3 to induce AS (the composition of the rat diet is listed in Table S1) and was in accordance with the literature. 23,24,25 Food was provided adlibitum. After allowing the rats to acclimatize for in the corresponding group.…”

Section: Laboratory Animals and Groupingmentioning

confidence: 99%

<p>Metformin Activates the AMPK-mTOR Pathway by Modulating lncRNA <em>TUG1</em> to Induce Autophagy and Inhibit Atherosclerosis</p>

You¹,

Long²,

Song³

et al. 2020

DDDT

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Background: Metformin has been shown to inhibit the proliferation and migration of vascular wall cells. However, the mechanism through which metformin acts on atherosclerosis (AS) via the long non-coding RNA taurine up-regulated gene 1 (lncRNA TUG1) is still unknown. Thus, this research investigated the effect of metformin and lncRNA TUG1 on AS. Methods: First, qRT-PCR was used to detect the expression of lncRNA TUG1 in patients with coronary heart disease (CHD). Then, the correlation between metformin and TUG1 expression in vitro and their effects on proliferation, migration, and autophagy in vascular wall cells were examined. Furthermore, in vivo experiments were performed to verify the anti-AS effect of metformin and TUG1 to provide a new strategy for the prevention and treatment of AS. Results: qRT-PCR results suggested that lncRNA TUG1 expression was robustly upregulated in patients with CHD. In vitro experiments indicated that after metformin administration, the expression of lncRNA TUG1 decreased in a time-dependent manner. Metformin and TUG1 knockdown via small interfering RNA both inhibited proliferation and migration while promoted autophagy via the AMPK/mTOR pathway in vascular wall cells. In vivo experiments with a rat AS model further demonstrated that metformin and sh-TUG1 could inhibit the progression of AS. Conclusion: Taken together, our data demonstrate that metformin might function to prevent AS by activating the AMPK/mTOR pathway via lncRNA TUG1.

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“…On basis of these results it can be concluded that baicalin is safe and may exert beneficial effect on animals during gestation. [73,35]. It was revealed that Scutellaria has many activities such as cardioprotective [74], vasoprotective [35,75], anti-oxidative, anti-inflammatory, vascular relaxative, anti-platelet and anti-coagulative [76].…”

Section: Influence In Pregnancymentioning

confidence: 99%

“…Recently, Mo et al [73] performed complex studies of scutellarin activity in various models in vitro. Their results showed that various concentrations of scutellarin used to estimation of antioxidative properties (total antioxidant capacity -TAC, 2,2-diphenyl-1picrylhydrazyl -DPPH, superoxide anion, and hydroxyl radical) scavenged all types of radicals in a dose-dependent manner, value of EC 50 in scavenging hydroxyl radical was 37.11 µM.…”

Section: In Vitro and Ex Vivo Studies Related To Hypertension Vasodilmentioning

confidence: 99%

In vitro and in vivo activities of flavonoids – apigenin, baicalin, chrysin, scutellarin – in regulation of hypertension – a review for their possible effects in pregnancy-induced hypertension

et al. 2019

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Summary Flavonoids and their conjugates are the most important group of natural chemical compounds in drug discovery and development. The search for pharmacological activity and new mechanisms of activity of these chemical compounds, which may inhibit mediators of inflammation and influence the structure and function of endothelial cells, can be an interesting pharmacological strategy for the prevention and adjunctive treatments of hypertension, especially induced by pregnancy. Because cardiovascular diseases have multi-factorial pathogenesis these natural chemical compounds with wide spectrum of biological activities are the most interesting source of new drugs. Extracts from one of the most popular plant used in Traditional Chinese Medicine, Scutellaria baicalensis Georgi could be a very interesting source of flavonoids because of its exact content in quercetin, apigenin, chrysin and scutellarin as well as in baicalin. These flavonoids exert vasoprotective properties and many activities such as: anti-oxidative via several pathways, anti-in-flammatory, anti-ischaemic, cardioprotective and anti-hypertensive. However, there is lack of summaries of results of studies in context of potential and future application of flavonoids with determined composition and activity. Our review aims to provide a literature survey of in vitro, in vivo and ex vivo pharmacological studies of selected flavonoids (apigenin, chrysin and scutellarin, baicalin) in various models of hypertension carried out in 2008–2018.

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Scutellarin protects against vascular endothelial dysfunction and prevents atherosclerosis via antioxidation

Cited by 67 publications

References 30 publications

Luteolin Prevents Cardiometabolic Alterations and Vascular Dysfunction in Mice With HFD-Induced Obesity

Luteolin Prevents Cardiometabolic Alterations and Vascular Dysfunction in Mice With HFD-Induced Obesity

<p>Metformin Activates the AMPK-mTOR Pathway by Modulating lncRNA <em>TUG1</em> to Induce Autophagy and Inhibit Atherosclerosis</p>

In vitro and in vivo activities of flavonoids – apigenin, baicalin, chrysin, scutellarin – in regulation of hypertension – a review for their possible effects in pregnancy-induced hypertension

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