SDF-1/CXCR4 promotes F5M2 osteosarcoma cell migration by activating the Wnt/β-catenin signaling pathway

Lu, Yao; Guan, Guofeng; Chen, Jie; Wang, Chunqiu; Ma, Qiang; Yuan, Wen; Qiu, Xiuchun; Zhang, Xiaoping; Zhou, Yong

doi:10.1007/s12032-015-0576-0

Cited by 12 publications

(8 citation statements)

References 35 publications

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“…In the present study, the suppression of CXCR4 expression also decreased the protein expression of β-catenin targeting genes (MMP-2 and c-myc). Our results confirmed the previously established role of the CXCR4/β-catenin axis in the malignant progression of cancers (18)(19)(20).…”

Section: Discussionsupporting

confidence: 91%

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Suppression of the SDF‑1/CXCR4/β‑catenin axis contributes to bladder cancer cell growth inhibition in�vitro and in�vivo

Zhang

Yang

et al. 2018

Oncol Rep

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Previous studies have found that the activation of stromal cell‑derived factor‑1 (SDF‑1)/CXC chemokine receptor‑4 (CXCR4)/β‑catenin signaling is associated with biological malignant potential in cancers. However, its function has been rarely reported in the progression of bladder cancer (BCa). The aim of the present study was to investigate the association of SDF‑1/CXCR4 signaling and β‑catenin in regards to BCa cell proliferation, colony formation, migration and invasion. The methods used were MTS, colony formation, and Transwell migration and invasion assays which were performed in SW780 cells following treatment with the CXCR4 antagonist AMD3465, SDF‑1, the β‑catenin antagonist FH535, AMD3465+SDF‑1 or FH535+SDF‑1. The mRNA and protein levels were assayed by RT‑qPCR and western blotting, respectively. The effect of AMD3465 on SW780 cell xenograft growth in vivo was evaluated using a nude mouse model. According to our results, human BCa SW780 cells were identified as having high expression of CXCR4 and β‑catenin. Subsequently, we found that both CXCR4 and β‑catenin antagonists could significantly inhibit the proliferation, colony formation, migration and invasion of SW780 cells. Notably, SDF‑1 could reverse the inhibitory effects of AMD3465 and FH535 on proliferation, colony formation, migration and invasion in SW780 cells. In AMD3465‑treated SW780 cells, the expression of c‑myc was significantly upregulated, and E‑cadherin was downregulated in the presence of SDF‑1. Furthermore, the tumor volume and average weight in the AMD3465‑treated group were evidently less than these parameters in the control group, indicating that AMD3465 can inhibit SW780 cell growth in vivo. In conclusion, targeting the SDF‑1/CXCR4/β‑catenin axis may be a potential therapeutic target for suppressing BCa progression.

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Section: Discussionsupporting

confidence: 91%

“…Previous studies have demonstrated that SDF-1/CXCR4 may induce cancer cell proliferation, and invasion through activation of the Wnt/β-catenin signaling pathway (18)(19)(20). Specifically, the Wnt/β-catenin signaling pathway is a key modulator of cellular proliferation (21).…”

Section: Introductionmentioning

confidence: 99%

Suppression of the SDF‑1/CXCR4/β‑catenin axis contributes to bladder cancer cell growth inhibition in�vitro and in�vivo

Zhang

Yang

et al. 2018

Oncol Rep

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“…The PI3K/Akt and mitogen-activated protein kinase (MAPK)/ERK signal transduction pathways, which are mediated by SDF-1, contribute to cell migration, proliferation, tube formation, apoptosis and chemotaxis (26,27). For example, SDF-1-induced Akt and ERK activation results in lung cancer cell invasion and metastasis (28), sacral chondrosarcoma and glioblastoma cell cycle progression and epithelial-mesenchymal transition (29,30), as well as ovarian cancer cell proliferation (31), and pre-B (26), F5M2 osteosarcoma (32) and epitheloid carcinoma (33) cell migration. It has been reported that SDF-1 serves a critical role in the regulation of EPC cellular functions, including cell proliferation and migration (23,34).…”

Section: Introductionmentioning

confidence: 99%

Role of the stromal cell derived factor‑1 in the biological functions of endothelial progenitor cells and its underlying mechanisms

et al. 2020

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Stromal cell derived factor-1 (SDF-1) is a chemokine that plays a critical role in the homing of stem and progenitor cells, including endothelial progenitor cells (EPCs). However, little research has been undertaken to evaluate the roles of SDF-1 in the biological functions of EPCs and related signaling pathways. The present study aimed to investigate the biological functions of EPCs in response to SDF-1, as well as the underlying mechanisms. The effects of SDF-1 treatment on EPC proliferation, migration and tube formation were assessed by performing MTS, Transwell and in vitro tube formation assays, respectively. The phosphorylation status of Akt and ERK was evaluated by western blotting. The present results indicated that SDF-1 treatment enhanced EPC proliferation, migration and tube formation compared with the control group. Furthermore, SDF-1-induced EPC proliferation was significantly reduced following treatment with a C-X-C Motif Chemokine Receptor 4 antagonist (AMD3100), a PI3K inhibitor (LY294002) and the mitogen-activated protein kinase kinase inhibitor (MEK; PD98059). SDF-1-induced migration and angiogenesis were significantly suppressed by the PI3K inhibitor, but not the MEK inhibitor. Moreover, SDF-1 significantly increased the protein expression levels of phosphorylated (p)-Akt and p-ERK; however, SDF-1-induced effects on protein expression were suppressed by AMD3100, LY294002 and PD98059. Thus, SDF-1-induced EPC proliferation was mediated by activation of the Akt and ERK signaling pathways, whereas SDF-1-mediated EPC migration and tube formation only involved activation of the Akt signaling pathway.

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“…CXCL12 is also called stromal cell-derived factor-1 ( SDF-1) and can bind to G-protein-coupled chemokine receptor CXCR4 ( Nagasawa, 2014 ). Increasing studies suggested that CXCL12 /CXCR4 axis played pivotal roles in tumor growth and development ( Balkwill, 2004 ; Lu et al., 2015 ; Perissinotto et al, 2005 ). Li et al (2018) .…”

Section: Discussionmentioning

confidence: 99%

Identification of potential gene signatures associated with osteosarcoma by integrated bioinformatics analysis

Jia¹,

Liu²,

Han

et al. 2021

PeerJ

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Background Osteosarcoma (OS) is the most primary malignant bone cancer in children and adolescents with a high mortality rate. This work aims to screen novel potential gene signatures associated with OS by integrated microarray analysis of the Gene Expression Omnibus (GEO) database. Material and Methods The OS microarray datasets were searched and downloaded from GEO database to identify differentially expressed genes (DEGs) between OS and normal samples. Afterwards, the functional enrichment analysis, protein–protein interaction (PPI) network analysis and transcription factor (TF)-target gene regulatory network were applied to uncover the biological function of DEGs. Finally, two published OS datasets (GSE39262 and GSE126209) were obtained from GEO database for evaluating the expression level and diagnostic values of key genes. Results In total 1,059 DEGs (569 up-regulated DEGs and 490 down-regulated DEGs) between OS and normal samples were screened. Functional analysis showed that these DEGs were markedly enriched in 214 GO terms and 54 KEGG pathways such as pathways in cancer. Five genes (CAMP, METTL7A, TCN1, LTF and CXCL12) acted as hub genes in PPI network. Besides, METTL7A, CYP4F3, TCN1, LTF and NETO2 were key genes in TF-gene network. Moreover, Pax-6 regulated four key genes (TCN1, CYP4F3, NETO2 and CXCL12). The expression levels of four genes (METTL7A, TCN1, CXCL12 and NETO2) in GSE39262 set were consistent with our integration analysis. The expression levels of two genes (CXCL12 and NETO2) in GSE126209 set were consistent with our integration analysis. ROC analysis of GSE39262 set revealed that CYP4F3, CXCL12, METTL7A, TCN1 and NETO2 had good diagnostic values for OS patients. ROC analysis of GSE126209 set revealed that CXCL12, METTL7A, TCN1 and NETO2 had good diagnostic values for OS patients.

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SDF-1/CXCR4 promotes F5M2 osteosarcoma cell migration by activating the Wnt/β-catenin signaling pathway

Cited by 12 publications

References 35 publications

Suppression of the SDF‑1/CXCR4/β‑catenin axis contributes to bladder cancer cell growth inhibition in�vitro and in�vivo

Suppression of the SDF‑1/CXCR4/β‑catenin axis contributes to bladder cancer cell growth inhibition in�vitro and in�vivo

Role of the stromal cell derived factor‑1 in the biological functions of endothelial progenitor cells and its underlying mechanisms

Identification of potential gene signatures associated with osteosarcoma by integrated bioinformatics analysis

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