SDF2L1 Inhibits Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma

Zhang, Liqian; Zhang, Zunni; Qin, Liuqun; Shi, Xiang; Su, Qingde; Mo, Wuning

doi:10.1155/2020/1970936

Cited by 8 publications

(8 citation statements)

References 22 publications

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“…After incubation of the tissues with anti-RPL14(eL14) overnight, the tissue specimens were incubated with biotin-labeled goat anti-rabbit secondary antibody at room temperature for 30 minutes, followed by DAB color development for 5 min and hematoxylin staining for 1 minute. The immunohistochemical staining result was judged by the cell staining intensity and the percentage of positive stained cells [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Human/eukaryotic ribosomal protein L14 (RPL14/eL14) overexpression represses proliferation, migration, invasion and EMT process in nasopharyngeal carcinoma

Zhang

Qiu

et al. 2021

Bioengineered

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Although human/eukaryotic ribosomal protein L14 (RPL14/eL14) is known to be associated with a variety of cancers, its role in nasopharyngeal carcinoma (NPC) remains unclear. The aim of this study was to explore the impact of RPL14(eL14) in NPC. The results of quantitative real-time polymerase chain reaction (qRT-PCR), western blot, and immunohistochemical staining revealed that the expression of RPL14(eL14) significantly reduced in NPC tissues and cells. Furthermore, the protein expression of RPL14(eL14) was linked to NPC-related clinical pathological features, including the T and N classification of Tumor Node Metastasis (TNM) staging (all p < 0.05). Cell counting kit-8 (CCK-8) assay and colony formation assay revealed that RPL14(eL14) overexpression repressed NPC cell proliferation. In cell cycle assay, RPL14(eL14) overexpression significantly blocked NPC cells in S phase. Overexpression of RPL14(eL14) repressed cell migration and invasion in NPC as shown by transwell assay and cell scratch healing assay. In addition, RPL14(eL14) was closely correlated with the expression of epithelial-mesenchymal transition (EMT) biomarkers, including E-cadherin, N-cadherin, and vimentin as detected by western blot. In conclusion, our results revealed that RPL14(eL14) may be considered as an antioncogene in NPC, which greatly suppresses cancer progression.

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Section: Methodsmentioning

confidence: 99%

Human/eukaryotic ribosomal protein L14 (RPL14/eL14) overexpression represses proliferation, migration, invasion and EMT process in nasopharyngeal carcinoma

Zhang

Qiu

et al. 2021

Bioengineered

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“…SHCBP1 is known as a member of Shc family, which has been found to be important in the regulation of apoptosis and drug resistancein mammalian cells [36,39,40] . Dysreglation of Shcs, leading to uncontrolled proliferation, is a characteristic of human cancers.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of SHCBP1 predicts poor prognosis of gastric cancer

Zhang²,

Sun³

et al. 2022

Preprint

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Background SHC SH2-domain binding protein 1 (SHCBP1) functions as an oncogene in different cancers. The effect pattern of SHCBP1 in gastric cancer (GC) tissues and the relationships between SHCBP1 expression and GC progression were explored in our study. Methods The expression of SHCBP1 in GC tissues and corresponding adjacent tissues was analyzed by immune histochemistry, Western blot and RT-PCR. With bioinformatics methods, the associations between the expression level of SHCBP1 and different cancer progression were also analyzed. In addition, the knockdown of SHCBP1 gene using shRNA transfection was applied to investigate the effect of SHCBP1 expressionon the proliferation, migration, invasion and epithelial to mesenchymal transition (EMT) capabilities of GC cells. Results The expression level of SHCBP1 mRNA was significantly increased in GC tissues compared with that of the adjacent tissues (P < 0.05). The expressions of SHCBP1 in GC tissues were associated with T stage and TNM stage. High SHCBP1 expression and TNM stage were independent factors to predict poor prognosis for GC patients.With the bioinformatics database, SHCBP1 could be treated as an oncogene of GC. And the in vitro study further confirmed that the knockdown of SHCBP1 expression in GC cells effectively suppressed the proliferation, migration, invasion and EMT capabilities of GC cells. Conclusions Our results indicate that SHCBP1 promote the metastasis of GC and could be served as potential prognostic marker and potential molecular target for GC therapy.

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“…Unfortunately, the early diagnosis method of NPC still has its limitations in clinical practice, resulting in the majority cases of NPC being diagnosed at the advanced stage, which often leads to some regrettable outcomes. Therefore, early diagnosis of NPC is of great importance for disease control, finding new disease-related biomarkers for the early diagnosis of NPC is ever-increasing necessary [9,10]. Until now, MS has been extensively applied in proteomics analysis with the advantages of rapid analysis speed and high sensitivity [11,12].…”

Section: Introductionmentioning

confidence: 99%

Bi‐amino acid functionalized biomimetic honeycomb chitosan membrane as a multifunctional hydrophilic probe for specific capture of N‐linked glycopeptides in nasopharyngeal carcinoma's disease patient's serum

Wang

et al. 2022

J of Separation Science

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In this work, a novel porous bifunctionalized composite material was synthesized via a simple method. Gold nanoparticles are uniformly dispersed on the surface of the biomimetic honeycomb chitosan membrane through the interaction between amino and Au, and then cysteine and glutathione are successfully grafted onto the surface of the Au by the Au‐S bond. The modification of cysteine and glutathione makes this bifunctionalized composite material have significant advantages of superhydrophilicity and small steric hindrance simultaneously. This material manifests excellent property in glycopeptides enrichment, with high selectivity (1:5000), low detection limit (0.1 fmol·μL–1), high recovery rate (99.4 ± 0.5%), and good repeatability. In addition, with the help of nano‐flow liquid chromatography tandem mass spectrometry, this composite achieved excellent performance in efficiently enriching glycopeptides in the serum of healthy people and nasopharyngeal carcinoma's disease patient. More excitingly, further gene ontology analysis of molecular function and biological process indicated that 41 original glycoproteins of the identified glycopeptides from serum of nasopharyngeal carcinoma's disease patient significantly partake in numerous cancer‐associated events, including protease binding, calcium ion binding, enzyme binding, extracellular matrix organization, cellular response to tumor necrosis factor, and inflammatory response.

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SDF2L1 Inhibits Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma

Cited by 8 publications

References 22 publications

Human/eukaryotic ribosomal protein L14 (RPL14/eL14) overexpression represses proliferation, migration, invasion and EMT process in nasopharyngeal carcinoma

Human/eukaryotic ribosomal protein L14 (RPL14/eL14) overexpression represses proliferation, migration, invasion and EMT process in nasopharyngeal carcinoma

Overexpression of SHCBP1 predicts poor prognosis of gastric cancer

Bi‐amino acid functionalized biomimetic honeycomb chitosan membrane as a multifunctional hydrophilic probe for specific capture of N‐linked glycopeptides in nasopharyngeal carcinoma's disease patient's serum

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