SDS-PAGE comparative studies on the polyhedral and viral polypeptides of the nuclear polyhedrosis viruses of Mamestra brassicae, Autographa californica, and Lymantria dispar

Maskos, C; Miltenburger, H.G.

doi:10.1016/0022-2011(81)90072-0

Cited by 18 publications

(16 citation statements)

References 21 publications

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“…The 30.9 kDa protein, first detected at 16 h p.i., was one of the more prominent proteins detected. This protein is similar to the polyhedrin, in size and expression pattern, described in MMNPV (Maskos and Miltenburger, 1981;Singh et al, 1983), and possibly corresponds to the LdMNPV 30.5 kDa protein suggested to be polyhedrin by McClintock et al, (1986). The other most prominent proteins identified were the 14.5, 40.7, 56.2, and 58.9 kDa proteins.…”

Section: Identification Of Viral Specific Proteinssupporting

confidence: 54%

“…Previous work on ~MNPV has focused on genome cloning and restriction enzyme mapping (Smith et al, 1988;McClintock and Dougherty, 1988), characterization of the polyhedrin gene (Smith et al, 1988), and identification of viral proteins (Padhi et al, 1974;Maskos and Miltenburger, 1981;Singh et al, 1983;Stiles et al, 1983;McClintock et al, 1986). The results of the present analysis provide a spatial and temporal characterization of LdMNPV gene expression, thus extending the knowledge of genome organization within the baculoviridae.…”

Section: Discussionmentioning

confidence: 99%

“…Polypeptides that compose LdMNPV capsids and viral proteins expressed in cell culture have been previously described (Padhi et al, 1974;Maskos and Miltenburger, 1981;Singh et al, 1983;Stiles et al, 1983;McClintock et al, 19861, and LdMNPV genomic restriction maps constructed (McClintock and Dougherty, 1988;Smith et al, 1988). We characterized LdMNPV gene expression through the generation of transcription and translation maps.…”

Section: Introductionmentioning

confidence: 99%

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Temporal analysis and spatial mapping of Lymantria dispar nuclear polyhedrosis virus transcripts and in vitro translation polypeptides

Slavicek

1991

Virus Research

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SummaryGenomic expression of the Lymantriu dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) was studied. Viral specific transcripts expressed in cell culture at various times from 2 through 72 h postinfection were identified and their genomic origins mapped through Northern analysis. Sixty-five distinct transcripts were identified in this analysis. Most viral transcripts were expressed late in infection, and originated from throughout the viral genome. Viral polypeptides expressed in infected 652Y cells were labeled with [35S]methionine and identified by autoradiography after separation by SDS polyacrylamide gel electrophoresis. Viral protein synthesis was found to occur in a sequential manner. Four proteins were identified in the early phase of viral replication (4-12 h p.i.), 24 proteins in the intermediate phase (12-24 h p.i.), and 5 proteins during the late phase ( > 24 h p.i.1. Gytoplasmic RNAs were isolated from LdMNPV infected cells at 16, 24, and 48 h p.i., and used for hybrid selections with overlapping DNA fragments that covered the entire MMNPV genome. The selected RNAs were translated in vitro, and 61 distinct viral polypeptides were identified and their genomic origins mapped. Temporal and spatial transcription and translation maps of the LdMNPV genome were generated with these data, and the expression pattern of the MMNPV genome was compared to that of the Autograph culifornicu nuclear polyhedrosis virus.

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Section: Identification Of Viral Specific Proteinssupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Temporal analysis and spatial mapping of Lymantria dispar nuclear polyhedrosis virus transcripts and in vitro translation polypeptides

Slavicek

1991

Virus Research

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“…In addition, because AcMNPV and RoMNPV polyhedrins can be distinguished by partial proteolysis (Maskos & Miltenburger, 1981), we also used polyhedrin as a marker for investigating recombination. This paper presents the results of these investigations.…”

Section: Introductionmentioning

confidence: 99%

Recombination of Autographa californica and Rachiplusia ou Nuclear Polyhedrosis Viruses in Galleria mellonella L.

Croizier¹,

Croizier²,

Quiot³

et al. 1988

Journal of General Virology

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“…Samples were boiled for 5 min in sample buffer (5~o 2-mercaptoethanol, 2~ SDS, 0.063 M-Tris-HC1 buffer pH 6.8, 10~ glycerol, and 0.001 ~ bromophenol blue; Maskos & Miltenburger, 1981). Polypeptides were separated by discontinuous SDS-PAGE in a slab gel apparatus (dimensions 10 x 14 x 0.15 cm) using a 3~ stacking and an 11 ~ separating gel (Laemmli, 1970).…”

Section: Polyacrylamide Gel Electrophoresis (Page)mentioning

confidence: 99%

Choristoneura murinana Nuclear Polyhedrosis Virus: Comparative Biochemical and Biological Examination of Replication in vivo and in vitro

Naser

Harvey

Huger

et al. 1987

Journal of General Virology

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SUMMARYAn in vitro replication system for the Choristoneura murinana nuclear polyhedrosis virus (CmMNPV) was established and used (i) to characterize this baculovirus biochemically; (ii) to study the cytoplasmic spindle-shaped inclusions (CSIs) associated with CmMNPV replication; and (iii) to compare the cytopathic changes during CmMNPV replication in vivo and in vitro as well as the properties of virions, polyhedra and CSIs from both systems. It was shown that the processes occurring during, and the products of, CmMNPV replication in vitro closely resemble those in vivo, i.e. in larval hosts. Genome analysis by restriction endonucleases, as well as infectivity studies with polyhedra from both sources did not reveal major differences between virus produced in vivo and that produced in vitro. The CSIs were found exclusively in the cytoplasm of infected cells and were shown to consist of a single protein of Mr 50000. Although the biological significance of these spindles, which are produced in large quantities, is not known, they do not seem to be of importance for the infectivity of this baculovirus in vivo.

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SDS-PAGE comparative studies on the polyhedral and viral polypeptides of the nuclear polyhedrosis viruses of Mamestra brassicae, Autographa californica, and Lymantria dispar

Cited by 18 publications

References 21 publications

Temporal analysis and spatial mapping of Lymantria dispar nuclear polyhedrosis virus transcripts and in vitro translation polypeptides

Temporal analysis and spatial mapping of Lymantria dispar nuclear polyhedrosis virus transcripts and in vitro translation polypeptides

Recombination of Autographa californica and Rachiplusia ou Nuclear Polyhedrosis Viruses in Galleria mellonella L.

Choristoneura murinana Nuclear Polyhedrosis Virus: Comparative Biochemical and Biological Examination of Replication in vivo and in vitro

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