SDS-PAGE for Monitoring the Dissolution of Zinc Oxide Bactericidal Nanoparticles (Zinkicide) in Aqueous Solutions

Untracht, Zachary; Özcan, Ali; Santra, Swadeshmukul; Kang, Hyeran

doi:10.1021/acsomega.9b02893

Cited by 6 publications

(3 citation statements)

References 36 publications

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“…Due to its biocompatibility, safety, and activity against a broad spectrum of microorganisms, ZnO’s antibacterial activity has been well-investigated both in the presence (UV and visible) and absence of light. The mechanism of antibacterial activity of ZnO in the presence of light, studied by many researchers, is attributed to oxidative stress due to reactive oxygen species (ROS) [ 20 ], dissolution of zinc ions [ 21 ] and internalization of NPs leading to cell death [ 22 ]. Among these, ROS is considered to be the dominant mechanism.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Photocatalytic Properties of ZnO Nanoparticles Obtained from Chemical versus Saponaria officinalis Extract-Mediated Synthesis

et al. 2021

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In the present work, the properties of ZnO nanoparticles obtained using an eco-friendly synthesis (biomediated methods in microwave irradiation) were studied. Saponaria officinalis extracts were used as both reducing and capping agents in the green nanochemistry synthesis of ZnO. Inorganic zinc oxide nanopowders were successfully prepared by a modified hydrothermal method and plant extract-mediated method. The influence of microwave irradiation was studied in both cases. The size, composition, crystallinity and morphology of inorganic nanoparticles (NPs) were investigated using dynamic light scattering (DLS), powder X-ray diffraction (XRD), SEM-EDX microscopy. Tunings of the nanochemistry reaction conditions (Zn precursor, structuring agent), ZnO NPs with various shapes were obtained, from quasi-spherical to flower-like. The optical properties and photocatalytic activity (degradation of methylene blue as model compound) were also investigated. ZnO nanopowders’ antibacterial activity was tested against Gram-positive and Gram-negative bacterial strains to evidence the influence of the vegetal extract-mediated synthesis on the biological activity.

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Section: Introductionmentioning

confidence: 99%

Antibacterial and Photocatalytic Properties of ZnO Nanoparticles Obtained from Chemical versus Saponaria officinalis Extract-Mediated Synthesis

et al. 2021

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“…34 Their reactivity has been attributed to nanoparticles rather than to dissolved Zn 2+ ions, 24 albeit the dissolution of nano-ZnO is observed at low concentrations. 76 Previous studies have established the ability of ZnO nanoparticles to generate superoxides ( • O 2 − ). 77,78 Here, we have shown the generation of hydroxyl ( • OH) species upon degradation of salicylic acid and dissociation of water.…”

Section: Discussionmentioning

confidence: 99%

Role of Capping Agents in the Synthesis of Salicylate-Capped Zinc Oxide Nanoparticles

Rawal

Smith

Özcan

et al. 2020

ACS Appl. Nano Mater.

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Capping agents are often used for controlling the size, aggregation, and properties of nanoparticles. To guide the design of improved nanomaterials for targeted performance, one can use mechanistic insights into the interactions between capping agents and nanoparticles. Here, we employ density functional theory (DFT), reactive force-field molecular dynamics (ReaxFF MD) simulations, and optical spectroscopy to study the interactions between salicylate, as a model capping agent, and zinc oxide (ZnO) nanoparticles. We find that salicylate strongly interacts with the nanoparticle via the formation of O−Zn bonds in a distorted six-membered coordination ring structure. We describe the mechanisms of capping of ZnO nanoparticles by salicylate via three different binding modes. Simulations indicate that salicylate undergoes dissociative adsorption at the highly active surface Zn sites via a hydrogen-transfer process, thereby forming a tridentate configuration. The water-mediated interaction also facilitates the dissociative adsorption, leading to two salicylate O atoms coordinating with a surface Zn atom, while the other salicylate O atom bonds with another surface Zn atom. For molecular adsorption, binding free energies indicate that salicylate binds more strongly to ZnO, often in a bidentate configuration, than water does. The formation of the salicylate−ZnO complex is substantiated by UV−visible and Fourier transform infrared spectra. We find that the CO stretching mode of salicylate becomes softened when it interacts with the nanoparticle, suggesting chemisorption of salicylate on ZnO. Although DFT predicts strong interaction between salicylate and ZnO, ReaxFF MD simulation indicates the moderate interaction between these two components in aqueous solution. Water molecules in close contact with the nanoparticle surface undergo dissociation, thus resulting in a surface hydroxyl, a reactive oxygen species that may influence the nanoparticle's catalytic properties. The atomic-level information provided here can guide the selection process of salicylate as an appropriate agent for ZnO nanoparticle synthesis when strong interactions of particles with capping agents are required.

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“…The relative amounts of protein bands were analyzed by IM-AGE LAB software (Bio-Rad) as described in Ref. [51].…”

Section: Low-speed Cosedimentation Assaymentioning

confidence: 99%

Crowding tunes the organization and mechanics of actin bundles formed by crosslinking proteins

Park

Lee

et al. 2020

FEBS Letters

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Fascin and α‐actinin form higher‐ordered actin bundles that mediate numerous cellular processes including cell morphogenesis and movement. While it is understood crosslinked bundle formation occurs in crowded cytoplasm, how crowding affects the bundling activities of the two crosslinking proteins is not known. Here, we demonstrate how solution crowding modulates the organization and mechanical properties of fascin‐ and α‐actinin‐induced bundles, utilizing total internal reflection fluorescence and atomic force microscopy imaging. Molecular dynamics simulations support the inference that crowding reduces binding interaction between actin filaments and fascin or the calponin homology 1 domain of α‐actinin evidenced by interaction energy and hydrogen bonding analysis. Based on our findings, we suggest a mechanism of crosslinked actin bundle assembly and mechanics in crowded intracellular environments.

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SDS-PAGE for Monitoring the Dissolution of Zinc Oxide Bactericidal Nanoparticles (Zinkicide) in Aqueous Solutions

Cited by 6 publications

References 36 publications

Antibacterial and Photocatalytic Properties of ZnO Nanoparticles Obtained from Chemical versus Saponaria officinalis Extract-Mediated Synthesis

Antibacterial and Photocatalytic Properties of ZnO Nanoparticles Obtained from Chemical versus Saponaria officinalis Extract-Mediated Synthesis

Role of Capping Agents in the Synthesis of Salicylate-Capped Zinc Oxide Nanoparticles

Crowding tunes the organization and mechanics of actin bundles formed by crosslinking proteins

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