SDS-PAGE Protein and HPTLC Polyphenols Profiling as a Promising Tool for Authentication of Goldenrod Honey

Dżugan, Małgorzata; Miłek, Michał; Kielar, Patrycja; Stępień, Karolina; Sidor, Ewelina; Bocian, Aleksandra

doi:10.3390/foods11162390

Cited by 11 publications

(8 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on the examination of the physicochemical parameters of the samples, the electrical conductivity, which is closely related to the pH and macro-and microelement content, proved to be constant over the years, and several research groups measured values similar to our results 47,48,49,50 . However, we experienced a change in the color intensity of the varietal honeys as the storage time increased, and we observed a darkening of the color of the samples.…”

Section: Discussionsupporting

confidence: 83%

Antibacterial activity of Hungarian varietal honeys changes over time against respiratory pathogens

Nagy-Radványi,

Balázs,

Kocsis

et al. 2024

Preprint

View full text Add to dashboard Cite

Today, antibiotic therapies that previously worked well against certain bacteria due to their natural sensitivity, are becoming less effective. Honey has been proven to inhibit the biofilm formation of some respiratory bacteria, however few data are available on how the storage time affects the antibacterial effect. The activity of black locust, goldenrod, linden and sunflower honeys from three consecutive years was analyzed against Gram-negative (Haemophilus influenzae, H. parainfluenzae, Pseudomonas aeruginosa) and Gram-positive (Streptococcus pneumoniae) bacteria using in vitro microbiological methods. After determining the physicochemical parameters of honey, broth microdilution was applied to determine the minimum inhibitory concentration of each honey type against each bacterium, and crystal violet assay was used to test their antibiofilm effect. The possible mechanism of action was explored with membrane degradation test, while structural changes were illustrated with scanning electron microscopy. As the storage time increased, the color of honeys became darker, and their antibacterial activity decreased significantly. The most remarkable inhibitory effect was exerted by linden and sunflower honeys, and P. aeruginosa proved to be the most resistant bacterium. Based on our results, honey intended for medicinal purposes should be used as fresh as possible during a treatment.

show abstract

Section: Discussionsupporting

confidence: 83%

Antibacterial activity of Hungarian varietal honeys changes over time against respiratory pathogens

Nagy-Radványi,

Balázs,

Kocsis

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Total phenolic and total flavonoid contents of PE were determined as described by Miłek et al (2022b), while total phenolic acid content according to Kulichova et al (2019). Antioxidant properties of propolis extracts were determined using DPPH, FRAP, as described by Miłek et al (2022b) and CUPRAC (Dżugan et al, 2022) protocols. The results were expressed per g of raw propolis.…”

Section: Bioactive Compound Content and Antioxidant Propertiesmentioning

confidence: 99%

Quality of Propolis Commercially Available on Podkarpacki Beekeeping Market

Miłek,

Ciszkowicz,

Zaguła

et al. 2024

Journal of Apicultural Science

Self Cite

View full text Add to dashboard Cite

Eight samples of propolis commercially available on south-eastern Poland’s beekeeping market were compared in terms of quality, chemical composition and biological activity, including antioxidant and antimicrobial properties. The organoleptic characteristics and physicochemical requirements indicated that only 50% of tested propolis samples was classified to class II regarding obligatory limits, while the others were out-class. A big variation in mineral composition was determined by the ICP-OES method but all samples were free of heavy metals (Pb, Cd and Ni). Although mesophilic bacteria, yeasts and moulds occurred in tested propolis (in general lower than 102 CFU/g), E. coli and spore-forming sulphite-reducing bacteria as well as Salmonella and Shigella bacteria were not detected. High antioxidant activity and total phenolic content for all samples was confirmed, which was correlated with antibacterial activity tested against E. coli ATCC 11775 strain. Additionally, the propolis sample with the strongest antibacterial activity (MIC 0.33 mg/mL) inhibited biofilm formation of S. aureus and S. epidermidis ATCC 35984 (MBIC 0.66 and 5.25 mg/mL, respectively). However, great variation in terms of antioxidant activity and HPLC polyphenolic profile were observed, and sakuranetin and pinobanksin were identified as the most effective antioxidant components. Moreover, the usefulness of ATR-FTIR spectra analysis was confirmed as a quick method for initial testing of propolis quality.

show abstract

“…One of the key markers of honey freshness is diastase activity. The storage environment and heat treatment of honey affect diastase activity [70,71].…”

Section: Physicochemical Parameters and Palynological Characteristicsmentioning

confidence: 99%

Sidr Honeys Physical and Chemical Characterization, a Comprehensive Approach through LC-MS/MS, NMR, and GC-MS Analysis

El-Wahed,

Rashwan,

AlAjmi

et al. 2023

Separations

View full text Add to dashboard Cite

Honey intake is advantageous to human health due to its antioxidant, anticancer, anti-inflammatory, and antimicrobial properties, all of which are attributed to the rich bioactive compound contents. Moreover, hepatoprotective, wound healing, and gastrointestinal protective properties have been documented. Honey’s nutritional value is significantly affected by its chemical composition, which varies depending on botanical and geographical origin. In particular, after Manuka honey, Sidr honey from the Ziziphus species is the most popular. The chemical compositions, physicochemical properties, bioactive compounds, and sensory characteristics of two Sidr honey samples from Egypt and Saudi Arabia were investigated in the current study. Moisture content, electrical conductivity (EC), pH, free acidity (FA), total acidity, lactone hydroxymethylfurfural (HMF) content, and diastase (α-amylase) activity were measured. By using high-performance liquid chromatography (HPLC), mass spectrometry (LC-MS/MS), nuclear magnetic resonance (1HNMR), and solid-phase micro-extraction (SPME) coupled with gas chromatography (GC-MS) analyses, the sugar profile, non-volatile, and volatile compounds were also identified. The physicochemical analysis revealed the following results for Sidr honey from Saudi Arabia and Egypt, respectively: a moisture content of 18.03 ± 0.05% and 19.03 ± 0.06%, EC values of 1.18 ± 0.05 and 1.16 ± 0.01 mS/cm, pH values of 4.87 ± 0.08 and 5.10 ± 0.01, FA of 37.50 ± 0.05 and 36.50 ± 0.05 meq/kg, total acidity of 41.06 ± 0.05 and 37.50 ± 0.05 meq/kg, lactone of 3.49 ± 0.005 and 1 ± 0.0 meq/kg, HMF of 20.92 ± 0.02 and 11.33 ± 0.01 mg/kg, and diastase of 59.97 ± 0.05 and 8.64 ± 0.06g/100 g. Honey from Saudi Arabia and Egypt displayed 22.51 ± 0.05 and 26.62 ± 0.16 % glucose, 40.33 ± 0.06 and 35.28 ± 0.01% fructose, 8.94 ± 0.17, and 8.87 ± 0.01% sucrose, and 8.22 ± 0.006 and 8.13 ± 0.01% maltose, respectively. According to the International Honey Commission (IHC) and GCC Standardization Organization (GSO) regulations, the levels of glucose, fructose, sucrose, and maltose were near the standard levels. Flavonoids, sugars, vitamins, and nitrogen contents were additionally measured using LC-MS/MS, whereas GC-MS was employed to identify aldehydes, ketones, phenols, acids, esters, anthraquinone, hydrocarbons, and nitrogenous compounds. The results of a study on the effect of honey’s geographic origin on its broad quality are summarized. As a result, knowing its optimal chemical and physical characteristics served as the criterion and indicator of the honey’s quality.

show abstract

SDS-PAGE Protein and HPTLC Polyphenols Profiling as a Promising Tool for Authentication of Goldenrod Honey

Cited by 11 publications

References 36 publications

Antibacterial activity of Hungarian varietal honeys changes over time against respiratory pathogens

Antibacterial activity of Hungarian varietal honeys changes over time against respiratory pathogens

Quality of Propolis Commercially Available on Podkarpacki Beekeeping Market

Sidr Honeys Physical and Chemical Characterization, a Comprehensive Approach through LC-MS/MS, NMR, and GC-MS Analysis

Contact Info

Product

Resources

About