Advanced Methods in Molecular Biology and Biotechnology 2021
DOI: 10.1016/b978-0-12-824449-4.00003-7
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SDS-polyacrylamide gel electrophoresis

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“…The technique involves appropriate sample preparation, electrophoretic separation (i.e. SDS-PAGE – sodium dodecyl sulphate polyacrylamide gel electrophoresis) [ 7 , 59 , 60 ], transfer of separated proteins from the gel to the membrane (nitrocellulose, polyvinylidene difluoride (PVDF)) [ 61 , 62 ], incubation with appropriate antibodies, and detection [ 63 ]. Visualisation of these markers can be carried out colorimetrically or with chemiluminescence methods [ 53 , 57 ].…”
Section: Immunocytochemical Methods For Qualitative and Quantitative ...mentioning
confidence: 99%
“…The technique involves appropriate sample preparation, electrophoretic separation (i.e. SDS-PAGE – sodium dodecyl sulphate polyacrylamide gel electrophoresis) [ 7 , 59 , 60 ], transfer of separated proteins from the gel to the membrane (nitrocellulose, polyvinylidene difluoride (PVDF)) [ 61 , 62 ], incubation with appropriate antibodies, and detection [ 63 ]. Visualisation of these markers can be carried out colorimetrically or with chemiluminescence methods [ 53 , 57 ].…”
Section: Immunocytochemical Methods For Qualitative and Quantitative ...mentioning
confidence: 99%