SDZ-induced apoptosis in lovo cells

Abstract

Colorectal cancer (CRC) is one of the most common malignancies occurring in men and women in China, and is associated with a high rate of incidence and mortality [1] . Approximately more than 50% of patients present with advanced disease. Diagnosis of CRC at a later stage results in 5-year survival rates of 67% in patients whose cancer has spread to regional lymph nodes, and 10%-30% for those in whom CRC has spread to distant organs such as the liver [2] .For patients with metastatic disease, surgery has a limited role, because only when it allows a complete resection of organ metastases does it have a relevant impact on survival of patients. In the remaining cases, others therapy such as chemotherapy, although not curative, may increase the time to disease progression and the overall survival.SDZ was the compound prescription medicine, which composed of ganoderma applanatum, ginseng, astragalus mongholicus and so on. Formerly studied indicated SDZ had the good anti-cancer functions [3] . But its mechanism is unclear. The apoptosis induced by SDZ was observed so that verifies the anti-cancer mechanism of SDZ. Methods Fluorescent stainingThe cells, which were logarithmic growth phase, was cultured with SDZ solution which final concentration was 1000 μg/mL for three days. The partes aequales physiological saline was added for controls. Positive control group with 5-FU. One group of culture cells were obtained everyday. The cells were dyed (the staining solution composed of Hoechst, PI and buffer) after the cells were washed with PBS. The apoptosis was observed under the fluorescence microscope. Transmission electron microscopeThe cells, which were cultured with SDZ for three days, were treated according to routine. The ultramicrostructure was observed with transmission electron microscope. Results Fluorescent stainingThe trachychromatic blue apoptosis cells were observed after the lovo cell was treated with SDZ for 24 h (Fig. 1). The rate of apoptosis was 30.2%. Apoptosis rate of positive control group with 5-FU was 33.1%. Meanwhile, the nuclear fragmentation and cell minification were observed. The rate of apoptosis was 1.1%. The apoptosis have significant difference between experiment and negative group (P < 0.05). The apoptosis have no significant difference between experiment and positive group (P > 0.05).Abstract Objective: To explore the inhibition effective of the SDZ on lovo cell growth of colon cancer in vitro. Methods: The apoptosis was observed by Hoechst fluorescein stain and transmission electron microscope. Results: The apoptosis was observed after the lovo cells treated by SDZ (1000 μg/mL) for 24 h. The rate of apoptosis was 30.2%. Conclusion: The apoptosis of lovo cells can be induced by SDZ in vitro.