The body conditions indices were useful to determine an individual's well-being, and favorable food availability indicating a good environmental condition. The conditions of the mangrove horseshoe crab (Carcinoscorpius rotundicauda) might be related to several environmental parameters. The study's aim was to analyze the key environmental parameters affecting the body condition of C. rotundicauda found in Banyuasin Estuary Waters. The sampling was conducted in July 2019 in Banyuasin Estuary Waters. The data of weight and prosomal width for C. rotundicauda were used to estimate the body conditions indices (relative condition factor). While the environmental parameters data were recorded for each sampling site. The backward stepwise regression was used to determine the key environmental parameters affecting the body condition indices. The best-fitted model (adjusted R2 = 91.9%; F = 60.102; p < 0.05) indicated several environmental parameters (salinity, pH, seafloor temperature, clay and silt contents) significantly affecting the body condition indices. Changes in the key environmental parameters could generate changes in the availability of favorable food for C. rotundicauda. The result also can be used as baseline data for determining the marine conservation areas as well as horseshoe crab management plan.