Seabuckthorn polysaccharide combined with astragalus polysaccharide ameliorate alcoholic fatty liver by regulating intestinal flora

Abstract

BackgroundAt present, the incidence of alcoholic fatty liver disease (AFLD) is increasing year by year, and numerous studies have confirmed that liver diseases are closely related to intestinal flora. Seabuckthorn and Astragalus membranaceus, as traditional Chinese medicine (TCM) with the homology of medicine and food, have good liver protection, and their polysaccharides can regulate the intestinal flora. Here, we studied the effects of HRP, APS and the combination of the two polysaccharides on the intestinal flora of AFLD mice, which provided scientific basis for the treatment of AFLD with the two polysaccharides.Materials and methodsThirty Kunming (KM) mice were randomly divided into the control group (Con), the model group (Mod), the HRP treatment group (HRP), the APS treatment group (APS), and HRP+APS treatment group (HRP+APS), with six mice in each group. The AFLD model was constructed by continuous intragastric administration of 42% vol Niulanshan ethanol solution for 28 days, and the mice in each polysaccharide group were given corresponding drugs. The levels of AST, ALT, TC and TG in serum of mice were measured. 16S rRNA amplicon sequencing technique was used to determine the diversity and richness of intestinal flora, and the relative abundance of intestinal flora at phylum level and genus level of the mice in each group.ResultsHRP, APS and HRP+APS could reduce the serum levels of AST, ALT, TC and TG in mice. In addition, HRP, APS and HRP + APS restored the diversity, relative abundance and community structure of intestinal mucosa bacteria in AFLD mice to a certain extent. Specifically, HRP, APS and HRP+APS remarkably decreased the ratio of Firmicutes to Bacteroidetes, and ultimately increased the abundance of beneficial bacteria and reduced the abundance of pathogenic bacteria.ConclusionHRP, APS, and HRP+APS can improve the intestinal microecology of AFLD model mice, alleviate liver injury, and maintain normal intestinal function in different degrees.

Medicinal and edible polysaccharides from ancient plants: extraction, isolation, purification, structure, biological activity and market trends of sea buckthorn polysaccharides

“…Dialysis is often used to remove small-molecule impurities from sea buckthorn crude polysaccharides. [33][34][35] With the innovation of membrane separation technology, fiber filter dialysis will become a new approach to remove small molecular impurities from polysaccharides with its advantages of fast operation and mild conditions. 36 After removing impurities such as pigments, proteins, and small molecules, further purification is required to obtain homogeneous and highly purified polysaccharides.…”

“…Dialysis is often used to remove small-molecule impurities from sea buckthorn crude polysaccharides. 33–35 With the innovation of membrane separation technology, fiber filter dialysis will become a new approach to remove small molecular impurities from polysaccharides with its advantages of fast operation and mild conditions. 36…”

“…34 Moreover, Liu et al found that SPs, Astragalus polysaccharide (APS), and SPs combined with APS treatment could improve the diversity, relative abundance, and community structure of the gut microbiota in mice with alcoholic fatty liver disease. 35…”

The potential role of alfalfa polysaccharides and their sulphated derivatives in the alleviation of obesity

“…Some plant polysaccharides can regulate the intestinal flora, alleviating alcoholic fatty liver and diabetes. 57,58 Both APs and SAPs positively influenced the regulation of the intestinal flora. APs and SAPs reduce the proportion of F/B, reducing the body's absorption of calories and alleviating obesity.…”

Construction of microneedle of Atractylodes macrocephala Rhizoma aqueous extract and effect on mammary gland hyperplasia based on intestinal flora

“…The composition and quantity of intestinal flora are in balance under normal conditions. Once the balance is broken by factors such as intestinal pH value, mental pressure, eating habits, and antibiotic use, it will cause metabolic obstacles, metabolic disorders, and even other related diseases to some extent (11)(12)(13)(14)(15)(16). The dried rhizome of the plant Atractylodes macrocephala Koidz is useful for drying and moistening water, nourishing the stomach, and strengthening the spleen.…”