Seafood cross-contamination by tetrodotoxin (TTX): management of an unusual route of exposure

Tinacci, Lara; Malloggi, Chiara; Giusti, Alice; Galli, Federico; Dall’Ara, Sonia; Armani, Andrea

doi:10.1007/s00003-023-01467-4

Cited by 1 publication

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“…sceleratus establishing itself as a new persistent invasive species in the Mediterranean, concerns arise regarding the potential risks associated with the accidental consumption of toxic pufferfish by local populations as a consequence of recreational fishing , and the possible intermingling of juveniles with other small pelagic species (e.g., anchovy, sardines, picarel and bogue) . Moreover, the risk of exposure to TTX through the consumption of common edible seafood species (originally nontoxic) that have been cross-contaminated as a result of accidental pufferfish predation cannot be ignored. , In addition, over the last few decades, shellfish has been identified as another important TTX carrier in Europe. Although TTX concentrations in shellfish are generally lower than those in pufferfish, they still pose a poisoning risk.…”

Section: Introductionmentioning

confidence: 99%

Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined with Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells

Reverté,

Rambla-Alegre,

Sanchez-Henao

et al. 2024

J. Agric. Food Chem.

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Tetrodotoxin (TTX) is a potent marine neurotoxin, responsible for numerous poisoning incidents and some human fatalities. To date, more than 30 TTX analogues have been identified, but their individual toxicities and roles in poisoning remain largely unknown. In this work, the toxicity equivalency factors (TEFs) of five TTX analogues were determined by assessing the blockade of voltage-gated sodium channels in Neuro-2a cells using automated patch clamp (APC). All TTX analogues were less toxic than TTX. The derived TEFs were applied to the individual TTX analogues concentrations measured in pufferfish samples, using liquid chromatography coupled to tandem mass spectrometry (LC−MS/MS). A comparison of these results with those obtained from APC analysis demonstrated that TEFs can be effectively used to translate LC−MS/MS analytical data into meaningful toxicological information. This is the first study to utilize APC device for the toxicological assessment of TTX analogues, highlighting its potential as a bioanalytical tool for seafood safety management and human health protection.

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