The aim of this study was to investigate the effect of grape seed extracts (GSE) at different concentrations (0, 7.5 and 15 g/L) on the melanosis formation and quality of Pacific white shrimp during 9 days of iced storage. The melanosis formation was significantly inhibited and sensory quality was significantly improved in shrimp treated by various concentration of GSE, compared with the control. The increase of pH, total volatile basic nitrogen content and the total bacteria amounts of shrimp treated by 7.5 and 15 g/L of GSE was significantly retarded. However, the melanosis score, total volatile basic nitrogen, and pH values of shrimp treated by 15 g/L of GSE was lower than that treated by 7.5 g/L of GSE. These results suggested that GSE could be used as an effective natural alternative to synthetic antimelanosic agents to inhibit postmortem melanosis and improve the quality of shrimp during iced storage.Keywords: Pacific white shrimp, melanosis, quality, grape seed extracts
IntroductionDue to the high market and nutritional value, shrimp is a very important fisher resource all over the world. However, the shelf life of shrimp was limited due to melanosis and microbiological deterioration. Melanosis or blackening, the formation of black spots in crustaceans, such as shrimp and crabs during postmortem storage, severely damage the market value and usually caused economical loss of these seafood (Kim et al., 2002). Melanosis is the result of the polymerization of phenols by polyphenoloxidase (PPO), also known as phenoloxidase, tyrosinase (EC 1.14.18.1), and cathecoloxidase (EC 1.10.3.1) (Zamorano et al., 2009).Usually, iced storage is routinely used to preserve the quality of the shrimp. However, during refrigerated or iced storage, melanosis still takes place since PPO remains active under these conditions (Gokoglu and Yerlikaya, 2008;Nirmal and Benjakul, 2010).To retard the melanosis in crustaceans, and ensure perishables have a longer shelf life, antimelanosic agents, such as 4-hexyl-1,3-benzenediol (4-hexylresorcinol), sulphite-based compounds, and phosphates, have been intensively studied and proved to be effective to inhibit melanosis (Martinez-Alverez et al., 2005Thepnuan et al., 2008). However, the use of synthetic compounds to inhibit melanosis in seafood is limited due to increasing regulatory attention and food safety concerns (McEvily et al., 1991). For example, the use of metabisulphite to inhibit melanosis of shrimp could cause the sulfur dioxide residue exceeding problems (Gomez-Guillen et al., 2005). Due to the potential health hazards of chemical additives, natural products, especially natural antioxidants and antimicrobial agents, have been intensively examined as safe alternatives to synthetic compounds (Encarnacion et al., 2010;Maqsood et al., 2013). Recently, a series of studies conducted on the utilization of natural extracts to delay melanosis formation and extend the shelf life of seafood (Nirmal and H. Sun et al. 672 Benjakul, 2011a, 2012a). The use of phenolic compounds also appear...