Seagrass Halodule pinifolia active constituent 4-methoxybenzioic acid (4-MBA) inhibits quorum sensing mediated virulence production of Pseudomonas aeruginosa

Danaraj, Jeyapragash; Mariasingarayan, Yosuva; Saravanakumar, A.; Vijayakumar, K. R.

doi:10.1016/j.micpath.2020.104392

Cited by 15 publications

(10 citation statements)

References 44 publications

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“…The low molecular weight alginate oligomer (OligoG CF-5/20), which is produced by brown seaweed Laminaria hyperborea , can act as a QS antagonist to las and rhl QS pathway expression in P. aeruginosa and to inhibit biofilm formation [ 170 ]. Studies showed that an active constituent of seagrass, Halodulepinifolia , 4-methoxybenzioic acid ( Figure 19 b), displayed inhibitory activity to QS-mediated virulence factors and biofilm formation [ 171 ]. Marine fungi also cannot be ignored as a main source.…”

Section: Qs Inhibitors From Marine Resourcesmentioning

confidence: 99%

The Molecular Architecture of Pseudomonas aeruginosa Quorum-Sensing Inhibitors

Shen

Wang

et al. 2022

Marine Drugs

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The survival selection pressure caused by antibiotic-mediated bactericidal and bacteriostatic activity is one of the important inducements for bacteria to develop drug resistance. Bacteria gain drug resistance through spontaneous mutation so as to achieve the goals of survival and reproduction. Quorum sensing (QS) is an intercellular communication system based on cell density that can regulate bacterial virulence and biofilm formation. The secretion of more than 30 virulence factors of P. aeruginosa is controlled by QS, and the formation and diffusion of biofilm is an important mechanism causing the multidrug resistance of P. aeruginosa, which is also closely related to the QS system. There are three main QS systems in P. aeruginosa: las system, rhl system, and pqs system. Quorum-sensing inhibitors (QSIs) can reduce the toxicity of bacteria without affecting the growth and enhance the sensitivity of bacterial biofilms to antibiotic treatment. These characteristics make QSIs a popular topic for research and development in the field of anti-infection. This paper reviews the research progress of the P. aeruginosa quorum-sensing system and QSIs, targeting three QS systems, which will provide help for the future research and development of novel quorum-sensing inhibitors.

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Section: Qs Inhibitors From Marine Resourcesmentioning

confidence: 99%

The Molecular Architecture of Pseudomonas aeruginosa Quorum-Sensing Inhibitors

Shen

Wang

et al. 2022

Marine Drugs

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“…From the extract of the seagrass Halodule pinifolia, 4-methoxybeanzoic acid (4-MBA) was isolated and evaluated for its potential. At the concentration of 62.5 μg/mL, it was able to prevent biofilm formation and also downregulate QS-mediated transcript and virulence factors [ 173 ].…”

Section: Marine Natural Products Against P Aeruginosamentioning

confidence: 99%

Exploring Oceans for Curative Compounds: Potential New Antimicrobial and Anti-Virulence Molecules against Pseudomonas aeruginosa

et al. 2022

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Although several antibiotics are already widely used against a large number of pathogens, the discovery of new antimicrobial compounds with new mechanisms of action is critical today in order to overcome the spreading of antimicrobial resistance among pathogen bacteria. In this regard, marine organisms represent a potential source of a wide diversity of unique secondary metabolites produced as an adaptation strategy to survive in competitive and hostile environments. Among the multidrug-resistant Gram-negative bacteria, Pseudomonas aeruginosa is undoubtedly one of the most important species due to its high intrinsic resistance to different classes of antibiotics on the market and its ability to cause serious therapeutic problems. In the present review, we first discuss the general mechanisms involved in the antibiotic resistance of P. aeruginosa. Subsequently, we list the marine molecules identified up until now showing activity against P. aeruginosa, dividing them according to whether they act as antimicrobial or anti-virulence compounds.

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“…Various solvent extracts (ethyl acetate, n-butanol, ethanol and water) of Quercusinfectoria showed down-regulation of the las and rhl genes and reduced virulence factors [23]. Moreover, a literature survey revealed that in the last decade, several medicinal, dietary crude plant extracts [22,[24][25][26][27][28] and plant-derived active secondary metabolites [29][30][31][32][33][34] had exhibited varying levels of QS and biofilm inhibition in P. aeruginosa and other Gram-negative pathogens. In our previous studies, we have investigated antiquorum sensing activity of Tinospora cordifolia and the purified phytochemicals were chemically synthesized in another research in the same lab.…”

Section: Introductionmentioning

confidence: 99%

Exploration of the inhibitory effect of Cassia fistula on quorum sensing mediated virulence factor production and biofilm activity in Pseudomonas aeruginosa: an in vivo study in model organism Caenorhabditis elegans

Peerzada

Shah

Kharkar

et al. 2023

Journal of Medical Microbiology

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Introduction. Resistance to antibiotics is leading to challenges in the treatment of microbial diseases. One amongst the various approaches to control these pathogens is quorum sensing (QS), which is used to rectify resistance issues. Blocking the bacterial QS circuit is the most reliable anti-virulence therapy to control pathogenicity-associated genes. Pseudomonas aeruginosa is a contagious bacterium that proliferates in the host by using signalling molecules like acyl-homoserine lactones; these molecules generate and disseminate toxins and virulence factors for increasing host infection. Hypothesis. The herb Cassia fistula is known to have antimicrobial, antidiabetic, anti-inflammatory, antitumor medicinal properties amongst others. We hypothesize that its crude extracts will inhibit the QS circuit of Pseudomonas aeruginosa (P. aeruginosa). Aim. The research work was aimed at evaluating anti-quorum sensing and anti-biofilm activity of various crude extracts from Cassia fistula against P. aeruginosa . Methodology. Various extraction methods and solvents were availed for maximum separation, and the extracts were screened for anti-quorum sensing activity. The most potent Fruit Ethyl acetate (FEE) extract at non-inhibitory concentrations was found to interrupt both short-chain (RhlI/R) and long-chain (LasI/R) QS circuits and other virulence factors (P<0.05) such as elastase, protease, rhamnolipids and pyocyanin levels in P. aeruginosa . Biofilm inhibitory properties of FEE were demonstrated using atomic force microscopy, scanning electron microscope and confocal laser microscope. Caenorhabditis elegans infection model (Paralytic assay) was developed to determine the protective role of FEE by reducing the pathogenicity of P. aeruginosa . Results. The study results suggest that hot crude FEE extract interfered in the QS circuit, leading to comprehensive debilitation of QS-controlled virulence factors. The extract reduced virulence factor production in P. aeruginosa at 4 mg ml-1 concentration whilst paradoxically promoting biofilm formation. Possibly, higher sugar content in the extract promoted clump formation of biofilm architecture by increasing exopolysaccharide production. Moreover, in vivo analysis of bacterial pathogenesis on Caenorhabditis elegans reveals a drastic increase in survival rates in FEE treated worms compared to untreated control. Conclusions. FEE showed promising QS inhibitory activity against P. aeruginosa . In the future, additional purification of crude FEE is required to remove carbohydrates, and pure isolated phytochemicals from FEE could be used as therapeutic agents to control QS-mediated infections in P. aeruginosa .

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Seagrass Halodule pinifolia active constituent 4-methoxybenzioic acid (4-MBA) inhibits quorum sensing mediated virulence production of Pseudomonas aeruginosa

Cited by 15 publications

References 44 publications

The Molecular Architecture of Pseudomonas aeruginosa Quorum-Sensing Inhibitors

The Molecular Architecture of Pseudomonas aeruginosa Quorum-Sensing Inhibitors

Exploring Oceans for Curative Compounds: Potential New Antimicrobial and Anti-Virulence Molecules against Pseudomonas aeruginosa

Exploration of the inhibitory effect of Cassia fistula on quorum sensing mediated virulence factor production and biofilm activity in Pseudomonas aeruginosa: an in vivo study in model organism Caenorhabditis elegans

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