Identifying the effect of thermal loads that are caused by temperature differences of inner and outer fluids on the structural sealing performance and strength is a long-standing quest in the design of subsea connectors. However, the structural stress and sealing parameters of the gasket under thermal loads are difficult to analyze and calculate using the current theoretical method because the boundary conditions of the gasket’s geometry surface are contact constraints interacted by the gasket and hubs. This paper presents an analytical calculation method (ACM) that evaluates the thermal-structural coupling strength and sealing performance considering such special boundary conditions. The thermal load is converted into an equivalent compression load, that is, a concentrated force, and applied to the contact region of the gasket. Additionally, a thermal-structural coupling finite element model is proposed to verify the ACM and the results show good agreement. Taken together, this work contributes to the design of subsea connectors that more likely take thermal loads into account.