Search for antimycobacterial constituents from a Tibetan medicinal plant, Gentianopsis paludosa

Abstract

From the whole plant of Gentianopsis paludosa seven compounds were isolated and identified to be 1,7-dihydroxy-3,8-dimethoxyxanthone (1), 1,7,8-trihydroxy-3-methoxyxanthone (2), 1-hydroxy-3,7,8-trimethoxyxanthone (3), 1,8-dihydroxy-2,6-dimethoxyxanthone (4), oleanolic acid (5), 4′ ′ ′ ′ ′,5,7-trihydroxyflavone (6) and luteolin-7-O-glucoside (7). Compounds 1, 2, 3 and 5 showed modest inhibitory effect against the growth of Mycobacterium smegmatis and M. tuberculosis.

Antibacterial activity of oleanolic and ursolic acids and their derivatives

“…Caliş and coworkers did not observe any antimicrobial activity of OA isolated from Cyclamen mirabile (Primulaceae) against Gram-positive (S. aureus and E. faecalis) and Gram-negative (P. aeruginosa and E. coli) species [50]. The antimicrobial constituents of Gentianopsis paludosa (Gentianaceae), the plant used in local Tibetan medicine to treat pneumonia, diarrhea and several other diseases were recently investigated and found to include OA, which when purified, was inactive against Mycobacterium smegmatis (MIC>500 mg mL -1 ) and M. tuberculosis (MIC>128 mg mL -1 ) [51].…”

Antimycobacterial activity of medicinal plants used by the Mayo people of Sonora, Mexico

“…MICs of >128 and >500 µg/mL against Mtb and Msm (Yeung et al, 2009). Daidzein showed a MIC >256 µg/mL against Msm (Lechner et al, 2008).…”

Profiling, Isolation, Chemical Characterisation and Distribution of Gentianaceae Constituents

“…Up to the present time, only one study has referred to the presence of a single 1,3,5-trioxygenated xanthone in Gentianopsis paludosa (Wang et al 2007). Otherwise, it is primarily 1,3,7,8-tetra-O-substituted xanthones that have been identified from Gentianopsis species, notably decussatin and gentiacaulein (Cheng et al 2010;Ding et al 2011;Ji et al 1992;Wang et al 2004;Yeung et al 2009). Based only on xanthone oxygenation patterns, there are clear analogies between Gentiana and Gentianopsis, such as the exclusive presence of 1,3,7 and 1,3,7,8 patterns, the absence of a higher degree of oxygenation and the frequent occurrence of primeverosyl moieties.…”

“…The C-glycoside mangiferin (1,3,6,7) was also reported, although this compound is present in various genera including Swertia and Gentianella. In contrast with other oxidation patterns, which exhibit quite a large diversity in substitutions, 1,3,6,7 O-substituted xanthones are represented almost exclusively by mangiferin, other 1,3,6,7 derivatives being seldom reported (Du et al 2012;Goetz and Jacot-Guillarmod 1977;Yeung et al 2009). Furthermore, it is interesting to note that mangiferin derivatives together with lancerin, a 4-β-D-glucopyranosyl-1,3,7-trihydroxyxanthone (Jankovic et al 2009;Lin et al 1982a), are the only C-glycoside xanthones present within the family Gentianaceae.…”